Lack of involvement of CD63 and CD9 tetraspanins in the extracellular vesicle content delivery process

Maria Laura Tognoli; Julia Dancourt; Emeline Bonsergent; Roberta Palmulli; Olivier G. de Jong; Guillaume Van Niel; Eric Rubinstein; Pieter Vader; Gregory Lavieu

doi:10.1038/s42003-023-04911-1

Lack of involvement of CD63 and CD9 tetraspanins in the extracellular vesicle content delivery process

Maria Laura Tognoli, Julia Dancourt, Emeline Bonsergent, Roberta Palmulli, Olivier G. de Jong, Guillaume Van Niel, Eric Rubinstein, Pieter Vader^*, Gregory Lavieu^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Extracellular vesicles (EVs) are thought to mediate intercellular communication by transferring cargoes from donor to acceptor cells. The EV content-delivery process within acceptor cells is still poorly characterized and debated. CD63 and CD9, members of the tetraspanin family, are highly enriched within EV membranes and are respectively enriched within multivesicular bodies/endosomes and at the plasma membrane of the cells. CD63 and CD9 have been suspected to regulate the EV uptake and delivery process. Here we used two independent assays and different cell models (HeLa, MDA-MB-231 and HEK293T cells) to assess the putative role of CD63 and CD9 in the EV delivery process that includes uptake and cargo delivery. Our results suggest that neither CD63, nor CD9 are required for this function.

Original language	English
Article number	532
Journal	Communications biology
Volume	6
Issue number	1
DOIs	https://doi.org/10.1038/s42003-023-04911-1
Publication status	Published - Dec 2023

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title = "Lack of involvement of CD63 and CD9 tetraspanins in the extracellular vesicle content delivery process",

abstract = "Extracellular vesicles (EVs) are thought to mediate intercellular communication by transferring cargoes from donor to acceptor cells. The EV content-delivery process within acceptor cells is still poorly characterized and debated. CD63 and CD9, members of the tetraspanin family, are highly enriched within EV membranes and are respectively enriched within multivesicular bodies/endosomes and at the plasma membrane of the cells. CD63 and CD9 have been suspected to regulate the EV uptake and delivery process. Here we used two independent assays and different cell models (HeLa, MDA-MB-231 and HEK293T cells) to assess the putative role of CD63 and CD9 in the EV delivery process that includes uptake and cargo delivery. Our results suggest that neither CD63, nor CD9 are required for this function.",

author = "Tognoli, {Maria Laura} and Julia Dancourt and Emeline Bonsergent and Roberta Palmulli and {de Jong}, {Olivier G.} and {Van Niel}, Guillaume and Eric Rubinstein and Pieter Vader and Gregory Lavieu",

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year = "2023",

month = dec,

doi = "10.1038/s42003-023-04911-1",

language = "English",

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T1 - Lack of involvement of CD63 and CD9 tetraspanins in the extracellular vesicle content delivery process

AU - Tognoli, Maria Laura

AU - Dancourt, Julia

AU - Bonsergent, Emeline

AU - Palmulli, Roberta

AU - de Jong, Olivier G.

AU - Van Niel, Guillaume

AU - Rubinstein, Eric

AU - Vader, Pieter

AU - Lavieu, Gregory

PY - 2023/12

Y1 - 2023/12

N2 - Extracellular vesicles (EVs) are thought to mediate intercellular communication by transferring cargoes from donor to acceptor cells. The EV content-delivery process within acceptor cells is still poorly characterized and debated. CD63 and CD9, members of the tetraspanin family, are highly enriched within EV membranes and are respectively enriched within multivesicular bodies/endosomes and at the plasma membrane of the cells. CD63 and CD9 have been suspected to regulate the EV uptake and delivery process. Here we used two independent assays and different cell models (HeLa, MDA-MB-231 and HEK293T cells) to assess the putative role of CD63 and CD9 in the EV delivery process that includes uptake and cargo delivery. Our results suggest that neither CD63, nor CD9 are required for this function.

AB - Extracellular vesicles (EVs) are thought to mediate intercellular communication by transferring cargoes from donor to acceptor cells. The EV content-delivery process within acceptor cells is still poorly characterized and debated. CD63 and CD9, members of the tetraspanin family, are highly enriched within EV membranes and are respectively enriched within multivesicular bodies/endosomes and at the plasma membrane of the cells. CD63 and CD9 have been suspected to regulate the EV uptake and delivery process. Here we used two independent assays and different cell models (HeLa, MDA-MB-231 and HEK293T cells) to assess the putative role of CD63 and CD9 in the EV delivery process that includes uptake and cargo delivery. Our results suggest that neither CD63, nor CD9 are required for this function.

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DO - 10.1038/s42003-023-04911-1

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JO - Communications biology

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Lack of involvement of CD63 and CD9 tetraspanins in the extracellular vesicle content delivery process

Abstract

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