Influence of different conditions on kinetics of tumor necrosis factor alpha release by peripheral blood mononuclear cells after stimulation with Cryptococcus neoformans: A possible explanation for different results

Wendy Chaka, André F.M. Verheul, Andy I.M. Hoepelman*

*Corresponding author for this work

Research output: Contribution to journalShort surveypeer-review

5 Citations (Scopus)

Abstract

In available literature, different kinetics for tumor necrosis factor alpha (TNF-α) release by peripheral blood mononuclear cells are reported upon stimulation with Cryptococcus neoformans. Results in this study showed that shaking cells gives faster kinetics of TNF-α release while large working volumes give lower TNF-α concentrations. Different experimental conditions thus influence kinetics of TNF-α release by phagocytes.

Original languageEnglish
Pages (from-to)792-794
Number of pages3
JournalClinical and Diagnostic Laboratory Immunology
Volume4
Issue number6
Publication statusPublished - 4 Dec 1997

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