Interaction of extracellular vesicles with endothelial cells under physiological flow conditions

Susan M. van Dommelen, Margaret Fish, Arjan D Barendrecht, Raymond M. Schiffelers, Omolola Eniola-Adefeso, Pieter Vader*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review


In the last few years it has become clear that, in addition to soluble molecules such as growth factors and cytokines, cells use extracellular vesicles (EVs) for intercellular communication. For example, EVs derived from cancer cells interact with endothelial cells, thereby affecting angiogenesis and metastasis, two essential processes in tumor progression. In most experiments, the interaction of EVs with target cells is investigated under static conditions. However the use of dynamic flow conditions is considered more relevant, especially when studying EV uptake by endothelial cells. Here, we describe the use of a perfusion system to investigate the interaction of (tumor) EVs with endothelial cells under dynamic flow conditions.

Original languageEnglish
Title of host publicationExosomes and Microvesicles
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages9
ISBN (Electronic)978-1-4939-6728-5
ISBN (Print)978-1-4939-6726-1
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Binding
  • Endothelial cells
  • Extracellular vesicles
  • Physiological flow
  • Targeting
  • Uptake


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