TY - JOUR
T1 - Increased microchimerism in peripheral blood of women with systemic lupus erythematosus
T2 - relation with pregnancy
AU - Bos, E. M.J.
AU - Rijnink, E. C.
AU - Zandbergen, M.
AU - Pool, Diaz de
AU - Almekinders, M. M.
AU - Berden, J. H.M.
AU - Bijl, M.
AU - Hagen, E. C.
AU - Kolster-Bijdevaate, C.
AU - Steup-Beekman, G. M.
AU - Wolterbeek, R.
AU - Bloemenkamp, K. W.M.
AU - Baelde, H. J.
AU - Bruijn, J. A.
AU - Bajema, I. M.
AU - Wilhelmus, S.
N1 - Publisher Copyright:
© 2022 Clinical and Experimental Rheumatology S.A.S.. All rights reserved.
PY - 2022/11
Y1 - 2022/11
N2 - Objective We aimed to determine the presence, amount and origin of microchimerism in peripheral blood of pregnant and non-pregnant parous women with systemic lupus erythematosus (SLE) as compared to control subjects. Methods We performed a comparative study in which peripheral blood was drawn from eleven female non-pregnant SLE-patients and 22 control subjects, and from six pregnant SLE-patients and eleven control subjects during gestation and up to six months postpartum. Quantitative PCR for insertion-deletion polymorphisms and null alleles was used to detect microchimerism in peripheral blood mononuclear cells and granulocytes. Results Microchimerism was detected more often in non-pregnant SLE-patients than control subjects (54.4% vs. 13.6%, respectively; p=0.03). When present, the median total number of foetal chimeric cells was 5 gEq/106 in patients and 2.5gEq/106 in control subjects (p=0.048). Microchimerism was mostly foetal in origin; maternal microchimerism was detected in one patient and one control subject. In control subjects, microchimerism was always derived from only one source whereas in 50% of patients it originated from multiple sources. The pregnant patients had a significantly higher median number of foetal chimeric cells in the granulocyte fraction just after delivery than control subjects (7.5 gEq/106 vs. 0 gEq/106, respectively; p=0.02). Conclusion Just after delivery, SLE-patients had more microchimerism than control subjects. Three months post-partum, microchimerism was no longer detectable, only to reappear many years after the last pregnancy, more often and at higher levels in SLE-patients than in control subjects. This suggests that these chimeric cells may originate from non-circulating foetal chimeric stem cells.
AB - Objective We aimed to determine the presence, amount and origin of microchimerism in peripheral blood of pregnant and non-pregnant parous women with systemic lupus erythematosus (SLE) as compared to control subjects. Methods We performed a comparative study in which peripheral blood was drawn from eleven female non-pregnant SLE-patients and 22 control subjects, and from six pregnant SLE-patients and eleven control subjects during gestation and up to six months postpartum. Quantitative PCR for insertion-deletion polymorphisms and null alleles was used to detect microchimerism in peripheral blood mononuclear cells and granulocytes. Results Microchimerism was detected more often in non-pregnant SLE-patients than control subjects (54.4% vs. 13.6%, respectively; p=0.03). When present, the median total number of foetal chimeric cells was 5 gEq/106 in patients and 2.5gEq/106 in control subjects (p=0.048). Microchimerism was mostly foetal in origin; maternal microchimerism was detected in one patient and one control subject. In control subjects, microchimerism was always derived from only one source whereas in 50% of patients it originated from multiple sources. The pregnant patients had a significantly higher median number of foetal chimeric cells in the granulocyte fraction just after delivery than control subjects (7.5 gEq/106 vs. 0 gEq/106, respectively; p=0.02). Conclusion Just after delivery, SLE-patients had more microchimerism than control subjects. Three months post-partum, microchimerism was no longer detectable, only to reappear many years after the last pregnancy, more often and at higher levels in SLE-patients than in control subjects. This suggests that these chimeric cells may originate from non-circulating foetal chimeric stem cells.
KW - chimerism
KW - Key words systemic lupus erythematosus
KW - polymerase chain reaction
KW - pregnancy
UR - http://www.scopus.com/inward/record.url?scp=85141889675&partnerID=8YFLogxK
U2 - 10.55563/clinexprheumatol/75tlgf
DO - 10.55563/clinexprheumatol/75tlgf
M3 - Article
C2 - 35579081
AN - SCOPUS:85141889675
SN - 0392-856X
VL - 40
SP - 2153
EP - 2160
JO - Clinical and Experimental Rheumatology
JF - Clinical and Experimental Rheumatology
IS - 11
ER -