Kin recognition between medial golgi enzymes in HeLa cells

Tommy Nilsson; Mee H. Hoe; Paul Slusarewicz; Catherine Rabouille; Rose Watson; Felicia Hunte; Gabriele Watzele; Eric G. Berger; Graham Warren

Kin recognition between medial golgi enzymes in HeLa cells

Tommy Nilsson, Mee H. Hoe, Paul Slusarewicz, Catherine Rabouille, Rose Watson, Felicia Hunte, Gabriele Watzele, Eric G. Berger, Graham Warren^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

213 Citations (Scopus)

Abstract

The medial Golgi enzymes, N-acetylglucosaminyltransferase I (NAGT I) and mannosidase II (Mann II), and the trans Golgi enzyme, β-1,4-galactosyltransferase (GalT) were each retained in the endoplasmic reticulum (ER) by grafting on the cytoplasmic tail of the p33 invariant chain. Transient and stable expression of p33/NAGT I in HeLa cells caused relocation of endogenous Mann II to the ER and transient expression of p33/Mann II had a similar effect on endogenous NAGT I. Neither of these endogenous medial enzymes were affected by transient expression of p33/GalT. These data provide strong evidence for kin recognition between medial Golgi enzymes and suggest a role for them in the organization of the Golgi stack.

Original language	English
Pages (from-to)	562-574
Number of pages	13
Journal	EMBO Journal
Volume	13
Issue number	3
Publication status	Published - 1 Feb 1994

Keywords

β-1,4-galactosyltransferase
Endoplasmic reticulum
Golgi
Mannosidase II
N-acetylglucosaminyltransferase I

Cite this

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title = "Kin recognition between medial golgi enzymes in HeLa cells",

abstract = "The medial Golgi enzymes, N-acetylglucosaminyltransferase I (NAGT I) and mannosidase II (Mann II), and the trans Golgi enzyme, β-1,4-galactosyltransferase (GalT) were each retained in the endoplasmic reticulum (ER) by grafting on the cytoplasmic tail of the p33 invariant chain. Transient and stable expression of p33/NAGT I in HeLa cells caused relocation of endogenous Mann II to the ER and transient expression of p33/Mann II had a similar effect on endogenous NAGT I. Neither of these endogenous medial enzymes were affected by transient expression of p33/GalT. These data provide strong evidence for kin recognition between medial Golgi enzymes and suggest a role for them in the organization of the Golgi stack.",

keywords = "β-1,4-galactosyltransferase, Endoplasmic reticulum, Golgi, Mannosidase II, N-acetylglucosaminyltransferase I",

author = "Tommy Nilsson and Hoe, {Mee H.} and Paul Slusarewicz and Catherine Rabouille and Rose Watson and Felicia Hunte and Gabriele Watzele and Berger, {Eric G.} and Graham Warren",

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TY - JOUR

T1 - Kin recognition between medial golgi enzymes in HeLa cells

AU - Nilsson, Tommy

AU - Hoe, Mee H.

AU - Slusarewicz, Paul

AU - Rabouille, Catherine

AU - Watson, Rose

AU - Hunte, Felicia

AU - Watzele, Gabriele

AU - Berger, Eric G.

AU - Warren, Graham

PY - 1994/2/1

Y1 - 1994/2/1

N2 - The medial Golgi enzymes, N-acetylglucosaminyltransferase I (NAGT I) and mannosidase II (Mann II), and the trans Golgi enzyme, β-1,4-galactosyltransferase (GalT) were each retained in the endoplasmic reticulum (ER) by grafting on the cytoplasmic tail of the p33 invariant chain. Transient and stable expression of p33/NAGT I in HeLa cells caused relocation of endogenous Mann II to the ER and transient expression of p33/Mann II had a similar effect on endogenous NAGT I. Neither of these endogenous medial enzymes were affected by transient expression of p33/GalT. These data provide strong evidence for kin recognition between medial Golgi enzymes and suggest a role for them in the organization of the Golgi stack.

AB - The medial Golgi enzymes, N-acetylglucosaminyltransferase I (NAGT I) and mannosidase II (Mann II), and the trans Golgi enzyme, β-1,4-galactosyltransferase (GalT) were each retained in the endoplasmic reticulum (ER) by grafting on the cytoplasmic tail of the p33 invariant chain. Transient and stable expression of p33/NAGT I in HeLa cells caused relocation of endogenous Mann II to the ER and transient expression of p33/Mann II had a similar effect on endogenous NAGT I. Neither of these endogenous medial enzymes were affected by transient expression of p33/GalT. These data provide strong evidence for kin recognition between medial Golgi enzymes and suggest a role for them in the organization of the Golgi stack.

KW - β-1,4-galactosyltransferase

KW - Endoplasmic reticulum

KW - Golgi

KW - Mannosidase II

KW - N-acetylglucosaminyltransferase I

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M3 - Article

C2 - 8313901

AN - SCOPUS:0028047115

SN - 0261-4189

VL - 13

SP - 562

EP - 574

JO - EMBO Journal

JF - EMBO Journal

IS - 3

ER -

Kin recognition between medial golgi enzymes in HeLa cells

Abstract

Keywords

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