Lack of Lymphatic Vessel Phenotype in LYVE-1/CD44 Double Knockout Mice

Mai X. Luong, Joshua Tam, Qingcong Lin, Jeroen Hagendoorn, Kathryn J. Moore, Timothy P. Padera, Brian Seed, Dai Fukumura, Raju Kucherlapati*, Rakesh K. Jain

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Lymphatic vessels play a key role in maintaining tissue-fluid homeostasis, immune surveillance and metastasis. The hyaluronan receptor, LYVE-1, is widely used as a molecular marker for adult and embryonic lymphatic endothelium, but its physiological functions have not yet been established in vivo. In agreement with a recent report, LYVE-1(-/-) mice, which are healthy and fertile, do not display any defects related to congenital abnormalities of the lymphatic system. One hypothesis for the absence of a phenotype in LYVE-1 null mice is that other hyaluronan receptors, such as CD44, may compensate for LYVE-1. To test this hypothesis, we created LYVE-1/CD44 double knockout mice with appropriate littermate controls. Lymphatic vessel structure and function, as determined by histological methods and intravital microscopy, show that LYVE-1(-/-), CD44(-/-) and LYVE-1(-/-)/CD44(-/-) mice are indistinguishable from wild-type mice under normal conditions. Furthermore, resolution of carrageenan-induced paw edema is comparable in all genotypes. However, LYVE-1(-/-)/CD44-/- mice exhibit increased edema formation in a carrageenan-induced paw inflammation model compared to wild-type mice, but not to LYVE(-/-) or CD44(-/-) mice. These data suggest that LYVE-1 and CD44 are not required for the formation or function of lymphatics, but do not rule out a role for LYVE-1 in inflammation.

Original languageEnglish
Pages (from-to)430-437
Number of pages8
JournalJournal of Cellular Physiology
Volume219
Issue number2
DOIs
Publication statusPublished - May 2009

Keywords

  • Animals
  • Antigens, CD44
  • Embryonic Stem Cells
  • Female
  • Genotype
  • Glycoproteins
  • Lymphatic Vessels
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Phenotype
  • Journal Article
  • Research Support, N.I.H., Extramural

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