INNO-LiPA DNA line probe assay misidentification of M. smegmatis as Mycobacterium fortuitum complex

Theo van den Broek; Nard G. Janssen; David J. Hetem; Wouter Bekers; Miranda Kamst; Ad C. Fluit; Jakko van Ingen; Johannes G. Kusters; Rob J. Rentenaar

doi:10.1016/j.diagmicrobio.2019.06.010

INNO-LiPA DNA line probe assay misidentification of M. smegmatis as Mycobacterium fortuitum complex

Theo van den Broek^*, Nard G. Janssen, David J. Hetem, Wouter Bekers, Miranda Kamst, Ad C. Fluit, Jakko van Ingen, Johannes G. Kusters, Rob J. Rentenaar

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

2 Citations (Scopus)

Abstract

Seven weeks after being kicked in the face by a cow, a 34-year-old male patient developed a posttraumatic mycobacterial lymphadenitis. A rapidly growing mycobacterial isolate cultured from a surgically drained lymphadenitis pus specimen was identified as Mycobacterium smegmatis by matrix-assisted laser desorption/ionization mass spectrometry and a combination of ITS-, hsp65-, and 16S rRNA-DNA sequence analysis, but as Mycobacterium fortuitum complex using the commercial INNO-LiPA Mycobacteria v2 line probe assay. As it is unclear if the misidentification of this strain is an exception, more research is required.

Original language	English
Article number	114858
Pages (from-to)	114858
Journal	Diagnostic Microbiology and Infectious Disease
Volume	95
Issue number	3
DOIs	https://doi.org/10.1016/j.diagmicrobio.2019.06.010 https://doi.org/10.1016/j.diagmicrobio.2019.06.010
Publication status	Published - 1 Nov 2019

Keywords

Case report
HSP65 sequencing
INNO-LiPA
Mycobacterium fortuitum complex
Mycobacterium smegmatis
Mycobacterium smegmatis/chemistry
Humans
Male
Treatment Outcome
Microbial Sensitivity Tests
Animals
Cattle
Diagnostic Errors
Lymphadenitis/diagnosis
Reagent Kits, Diagnostic
Adult
Mycobacterium Infections, Nontuberculous/diagnosis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Mycobacterium fortuitum/chemistry
Molecular Diagnostic Techniques/methods

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van den Broek, T., Janssen, N. G., Hetem, D. J., Bekers, W., Kamst, M., Fluit, A. C., van Ingen, J., Kusters, J. G., & Rentenaar, R. J. (2019). INNO-LiPA DNA line probe assay misidentification of M. smegmatis as Mycobacterium fortuitum complex. Diagnostic Microbiology and Infectious Disease, 95(3), 114858. Article 114858. https://doi.org/10.1016/j.diagmicrobio.2019.06.010, https://doi.org/10.1016/j.diagmicrobio.2019.06.010

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title = "INNO-LiPA DNA line probe assay misidentification of M. smegmatis as Mycobacterium fortuitum complex",

abstract = "Seven weeks after being kicked in the face by a cow, a 34-year-old male patient developed a posttraumatic mycobacterial lymphadenitis. A rapidly growing mycobacterial isolate cultured from a surgically drained lymphadenitis pus specimen was identified as Mycobacterium smegmatis by matrix-assisted laser desorption/ionization mass spectrometry and a combination of ITS-, hsp65-, and 16S rRNA-DNA sequence analysis, but as Mycobacterium fortuitum complex using the commercial INNO-LiPA Mycobacteria v2 line probe assay. As it is unclear if the misidentification of this strain is an exception, more research is required.",

keywords = "Case report, HSP65 sequencing, INNO-LiPA, Mycobacterium fortuitum complex, Mycobacterium smegmatis, Mycobacterium smegmatis/chemistry, Humans, Male, Treatment Outcome, Microbial Sensitivity Tests, Animals, Cattle, Diagnostic Errors, Lymphadenitis/diagnosis, Reagent Kits, Diagnostic, Adult, Mycobacterium Infections, Nontuberculous/diagnosis, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Mycobacterium fortuitum/chemistry, Molecular Diagnostic Techniques/methods",

author = "\{van den Broek\}, Theo and Janssen, \{Nard G.\} and Hetem, \{David J.\} and Wouter Bekers and Miranda Kamst and Fluit, \{Ad C.\} and \{van Ingen\}, Jakko and Kusters, \{Johannes G.\} and Rentenaar, \{Rob J.\}",

year = "2019",

month = nov,

day = "1",

doi = "10.1016/j.diagmicrobio.2019.06.010",

language = "English",

volume = "95",

pages = "114858",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier",

number = "3",

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van den Broek, T, Janssen, NG, Hetem, DJ, Bekers, W, Kamst, M, Fluit, AC, van Ingen, J, Kusters, JG & Rentenaar, RJ 2019, 'INNO-LiPA DNA line probe assay misidentification of M. smegmatis as Mycobacterium fortuitum complex', Diagnostic Microbiology and Infectious Disease, vol. 95, no. 3, 114858, pp. 114858. https://doi.org/10.1016/j.diagmicrobio.2019.06.010, https://doi.org/10.1016/j.diagmicrobio.2019.06.010

TY - JOUR

T1 - INNO-LiPA DNA line probe assay misidentification of M. smegmatis as Mycobacterium fortuitum complex

AU - van den Broek, Theo

AU - Janssen, Nard G.

AU - Hetem, David J.

AU - Bekers, Wouter

AU - Kamst, Miranda

AU - Fluit, Ad C.

AU - van Ingen, Jakko

AU - Kusters, Johannes G.

AU - Rentenaar, Rob J.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Seven weeks after being kicked in the face by a cow, a 34-year-old male patient developed a posttraumatic mycobacterial lymphadenitis. A rapidly growing mycobacterial isolate cultured from a surgically drained lymphadenitis pus specimen was identified as Mycobacterium smegmatis by matrix-assisted laser desorption/ionization mass spectrometry and a combination of ITS-, hsp65-, and 16S rRNA-DNA sequence analysis, but as Mycobacterium fortuitum complex using the commercial INNO-LiPA Mycobacteria v2 line probe assay. As it is unclear if the misidentification of this strain is an exception, more research is required.

AB - Seven weeks after being kicked in the face by a cow, a 34-year-old male patient developed a posttraumatic mycobacterial lymphadenitis. A rapidly growing mycobacterial isolate cultured from a surgically drained lymphadenitis pus specimen was identified as Mycobacterium smegmatis by matrix-assisted laser desorption/ionization mass spectrometry and a combination of ITS-, hsp65-, and 16S rRNA-DNA sequence analysis, but as Mycobacterium fortuitum complex using the commercial INNO-LiPA Mycobacteria v2 line probe assay. As it is unclear if the misidentification of this strain is an exception, more research is required.

KW - Case report

KW - HSP65 sequencing

KW - INNO-LiPA

KW - Mycobacterium fortuitum complex

KW - Mycobacterium smegmatis

KW - Mycobacterium smegmatis/chemistry

KW - Humans

KW - Male

KW - Treatment Outcome

KW - Microbial Sensitivity Tests

KW - Animals

KW - Cattle

KW - Diagnostic Errors

KW - Lymphadenitis/diagnosis

KW - Reagent Kits, Diagnostic

KW - Adult

KW - Mycobacterium Infections, Nontuberculous/diagnosis

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

KW - Mycobacterium fortuitum/chemistry

KW - Molecular Diagnostic Techniques/methods

UR - https://www.scopus.com/pages/publications/85069718054

U2 - 10.1016/j.diagmicrobio.2019.06.010

DO - 10.1016/j.diagmicrobio.2019.06.010

M3 - Article

C2 - 31358343

AN - SCOPUS:85069718054

SN - 0732-8893

VL - 95

SP - 114858

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 3

M1 - 114858

ER -

INNO-LiPA DNA line probe assay misidentification of M. smegmatis as Mycobacterium fortuitum complex

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Keywords

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