Induction of HOX-2 genes in P19 embryocarcinoma cells is dependent on retinoic acid receptor α

Fried Zwartkruis; Frank Kruyt; Paul T. Van Der Saag; Frits Meijlink

doi:10.1006/excr.1993.1108

Induction of HOX-2 genes in P19 embryocarcinoma cells is dependent on retinoic acid receptor α

Fried Zwartkruis
, Frank Kruyt
, Paul T. Van Der Saag
, Frits Meijlink^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

In vertebrate embryos retinoic acid can act as a teratogen and may have a natural function as a morphogen. In both cases the mechanism by which retinoic acid exerts its effect may involve alteration of the level of HOX gene transcription. We have investigated the role of retinoic acid receptor α (RARα) in retinoic acid-dependent induction of HOX-2 gene expression in embryocarcinoma (EC) cells by using the P19 RAC65 EC cell line, which is retinoic acid-resistant due to the expression of a dominant negative RARα. No induction of HOX-2 genes by retinoic acid was seen, while in P19 RAC65 EC cells stably transfected with human RARα under the control of a constitutive promoter HOX-2 gene induction is restored. This shows that RARα is involved in HOX gene induction by retinoic acid.

Original language	English
Pages (from-to)	422-425
Number of pages	4
Journal	Experimental Cell Research
Volume	205
Issue number	2
DOIs	https://doi.org/10.1006/excr.1993.1108
Publication status	Published - 1993
Externally published	Yes

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title = "Induction of HOX-2 genes in P19 embryocarcinoma cells is dependent on retinoic acid receptor α",

abstract = "In vertebrate embryos retinoic acid can act as a teratogen and may have a natural function as a morphogen. In both cases the mechanism by which retinoic acid exerts its effect may involve alteration of the level of HOX gene transcription. We have investigated the role of retinoic acid receptor α (RARα) in retinoic acid-dependent induction of HOX-2 gene expression in embryocarcinoma (EC) cells by using the P19 RAC65 EC cell line, which is retinoic acid-resistant due to the expression of a dominant negative RARα. No induction of HOX-2 genes by retinoic acid was seen, while in P19 RAC65 EC cells stably transfected with human RARα under the control of a constitutive promoter HOX-2 gene induction is restored. This shows that RARα is involved in HOX gene induction by retinoic acid.",

author = "Fried Zwartkruis and Frank Kruyt and \{Van Der Saag\}, \{Paul T.\} and Frits Meijlink",

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doi = "10.1006/excr.1993.1108",

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T1 - Induction of HOX-2 genes in P19 embryocarcinoma cells is dependent on retinoic acid receptor α

AU - Zwartkruis, Fried

AU - Kruyt, Frank

AU - Van Der Saag, Paul T.

AU - Meijlink, Frits

PY - 1993

Y1 - 1993

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AB - In vertebrate embryos retinoic acid can act as a teratogen and may have a natural function as a morphogen. In both cases the mechanism by which retinoic acid exerts its effect may involve alteration of the level of HOX gene transcription. We have investigated the role of retinoic acid receptor α (RARα) in retinoic acid-dependent induction of HOX-2 gene expression in embryocarcinoma (EC) cells by using the P19 RAC65 EC cell line, which is retinoic acid-resistant due to the expression of a dominant negative RARα. No induction of HOX-2 genes by retinoic acid was seen, while in P19 RAC65 EC cells stably transfected with human RARα under the control of a constitutive promoter HOX-2 gene induction is restored. This shows that RARα is involved in HOX gene induction by retinoic acid.

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DO - 10.1006/excr.1993.1108

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ER -

Induction of HOX-2 genes in P19 embryocarcinoma cells is dependent on retinoic acid receptor α

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