Interaction of extracellular vesicles with endothelial cells under physiological flow conditions

Susan M. van Dommelen; Margaret Fish; Arjan D Barendrecht; Raymond M. Schiffelers; Omolola Eniola-Adefeso; Pieter Vader

doi:10.1007/978-1-4939-6728-5_15

Interaction of extracellular vesicles with endothelial cells under physiological flow conditions

Susan M. van Dommelen, Margaret Fish, Arjan D Barendrecht, Raymond M. Schiffelers, Omolola Eniola-Adefeso, Pieter Vader^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

Abstract

In the last few years it has become clear that, in addition to soluble molecules such as growth factors and cytokines, cells use extracellular vesicles (EVs) for intercellular communication. For example, EVs derived from cancer cells interact with endothelial cells, thereby affecting angiogenesis and metastasis, two essential processes in tumor progression. In most experiments, the interaction of EVs with target cells is investigated under static conditions. However the use of dynamic flow conditions is considered more relevant, especially when studying EV uptake by endothelial cells. Here, we describe the use of a perfusion system to investigate the interaction of (tumor) EVs with endothelial cells under dynamic flow conditions.

Original language	English
Title of host publication	Exosomes and Microvesicles
Subtitle of host publication	Methods and Protocols
Publisher	Humana Press Inc.
Pages	205-213
Number of pages	9
ISBN (Electronic)	978-1-4939-6728-5
ISBN (Print)	978-1-4939-6726-1
DOIs	https://doi.org/10.1007/978-1-4939-6728-5_15
Publication status	Published - 2017

Publication series

Name	Methods in Molecular Biology
Volume	1545
ISSN (Print)	1064-3745

Keywords

Binding
Endothelial cells
Extracellular vesicles
Physiological flow
Targeting
Uptake

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10.1007/978-1-4939-6728-5_15

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van Dommelen, S. M., Fish, M., Barendrecht, A. D., Schiffelers, R. M., Eniola-Adefeso, O., & Vader, P. (2017). Interaction of extracellular vesicles with endothelial cells under physiological flow conditions. In Exosomes and Microvesicles: Methods and Protocols (pp. 205-213). (Methods in Molecular Biology; Vol. 1545). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6728-5_15

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title = "Interaction of extracellular vesicles with endothelial cells under physiological flow conditions",

abstract = "In the last few years it has become clear that, in addition to soluble molecules such as growth factors and cytokines, cells use extracellular vesicles (EVs) for intercellular communication. For example, EVs derived from cancer cells interact with endothelial cells, thereby affecting angiogenesis and metastasis, two essential processes in tumor progression. In most experiments, the interaction of EVs with target cells is investigated under static conditions. However the use of dynamic flow conditions is considered more relevant, especially when studying EV uptake by endothelial cells. Here, we describe the use of a perfusion system to investigate the interaction of (tumor) EVs with endothelial cells under dynamic flow conditions.",

keywords = "Binding, Endothelial cells, Extracellular vesicles, Physiological flow, Targeting, Uptake",

author = "{van Dommelen}, {Susan M.} and Margaret Fish and Barendrecht, {Arjan D} and Schiffelers, {Raymond M.} and Omolola Eniola-Adefeso and Pieter Vader",

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doi = "10.1007/978-1-4939-6728-5_15",

language = "English",

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Interaction of extracellular vesicles with endothelial cells under physiological flow conditions. / van Dommelen, Susan M.; Fish, Margaret; Barendrecht, Arjan D et al.
Exosomes and Microvesicles: Methods and Protocols. Humana Press Inc., 2017. p. 205-213 (Methods in Molecular Biology; Vol. 1545).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

TY - CHAP

T1 - Interaction of extracellular vesicles with endothelial cells under physiological flow conditions

AU - van Dommelen, Susan M.

AU - Fish, Margaret

AU - Barendrecht, Arjan D

AU - Schiffelers, Raymond M.

AU - Eniola-Adefeso, Omolola

AU - Vader, Pieter

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AB - In the last few years it has become clear that, in addition to soluble molecules such as growth factors and cytokines, cells use extracellular vesicles (EVs) for intercellular communication. For example, EVs derived from cancer cells interact with endothelial cells, thereby affecting angiogenesis and metastasis, two essential processes in tumor progression. In most experiments, the interaction of EVs with target cells is investigated under static conditions. However the use of dynamic flow conditions is considered more relevant, especially when studying EV uptake by endothelial cells. Here, we describe the use of a perfusion system to investigate the interaction of (tumor) EVs with endothelial cells under dynamic flow conditions.

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KW - Endothelial cells

KW - Extracellular vesicles

KW - Physiological flow

KW - Targeting

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T3 - Methods in Molecular Biology

SP - 205

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BT - Exosomes and Microvesicles

PB - Humana Press Inc.

ER -

Interaction of extracellular vesicles with endothelial cells under physiological flow conditions

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Keywords

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