Lipopolysaccharide-induced Suppression of Graft-versus-Host Reactivity in Mice

Arie Molendijk; André C. Knulst; Ruud J.H.L.M. Van Gurp; Adri Van Oudenaren; Robbert Benner

doi:10.1016/S0171-2985(88)80005-6

Lipopolysaccharide-induced Suppression of Graft-versus-Host Reactivity in Mice

Arie Molendijk^*, André C. Knulst, Ruud J.H.L.M. Van Gurp, Adri Van Oudenaren, Robbert Benner

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Reconstitution of lethally irradiated mice with spleen cells from donors that had been treated with lipopolysaccharide (LPS) intravenously and allogeneic spleen cells subcutaneously leads to a suppressed anti-host delayed-type hypersensitivity (DTH). Either donor injection alone proved to be ineffective. The state of suppression appeared to be antigen-specific, but, depending on the experimental conditions, also anti-host DTH to third-party alloantigens could be suppressed. The suppression was mediated by a population of Thy-1^- suppressor cells that could also be induced in athymic nude mice. The suppressor cells specifically adhered to anti-kappa-coated plastic plates, but were not adsorbed by passage through a Sephadex G-10 column. Thus, it appears that the combined donor treatment with LPS and allogeneic spleen cells induces a population of B cells that can suppress anti-host immune reactivity.

Original language	English
Pages (from-to)	371-381
Number of pages	11
Journal	Immunobiology
Volume	177
Issue number	4-5
DOIs	https://doi.org/10.1016/S0171-2985(88)80005-6
Publication status	Published - 1988
Externally published	Yes

Keywords

balanced salt solution
BSS
delayed-type hypersensitivity
DTH
graft-versus-host
GvH
H
histocompatibility
host-
HvG

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10.1016/S0171-2985(88)80005-6

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title = "Lipopolysaccharide-induced Suppression of Graft-versus-Host Reactivity in Mice",

abstract = "Reconstitution of lethally irradiated mice with spleen cells from donors that had been treated with lipopolysaccharide (LPS) intravenously and allogeneic spleen cells subcutaneously leads to a suppressed anti-host delayed-type hypersensitivity (DTH). Either donor injection alone proved to be ineffective. The state of suppression appeared to be antigen-specific, but, depending on the experimental conditions, also anti-host DTH to third-party alloantigens could be suppressed. The suppression was mediated by a population of Thy-1- suppressor cells that could also be induced in athymic nude mice. The suppressor cells specifically adhered to anti-kappa-coated plastic plates, but were not adsorbed by passage through a Sephadex G-10 column. Thus, it appears that the combined donor treatment with LPS and allogeneic spleen cells induces a population of B cells that can suppress anti-host immune reactivity.",

keywords = "balanced salt solution, BSS, delayed-type hypersensitivity, DTH, graft-versus-host, GvH, H, histocompatibility, host-, HvG",

author = "Arie Molendijk and Knulst, \{Andr{\'e} C.\} and \{Van Gurp\}, \{Ruud J.H.L.M.\} and \{Van Oudenaren\}, Adri and Robbert Benner",

year = "1988",

doi = "10.1016/S0171-2985(88)80005-6",

language = "English",

volume = "177",

pages = "371--381",

journal = "Immunobiology",

issn = "0171-2985",

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T1 - Lipopolysaccharide-induced Suppression of Graft-versus-Host Reactivity in Mice

AU - Molendijk, Arie

AU - Knulst, André C.

AU - Van Gurp, Ruud J.H.L.M.

AU - Van Oudenaren, Adri

AU - Benner, Robbert

PY - 1988

Y1 - 1988

N2 - Reconstitution of lethally irradiated mice with spleen cells from donors that had been treated with lipopolysaccharide (LPS) intravenously and allogeneic spleen cells subcutaneously leads to a suppressed anti-host delayed-type hypersensitivity (DTH). Either donor injection alone proved to be ineffective. The state of suppression appeared to be antigen-specific, but, depending on the experimental conditions, also anti-host DTH to third-party alloantigens could be suppressed. The suppression was mediated by a population of Thy-1- suppressor cells that could also be induced in athymic nude mice. The suppressor cells specifically adhered to anti-kappa-coated plastic plates, but were not adsorbed by passage through a Sephadex G-10 column. Thus, it appears that the combined donor treatment with LPS and allogeneic spleen cells induces a population of B cells that can suppress anti-host immune reactivity.

AB - Reconstitution of lethally irradiated mice with spleen cells from donors that had been treated with lipopolysaccharide (LPS) intravenously and allogeneic spleen cells subcutaneously leads to a suppressed anti-host delayed-type hypersensitivity (DTH). Either donor injection alone proved to be ineffective. The state of suppression appeared to be antigen-specific, but, depending on the experimental conditions, also anti-host DTH to third-party alloantigens could be suppressed. The suppression was mediated by a population of Thy-1- suppressor cells that could also be induced in athymic nude mice. The suppressor cells specifically adhered to anti-kappa-coated plastic plates, but were not adsorbed by passage through a Sephadex G-10 column. Thus, it appears that the combined donor treatment with LPS and allogeneic spleen cells induces a population of B cells that can suppress anti-host immune reactivity.

KW - balanced salt solution

KW - BSS

KW - delayed-type hypersensitivity

KW - DTH

KW - graft-versus-host

KW - GvH

KW - H

KW - histocompatibility

KW - host-

KW - HvG

UR - https://www.scopus.com/pages/publications/0023790560

U2 - 10.1016/S0171-2985(88)80005-6

DO - 10.1016/S0171-2985(88)80005-6

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AN - SCOPUS:0023790560

SN - 0171-2985

VL - 177

SP - 371

EP - 381

JO - Immunobiology

JF - Immunobiology

IS - 4-5

ER -

Lipopolysaccharide-induced Suppression of Graft-versus-Host Reactivity in Mice

Abstract

Keywords

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