Islet amyloid polypeptide (IAPP) is synthesized in the islets of Langerhans. Detection of IAPP polypeptide and IAPP mRNA by combined in situ hybridization and immunohistochemistry in rat pancreas

M Denijn; R A De Weger; A D Van Mansfeld; J A van Unnik; C J Lips

Islet amyloid polypeptide (IAPP) is synthesized in the islets of Langerhans. Detection of IAPP polypeptide and IAPP mRNA by combined in situ hybridization and immunohistochemistry in rat pancreas

M Denijn, R A De Weger, A D Van Mansfeld, J A van Unnik, C J Lips

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

We investigated the localization of IAPP mRNA by means of in situ hybridization in tissue sections of rat pancreas. A 35S-labeled, IAPP-specific DNA probe--hybridized specifically in the islets of Langerhans. This localization was confirmed by immunohistochemical localization of insulin and IAPP polypeptides on adjacent tissue sections. Moreover, combined in situ hybridization of IAPP mRNA and immunohistochemistry of insulin and IAPP polypeptide on the same section, using insulin as specific marker shows the presence of IAPP mRNA in the islets of Langerhans.

Original language	English
Pages (from-to)	33-7
Number of pages	5
Journal	Histochemistry
Volume	97
Issue number	1
Publication status	Published - 1992

Keywords

Amyloid
Animals
Immunohistochemistry
Insulin
Islet Amyloid Polypeptide
Islets of Langerhans
Nucleic Acid Hybridization
Pancreas
RNA, Messenger
Rats
Rats, Inbred Strains

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@article{5bf8da6769af4bc688a83948dff63808,

title = "Islet amyloid polypeptide (IAPP) is synthesized in the islets of Langerhans. Detection of IAPP polypeptide and IAPP mRNA by combined in situ hybridization and immunohistochemistry in rat pancreas",

abstract = "We investigated the localization of IAPP mRNA by means of in situ hybridization in tissue sections of rat pancreas. A 35S-labeled, IAPP-specific DNA probe--hybridized specifically in the islets of Langerhans. This localization was confirmed by immunohistochemical localization of insulin and IAPP polypeptides on adjacent tissue sections. Moreover, combined in situ hybridization of IAPP mRNA and immunohistochemistry of insulin and IAPP polypeptide on the same section, using insulin as specific marker shows the presence of IAPP mRNA in the islets of Langerhans.",

keywords = "Amyloid, Animals, Immunohistochemistry, Insulin, Islet Amyloid Polypeptide, Islets of Langerhans, Nucleic Acid Hybridization, Pancreas, RNA, Messenger, Rats, Rats, Inbred Strains",

author = "M Denijn and {De Weger}, {R A} and {Van Mansfeld}, {A D} and {van Unnik}, {J A} and Lips, {C J}",

year = "1992",

language = "English",

volume = "97",

pages = "33--7",

number = "1",

}

Islet amyloid polypeptide (IAPP) is synthesized in the islets of Langerhans. Detection of IAPP polypeptide and IAPP mRNA by combined in situ hybridization and immunohistochemistry in rat pancreas. / Denijn, M; De Weger, R A; Van Mansfeld, A D et al.
In: Histochemistry, Vol. 97, No. 1, 1992, p. 33-7.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Islet amyloid polypeptide (IAPP) is synthesized in the islets of Langerhans. Detection of IAPP polypeptide and IAPP mRNA by combined in situ hybridization and immunohistochemistry in rat pancreas

AU - Denijn, M

AU - De Weger, R A

AU - Van Mansfeld, A D

AU - van Unnik, J A

AU - Lips, C J

PY - 1992

Y1 - 1992

N2 - We investigated the localization of IAPP mRNA by means of in situ hybridization in tissue sections of rat pancreas. A 35S-labeled, IAPP-specific DNA probe--hybridized specifically in the islets of Langerhans. This localization was confirmed by immunohistochemical localization of insulin and IAPP polypeptides on adjacent tissue sections. Moreover, combined in situ hybridization of IAPP mRNA and immunohistochemistry of insulin and IAPP polypeptide on the same section, using insulin as specific marker shows the presence of IAPP mRNA in the islets of Langerhans.

AB - We investigated the localization of IAPP mRNA by means of in situ hybridization in tissue sections of rat pancreas. A 35S-labeled, IAPP-specific DNA probe--hybridized specifically in the islets of Langerhans. This localization was confirmed by immunohistochemical localization of insulin and IAPP polypeptides on adjacent tissue sections. Moreover, combined in situ hybridization of IAPP mRNA and immunohistochemistry of insulin and IAPP polypeptide on the same section, using insulin as specific marker shows the presence of IAPP mRNA in the islets of Langerhans.

KW - Amyloid

KW - Animals

KW - Immunohistochemistry

KW - Insulin

KW - Islet Amyloid Polypeptide

KW - Islets of Langerhans

KW - Nucleic Acid Hybridization

KW - Pancreas

KW - RNA, Messenger

KW - Rats

KW - Rats, Inbred Strains

M3 - Article

C2 - 1618637

SN - 0301-5564

VL - 97

SP - 33

EP - 37

JO - Histochemistry

JF - Histochemistry

IS - 1

ER -

Islet amyloid polypeptide (IAPP) is synthesized in the islets of Langerhans. Detection of IAPP polypeptide and IAPP mRNA by combined in situ hybridization and immunohistochemistry in rat pancreas

Abstract

Keywords

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