Inter-domain Cooperation in INCENP Promotes Aurora B Relocation from Centromeres to Microtubules

Armando van der Horst, Martijn J M Vromans, Kim Bouwman, Maike S van der Waal, Michael A Hadders, Susanne M A Lens

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The chromosomal passenger complex is essential for error-free chromosome segregation and proper execution of cytokinesis. To coordinate nuclear division with cytoplasmic division, its enzymatic subunit, Aurora B, relocalizes from centromeres in metaphase to the spindle midzone in anaphase. In budding yeast, this requires dephosphorylation of the microtubule-binding (MTB) domain of the INCENP analog Sli15. The mechanistic basis for this relocalization in metazoans is incompletely understood. We demonstrate that the putative coiled-coil domain within INCENP drives midzone localization of Aurora B via a direct, electrostatic interaction with microtubules. Furthermore, we provide evidence that the CPC multimerizes via INCENP's centromere-targeting domain (CEN box), which increases the MTB affinity of INCENP. In (pro)metaphase, the MTB affinity of INCENP is outcompeted by the affinity of its CEN box for centromeres, while at anaphase onset—when the histone mark H2AT120 is dephosphorylated—INCENP and Aurora B switch from centromere to microtubule localization.

Original languageEnglish
Pages (from-to)380-387
Number of pages8
JournalCell Reports [E]
Volume12
Issue number3
DOIs
Publication statusPublished - 21 Jul 2015

Fingerprint

Dive into the research topics of 'Inter-domain Cooperation in INCENP Promotes Aurora B Relocation from Centromeres to Microtubules'. Together they form a unique fingerprint.

Cite this