TY - JOUR
T1 - Integrin modulating factor-1
T2 - A lipid that alters the function of leukocyte integrins
AU - Hermanowski-Vosatka, Anne
AU - Van Strijp, Jos A.G.
AU - Swiggard, William J.
AU - Wright, Samuel D.
PY - 1992/1/24
Y1 - 1992/1/24
N2 - The avidity of integrin CR3 (also known as αMβ2, Mac-1, Mo-1, and CD11b CD18) may be reversibly altered without changes in the number of cell surface receptors. Here we describe a molecule termed integrin modulating factor (IMF-1), which controls CR3 avidity. Addition of IMF-1 to polymorphonuclear leukocytes (PMNs) or to purified CR3 causes enhanced binding of ligand. IMF-1 is not present in resting PMNs, but stimulation of cells results in a transient rise in IMF-1 content that parallels a transient rise in CR3 activity. We suggest that PMNs control adhesivity by controlling synthesis of IMF-1, which then acts as an allosteric activator of leukocyte integrins. IMF-1 is an acidic, amphiphilic molecule of Mr340 ± 16 that does not contain ester, phosphate, amide, sialic acid, or glycosidic or vicinal hydroxyl functionalities, but does contain a carbon-carbon double bond. These results suggest that IMF-1 is an unsaturated fatty acid or an isoprenoid acid.
AB - The avidity of integrin CR3 (also known as αMβ2, Mac-1, Mo-1, and CD11b CD18) may be reversibly altered without changes in the number of cell surface receptors. Here we describe a molecule termed integrin modulating factor (IMF-1), which controls CR3 avidity. Addition of IMF-1 to polymorphonuclear leukocytes (PMNs) or to purified CR3 causes enhanced binding of ligand. IMF-1 is not present in resting PMNs, but stimulation of cells results in a transient rise in IMF-1 content that parallels a transient rise in CR3 activity. We suggest that PMNs control adhesivity by controlling synthesis of IMF-1, which then acts as an allosteric activator of leukocyte integrins. IMF-1 is an acidic, amphiphilic molecule of Mr340 ± 16 that does not contain ester, phosphate, amide, sialic acid, or glycosidic or vicinal hydroxyl functionalities, but does contain a carbon-carbon double bond. These results suggest that IMF-1 is an unsaturated fatty acid or an isoprenoid acid.
UR - http://www.scopus.com/inward/record.url?scp=0026527577&partnerID=8YFLogxK
U2 - 10.1016/0092-8674(92)90475-R
DO - 10.1016/0092-8674(92)90475-R
M3 - Article
C2 - 1733503
AN - SCOPUS:0026527577
SN - 0092-8674
VL - 68
SP - 341
EP - 352
JO - Cell
JF - Cell
IS - 2
ER -