@inbook{8e89c11089f64ddca9f471322a98b6ae,
title = "Laser microdissection for spatial proteomics of postmortem brain tissue",
abstract = "Quantitative data of the proteome is highly valuable for providing unbiased information on protein expression changes related to disease or identifying related biomarkers. In brain diseases the affected area can be small and pathogenic events can be related to a specific cell type in an otherwise heterogeneous tissue type. An emerging approach dedicated to analysing this type of samples is laser microdissection (LMD) combined with LC-MS/MS into a single workflow. In this chapter, we describe two LMD methods for isolating tissue at the level of a small area and individual cells suitable for subsequent LC-MS/MS analysis.",
keywords = "Human brain, Immunohistochemistry, Laser capture, Laser microdissection, Mass spectrometry, Postmortem tissue, Proteomics, Single cell",
author = "Hondius, {David C.} and Hoozemans, {Jeroen J.M.} and Rozemuller, {Annemieke J.M.} and Smit, {August B.}",
note = "Funding Information: Xandy Peterson Pompa, Arizona Department of Health Services; Robert Deener, Berkley County Health Department; Brooke Bregman, Sebastian Chavez, Ellora Karmarkar, John J Openshaw, Hilary E Rosen, Rob Schechter, California Department of Public Health; Joann Gruber, CDC; Lea Kendrick, Children's Healthcare of Atlanta; Nisha Alden, Isaac Armistead, Alexis Burakoff, Rachel Herlihy, Breanna Kawasaki, Allison Mahon, Jackie West-Denning, Colorado Department of Public Health and Environment; Christopher L. Carroll, Connecticut Children's Medical Center; Lynn Sosa, Connecticut Department of Public Health; Paula Eggers, Division of Public Health, Delaware Health and Social Services; Douglas County Health Department and Two Rivers Public Health Department; Andi L. Shane MD, Preeti Jaggi, Christina A. Rostad, Matt Linam, Emory University School of Medicine and Children's Healthcare of Atlanta; Laurel Harduar Morano, Epidemic Intelligence Service, CDC; Melissa Tobin-D'Angelo, Siri Wilson, Kristina Lam, Georgia Department of Public Health; Hawaii Department of Health; Caitlin Pedati, Oluwakemi Oni, John Satre, Iowa Department of Public Health; Ngozi Ezike, Illinois Department of Public Health; Lindsay Weaver, Sally Hallyburton, Indiana State Department of Health; Justin Blanding, Kansas Department of Health and Environment; Kathy Terry, Janiper Kwak, Island Peer Review Organization, Inc; Stacy Davidson, Amanda Hunt, Kevin Spicer, Kentucky Department of Public Health; Moon Kim, Susan Hathaway, Candace Gragnani, Los Angeles County Department of Public Health; Julie Hand, Louisiana Department of Health; Catherine M Brown, Meagan Burns, Allison L Neeson, Catherine Reilly, Bureau of Infectious Disease and Laboratory Sciences, Massachusetts Department of Public Health; David Blythe, Monique Duwell, John Sweitzer, Brian Schuh, Anna C. Sick-Samuels, Maryland Department of Health; Justin Henderson, Sally Bidol, Cole Burkholder, Bureau of Epidemiology, Michigan Department of Health and Human Services; Kate Cleavinger, Bureau of Communicable Disease Control and Prevention, Missouri Department of Health and Senior Services; Cindy Allard, Mississippi State Department of Health; Jenna Lifshitz, Jihong Yao, Stella Tsai, New Jersey Department of Health; Janis Gonzales, Joseph T. Hicks, Annaliese Mayette, New Mexico Department of Health; Marcus Friedrich, New York State Department of Health; Mike Antwi, Robert Arciuolo, Dena Bushman, Vennus Ballen, Jennifer Baumgartner, Marie Dorsinville, Daniel Eiras, Ana Maria Fireteanu, Kelsey L. Kepler, Emily McGibbon, Natasha McIntosh, Christina Ng, Stephanie Ngai, Rachel Paneth-Pollak, Jyotsna Ramachandran, Emma Ruderman, Julia Schillinger, Aparna Shankar, Amita Toprani, Ann Winters, New York City Department of Health and Mental Hygiene; Erica Wilson, Lana Deyneka, North Carolina Department of Health and Human Services; Ohio Department of Health; Ozair Naqvi, Tamela Hamilton, Oklahoma State Department of Health; Melissa Sutton, Oregon Health Authority; Kimberly Kline, Pendleton County Health Department; Kumar Nalluswami, Allison Longenberger, Jonah M Long, Sharon M. Watkins, Bureau of Epidemiology, Pennsylvania Department of Health; Amanda Hartley, Cassandra Jones, Tennessee Department of Health; Jonathan M Kolsin, Texas Department of State Health Services; Elizabeth Groenweghe, Unified Government of Wyandotte County and Kansas City Public Health Department; Angela C. Dunn, Bree Barbeau, Rachelle Boulton, Utah Department of Health; Azizul Islam, Andrea Young, Heather Buysse, Nancy Bryant, Julia Dorsey, Stephanie Kellner, Sara Naramore, Nicole Sullivan, Virginia Department of Health; Kossia Dassie, Preetha Iyengar, Washington D.C. Department of Health; Marisa A D'Angeli, Lindsay M Horn, Washington State Department of Health; Lindsey Mason, West Virginia Department of Health and Human Resources; Thomas Haupt, Wisconsin Department of Health Services; Thomas Murray, Christina Murdzek, Kathy Krechevsky, Yale New Haven Children's Hospital. Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-9662-9_4",
language = "English",
series = "Neuromethods",
publisher = "Humana Press Inc.",
pages = "43--53",
booktitle = "Neuromethods",
}