Isolation and regional localization of 25 anonymous dna probes on a chromosome 13 hybrid panel

R. H.J. Houwen; S. E. Pautler; J. A. Barwell; K. Arden; J. A. Buchanan; C. D. James; W. K. Cavenee; C. H.C.M. Buys; J. K. Cowell; D. W. Cox

doi:10.1159/000133120

Isolation and regional localization of 25 anonymous dna probes on a chromosome 13 hybrid panel

R. H.J. Houwen, S. E. Pautler, J. A. Barwell, K. Arden, J. A. Buchanan, C. D. James, W. K. Cavenee, C. H.C.M. Buys, J. K. Cowell, D. W. Cox^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

14 Citations (Scopus)

Abstract

Clones were isolated from two flow-sorted chromosome 13 libraries. Twenty-five clones were localized to various regions of chromosome 13, using a well-characterized panel of rodent x human hybrid cell lines. Eight DNA markers were localized to 13q14.2→q22, where the gene for Wilson disease, a recessive disorder of copper metabolism, was previously assigned. The new markers will be useful for the diagnosis of pre-symptomatic sibs of Wilson disease patients. We isolated six DNA clones proximal to the retinoblastoma gene, a region in which a translocation associated with rhabdomyosarcoma has been observed. Probes for both of these regions will be useful for the cloning of the genes involved in these diseases.

Original language	English
Pages (from-to)	87-90
Number of pages	4
Journal	Cytogenetic and Genome Research
Volume	57
Issue number	2-3
DOIs	https://doi.org/10.1159/000133120
Publication status	Published - 1 Jan 1991

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title = "Isolation and regional localization of 25 anonymous dna probes on a chromosome 13 hybrid panel",

abstract = "Clones were isolated from two flow-sorted chromosome 13 libraries. Twenty-five clones were localized to various regions of chromosome 13, using a well-characterized panel of rodent x human hybrid cell lines. Eight DNA markers were localized to 13q14.2→q22, where the gene for Wilson disease, a recessive disorder of copper metabolism, was previously assigned. The new markers will be useful for the diagnosis of pre-symptomatic sibs of Wilson disease patients. We isolated six DNA clones proximal to the retinoblastoma gene, a region in which a translocation associated with rhabdomyosarcoma has been observed. Probes for both of these regions will be useful for the cloning of the genes involved in these diseases.",

author = "Houwen, {R. H.J.} and Pautler, {S. E.} and Barwell, {J. A.} and K. Arden and Buchanan, {J. A.} and James, {C. D.} and Cavenee, {W. K.} and Buys, {C. H.C.M.} and Cowell, {J. K.} and Cox, {D. W.}",

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AU - Houwen, R. H.J.

AU - Pautler, S. E.

AU - Barwell, J. A.

AU - Arden, K.

AU - Buchanan, J. A.

AU - James, C. D.

AU - Cavenee, W. K.

AU - Buys, C. H.C.M.

AU - Cowell, J. K.

AU - Cox, D. W.

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AB - Clones were isolated from two flow-sorted chromosome 13 libraries. Twenty-five clones were localized to various regions of chromosome 13, using a well-characterized panel of rodent x human hybrid cell lines. Eight DNA markers were localized to 13q14.2→q22, where the gene for Wilson disease, a recessive disorder of copper metabolism, was previously assigned. The new markers will be useful for the diagnosis of pre-symptomatic sibs of Wilson disease patients. We isolated six DNA clones proximal to the retinoblastoma gene, a region in which a translocation associated with rhabdomyosarcoma has been observed. Probes for both of these regions will be useful for the cloning of the genes involved in these diseases.

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Isolation and regional localization of 25 anonymous dna probes on a chromosome 13 hybrid panel

Abstract

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