Integrin modulating factor-1: A lipid that alters the function of leukocyte integrins

Anne Hermanowski-Vosatka; Jos A.G. Van Strijp; William J. Swiggard; Samuel D. Wright

doi:10.1016/0092-8674(92)90475-R

Integrin modulating factor-1: A lipid that alters the function of leukocyte integrins

Anne Hermanowski-Vosatka^*
, Jos A.G. Van Strijp
, William J. Swiggard
, Samuel D. Wright

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

126 Citations (Scopus)

Abstract

The avidity of integrin CR3 (also known as α_Mβ₂, Mac-1, Mo-1, and CD11b CD18) may be reversibly altered without changes in the number of cell surface receptors. Here we describe a molecule termed integrin modulating factor (IMF-1), which controls CR3 avidity. Addition of IMF-1 to polymorphonuclear leukocytes (PMNs) or to purified CR3 causes enhanced binding of ligand. IMF-1 is not present in resting PMNs, but stimulation of cells results in a transient rise in IMF-1 content that parallels a transient rise in CR3 activity. We suggest that PMNs control adhesivity by controlling synthesis of IMF-1, which then acts as an allosteric activator of leukocyte integrins. IMF-1 is an acidic, amphiphilic molecule of M_r340 ± 16 that does not contain ester, phosphate, amide, sialic acid, or glycosidic or vicinal hydroxyl functionalities, but does contain a carbon-carbon double bond. These results suggest that IMF-1 is an unsaturated fatty acid or an isoprenoid acid.

Original language	English
Pages (from-to)	341-352
Number of pages	12
Journal	Cell
Volume	68
Issue number	2
DOIs	https://doi.org/10.1016/0092-8674(92)90475-R
Publication status	Published - 24 Jan 1992

Access to Document

10.1016/0092-8674(92)90475-R

Cite this

@article{a4df71df5e3e4d1e99504fd45a90f8f4,

title = "Integrin modulating factor-1: A lipid that alters the function of leukocyte integrins",

abstract = "The avidity of integrin CR3 (also known as αMβ2, Mac-1, Mo-1, and CD11b CD18) may be reversibly altered without changes in the number of cell surface receptors. Here we describe a molecule termed integrin modulating factor (IMF-1), which controls CR3 avidity. Addition of IMF-1 to polymorphonuclear leukocytes (PMNs) or to purified CR3 causes enhanced binding of ligand. IMF-1 is not present in resting PMNs, but stimulation of cells results in a transient rise in IMF-1 content that parallels a transient rise in CR3 activity. We suggest that PMNs control adhesivity by controlling synthesis of IMF-1, which then acts as an allosteric activator of leukocyte integrins. IMF-1 is an acidic, amphiphilic molecule of Mr340 ± 16 that does not contain ester, phosphate, amide, sialic acid, or glycosidic or vicinal hydroxyl functionalities, but does contain a carbon-carbon double bond. These results suggest that IMF-1 is an unsaturated fatty acid or an isoprenoid acid.",

author = "Anne Hermanowski-Vosatka and \{Van Strijp\}, \{Jos A.G.\} and Swiggard, \{William J.\} and Wright, \{Samuel D.\}",

year = "1992",

month = jan,

day = "24",

doi = "10.1016/0092-8674(92)90475-R",

language = "English",

volume = "68",

pages = "341--352",

journal = "Cell",

issn = "0092-8674",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Integrin modulating factor-1

T2 - A lipid that alters the function of leukocyte integrins

AU - Hermanowski-Vosatka, Anne

AU - Van Strijp, Jos A.G.

AU - Swiggard, William J.

AU - Wright, Samuel D.

PY - 1992/1/24

Y1 - 1992/1/24

N2 - The avidity of integrin CR3 (also known as αMβ2, Mac-1, Mo-1, and CD11b CD18) may be reversibly altered without changes in the number of cell surface receptors. Here we describe a molecule termed integrin modulating factor (IMF-1), which controls CR3 avidity. Addition of IMF-1 to polymorphonuclear leukocytes (PMNs) or to purified CR3 causes enhanced binding of ligand. IMF-1 is not present in resting PMNs, but stimulation of cells results in a transient rise in IMF-1 content that parallels a transient rise in CR3 activity. We suggest that PMNs control adhesivity by controlling synthesis of IMF-1, which then acts as an allosteric activator of leukocyte integrins. IMF-1 is an acidic, amphiphilic molecule of Mr340 ± 16 that does not contain ester, phosphate, amide, sialic acid, or glycosidic or vicinal hydroxyl functionalities, but does contain a carbon-carbon double bond. These results suggest that IMF-1 is an unsaturated fatty acid or an isoprenoid acid.

AB - The avidity of integrin CR3 (also known as αMβ2, Mac-1, Mo-1, and CD11b CD18) may be reversibly altered without changes in the number of cell surface receptors. Here we describe a molecule termed integrin modulating factor (IMF-1), which controls CR3 avidity. Addition of IMF-1 to polymorphonuclear leukocytes (PMNs) or to purified CR3 causes enhanced binding of ligand. IMF-1 is not present in resting PMNs, but stimulation of cells results in a transient rise in IMF-1 content that parallels a transient rise in CR3 activity. We suggest that PMNs control adhesivity by controlling synthesis of IMF-1, which then acts as an allosteric activator of leukocyte integrins. IMF-1 is an acidic, amphiphilic molecule of Mr340 ± 16 that does not contain ester, phosphate, amide, sialic acid, or glycosidic or vicinal hydroxyl functionalities, but does contain a carbon-carbon double bond. These results suggest that IMF-1 is an unsaturated fatty acid or an isoprenoid acid.

UR - https://www.scopus.com/pages/publications/0026527577

U2 - 10.1016/0092-8674(92)90475-R

DO - 10.1016/0092-8674(92)90475-R

M3 - Article

C2 - 1733503

AN - SCOPUS:0026527577

SN - 0092-8674

VL - 68

SP - 341

EP - 352

JO - Cell

JF - Cell

IS - 2

ER -

Integrin modulating factor-1: A lipid that alters the function of leukocyte integrins

Abstract

Access to Document

Other files and links

Fingerprint

Cite this