Large‐scale production of extracellular vesicles: Report on the “massivEVs” ISEV workshop

Lucia Paolini; Marta Monguio-Tortajada; Marta Costa; Fabio Antenucci; Mario Barilani; Marta Clos-Sansalvador; Andre Cronemberger Andrade; Tom Driedonks; Sara Giancaterino; Stephanie Kronstadt; Rachel Mizenko; Muhammed Nawaz; Xabier Osteikoetxea; Carla Pereira; Surya Shrivastava; Anders Toftegaard Boysen; Simon van de Wakker; Martijn J C van Herwijnen; Xiaoqin Wang; Dionysios Watson; Mario Gimona; Maria Kaprakis-Liaskos; Konstantin Konstantinov; Sai Kiang Lim; Nicole C. Meisner-Kober; Michiel Stork; Peter Nejsum; Annalisa Radeghieri; Eva Rohde; Nicolas Touzet; Marca H M Wauben; Kenneth Witwer; Antonella Bongiovanni; Paolo Bergese

doi:10.1002/jex2.63

Large‐scale production of extracellular vesicles: Report on the “massivEVs” ISEV workshop

Lucia Paolini^*, Marta Monguio-Tortajada, Marta Costa, Fabio Antenucci, Mario Barilani, Marta Clos-Sansalvador, Andre Cronemberger Andrade, Tom Driedonks, Sara Giancaterino, Stephanie Kronstadt, Rachel Mizenko, Muhammed Nawaz, Xabier Osteikoetxea, Carla Pereira, Surya Shrivastava, Anders Toftegaard Boysen, Simon van de Wakker, Martijn J C van Herwijnen, Xiaoqin Wang, Dionysios WatsonMario Gimona, Maria Kaprakis-Liaskos, Konstantin Konstantinov, Sai Kiang Lim, Nicole C. Meisner-Kober, Michiel Stork, Peter Nejsum, Annalisa Radeghieri, Eva Rohde, Nicolas Touzet, Marca H M Wauben, Kenneth Witwer, Antonella Bongiovanni, Paolo Bergese

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Extracellular vesicles (EVs) large-scale production is a crucial point for the translation of EVs from discovery to application of EV-based products. In October
2021, the International Society for Extracellular Vesicles (ISEV), along with support by the FET-OPEN projects, “The Extracellular Vesicle Foundry” (evFOUNDRY)
and “Extracellular vesicles from a natural source for tailor-made nanomaterials”
(VES4US), organized a workshop entitled “massivEVs” to discuss the potential challenges for translation of EV-based products. This report gives an overview of the
topics discussed during “massivEVs”, the most important points raised, and the points
of consensus reached after discussion among academia and industry representatives.
Overall, the review of the existing EV manufacturing, upscaling challenges and directions for their resolution highlighted in the workshop painted an optimistic future for
the expanding EV field

Original language	English
Article number	e63
Number of pages	12
Journal	Journal of Extracellular Biology
Volume	1
Issue number	10
DOIs	https://doi.org/10.1002/jex2.63
Publication status	Published - Oct 2022

Keywords

EV-based products
ISEV workshop
extracellular vesicles
large-scale production
manufacturing
processing
regulatory issues

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Paolini, L., Monguio-Tortajada, M., Costa, M., Antenucci, F., Barilani, M., Clos-Sansalvador, M., Cronemberger Andrade, A., Driedonks, T., Giancaterino, S., Kronstadt, S., Mizenko, R., Nawaz, M., Osteikoetxea, X., Pereira, C., Shrivastava, S., Toftegaard Boysen, A., van de Wakker, S., van Herwijnen, M. J. C., Wang, X., ... Bergese, P. (2022). Large‐scale production of extracellular vesicles: Report on the “massivEVs” ISEV workshop. Journal of Extracellular Biology, 1(10), Article e63. https://doi.org/10.1002/jex2.63

@article{b173b37942314e8e9f8c6508d1b36269,

title = "Large‐scale production of extracellular vesicles: Report on the “massivEVs” ISEV workshop",

abstract = "Extracellular vesicles (EVs) large-scale production is a crucial point for the translation of EVs from discovery to application of EV-based products. In October2021, the International Society for Extracellular Vesicles (ISEV), along with support by the FET-OPEN projects, “The Extracellular Vesicle Foundry” (evFOUNDRY)and “Extracellular vesicles from a natural source for tailor-made nanomaterials”(VES4US), organized a workshop entitled “massivEVs” to discuss the potential challenges for translation of EV-based products. This report gives an overview of thetopics discussed during “massivEVs”, the most important points raised, and the pointsof consensus reached after discussion among academia and industry representatives.Overall, the review of the existing EV manufacturing, upscaling challenges and directions for their resolution highlighted in the workshop painted an optimistic future forthe expanding EV field",

keywords = "EV-based products, ISEV workshop, extracellular vesicles, large-scale production, manufacturing, processing, regulatory issues",

author = "Lucia Paolini and Marta Monguio-Tortajada and Marta Costa and Fabio Antenucci and Mario Barilani and Marta Clos-Sansalvador and {Cronemberger Andrade}, Andre and Tom Driedonks and Sara Giancaterino and Stephanie Kronstadt and Rachel Mizenko and Muhammed Nawaz and Xabier Osteikoetxea and Carla Pereira and Surya Shrivastava and {Toftegaard Boysen}, Anders and {van de Wakker}, Simon and {van Herwijnen}, {Martijn J C} and Xiaoqin Wang and Dionysios Watson and Mario Gimona and Maria Kaprakis-Liaskos and Konstantin Konstantinov and Lim, {Sai Kiang} and Meisner-Kober, {Nicole C.} and Michiel Stork and Peter Nejsum and Annalisa Radeghieri and Eva Rohde and Nicolas Touzet and Wauben, {Marca H M} and Kenneth Witwer and Antonella Bongiovanni and Paolo Bergese",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors. Journal of Extracellular Biology published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.",

year = "2022",

month = oct,

doi = "10.1002/jex2.63",

language = "English",

volume = "1",

number = "10",

}

Paolini, L, Monguio-Tortajada, M, Costa, M, Antenucci, F, Barilani, M, Clos-Sansalvador, M, Cronemberger Andrade, A, Driedonks, T, Giancaterino, S, Kronstadt, S, Mizenko, R, Nawaz, M, Osteikoetxea, X, Pereira, C, Shrivastava, S, Toftegaard Boysen, A, van de Wakker, S, van Herwijnen, MJC, Wang, X, Watson, D, Gimona, M, Kaprakis-Liaskos, M, Konstantinov, K, Lim, SK, Meisner-Kober, NC, Stork, M, Nejsum, P, Radeghieri, A, Rohde, E, Touzet, N, Wauben, MHM, Witwer, K, Bongiovanni, A & Bergese, P 2022, 'Large‐scale production of extracellular vesicles: Report on the “massivEVs” ISEV workshop', Journal of Extracellular Biology, vol. 1, no. 10, e63. https://doi.org/10.1002/jex2.63

TY - JOUR

T1 - Large‐scale production of extracellular vesicles

T2 - Report on the “massivEVs” ISEV workshop

AU - Paolini, Lucia

AU - Monguio-Tortajada, Marta

AU - Costa, Marta

AU - Antenucci, Fabio

AU - Barilani, Mario

AU - Clos-Sansalvador, Marta

AU - Cronemberger Andrade, Andre

AU - Driedonks, Tom

AU - Giancaterino, Sara

AU - Kronstadt, Stephanie

AU - Mizenko, Rachel

AU - Nawaz, Muhammed

AU - Osteikoetxea, Xabier

AU - Pereira, Carla

AU - Shrivastava, Surya

AU - Toftegaard Boysen, Anders

AU - van de Wakker, Simon

AU - van Herwijnen, Martijn J C

AU - Wang, Xiaoqin

AU - Watson, Dionysios

AU - Gimona, Mario

AU - Kaprakis-Liaskos, Maria

AU - Konstantinov, Konstantin

AU - Lim, Sai Kiang

AU - Meisner-Kober, Nicole C.

AU - Stork, Michiel

AU - Nejsum, Peter

AU - Radeghieri, Annalisa

AU - Rohde, Eva

AU - Touzet, Nicolas

AU - Wauben, Marca H M

AU - Witwer, Kenneth

AU - Bongiovanni, Antonella

AU - Bergese, Paolo

PY - 2022/10

Y1 - 2022/10

N2 - Extracellular vesicles (EVs) large-scale production is a crucial point for the translation of EVs from discovery to application of EV-based products. In October2021, the International Society for Extracellular Vesicles (ISEV), along with support by the FET-OPEN projects, “The Extracellular Vesicle Foundry” (evFOUNDRY)and “Extracellular vesicles from a natural source for tailor-made nanomaterials”(VES4US), organized a workshop entitled “massivEVs” to discuss the potential challenges for translation of EV-based products. This report gives an overview of thetopics discussed during “massivEVs”, the most important points raised, and the pointsof consensus reached after discussion among academia and industry representatives.Overall, the review of the existing EV manufacturing, upscaling challenges and directions for their resolution highlighted in the workshop painted an optimistic future forthe expanding EV field

AB - Extracellular vesicles (EVs) large-scale production is a crucial point for the translation of EVs from discovery to application of EV-based products. In October2021, the International Society for Extracellular Vesicles (ISEV), along with support by the FET-OPEN projects, “The Extracellular Vesicle Foundry” (evFOUNDRY)and “Extracellular vesicles from a natural source for tailor-made nanomaterials”(VES4US), organized a workshop entitled “massivEVs” to discuss the potential challenges for translation of EV-based products. This report gives an overview of thetopics discussed during “massivEVs”, the most important points raised, and the pointsof consensus reached after discussion among academia and industry representatives.Overall, the review of the existing EV manufacturing, upscaling challenges and directions for their resolution highlighted in the workshop painted an optimistic future forthe expanding EV field

KW - EV-based products

KW - ISEV workshop

KW - extracellular vesicles

KW - large-scale production

KW - manufacturing

KW - processing

KW - regulatory issues

U2 - 10.1002/jex2.63

DO - 10.1002/jex2.63

M3 - Article

C2 - 38939213

SN - 2768-2811

VL - 1

JO - Journal of Extracellular Biology

JF - Journal of Extracellular Biology

IS - 10

M1 - e63

ER -

Large‐scale production of extracellular vesicles: Report on the “massivEVs” ISEV workshop

Abstract

Keywords

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