Increased detection of respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses with real-time PCR in samples from patients with respiratory symptoms

A.C. van de Pol; A.M. van Loon; T.F.W. Wolfs; N.J.G. Jansen; M. Nijhuis; E.G. Klein Breteler; R. Schuurman; J.W.A. Rossen

doi:10.1128/JCM.00848-07

Increased detection of respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses with real-time PCR in samples from patients with respiratory symptoms

A.C. van de Pol
, A.M. van Loon
, T.F.W. Wolfs
, N.J.G. Jansen
, M. Nijhuis
, E.G. Klein Breteler
, R. Schuurman
, J.W.A. Rossen

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Respiratory samples (n = 267) from hospitalized patients with respiratory symptoms were tested by real-time PCR, viral culture, and direct immunofluorescence for respiratory syncytial virus, influenza virus, parainfluenza viruses, and adenoviruses. Compared with conventional diagnostic tests, real-time PCR increased the diagnostic yields for these viruses from 24% to 43% and from 3.5% to 36% for children and adults, respectively.

Original language	English
Pages (from-to)	2260-2262
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	45
Issue number	7
DOIs	https://doi.org/10.1128/JCM.00848-07
Publication status	Published - Jul 2007

Keywords

Adenoviridae
Adenoviridae Infections
Adult
Child
Humans
Orthomyxoviridae
Orthomyxoviridae Infections
Paramyxoviridae
Paramyxoviridae Infections
Polymerase Chain Reaction
Respiratory Syncytial Virus Infections
Respiratory Syncytial Viruses

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10.1128/JCM.00848-07

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van de Pol, A. C., van Loon, A. M., Wolfs, T. F. W., Jansen, N. J. G., Nijhuis, M., Klein Breteler, E. G., Schuurman, R., & Rossen, J. W. A. (2007). Increased detection of respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses with real-time PCR in samples from patients with respiratory symptoms. Journal of Clinical Microbiology, 45(7), 2260-2262. https://doi.org/10.1128/JCM.00848-07

@article{f301bb7ffd144cd08fc3eab6f1cde981,

title = "Increased detection of respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses with real-time PCR in samples from patients with respiratory symptoms",

abstract = "Respiratory samples (n = 267) from hospitalized patients with respiratory symptoms were tested by real-time PCR, viral culture, and direct immunofluorescence for respiratory syncytial virus, influenza virus, parainfluenza viruses, and adenoviruses. Compared with conventional diagnostic tests, real-time PCR increased the diagnostic yields for these viruses from 24\% to 43\% and from 3.5\% to 36\% for children and adults, respectively.",

keywords = "Adenoviridae, Adenoviridae Infections, Adult, Child, Humans, Orthomyxoviridae, Orthomyxoviridae Infections, Paramyxoviridae, Paramyxoviridae Infections, Polymerase Chain Reaction, Respiratory Syncytial Virus Infections, Respiratory Syncytial Viruses",

author = "\{van de Pol\}, A.C. and \{van Loon\}, A.M. and T.F.W. Wolfs and N.J.G. Jansen and M. Nijhuis and \{Klein Breteler\}, E.G. and R. Schuurman and J.W.A. Rossen",

year = "2007",

month = jul,

doi = "10.1128/JCM.00848-07",

language = "English",

volume = "45",

pages = "2260--2262",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "7",

}

van de Pol, AC, van Loon, AM, Wolfs, TFW, Jansen, NJG, Nijhuis, M, Klein Breteler, EG, Schuurman, R & Rossen, JWA 2007, 'Increased detection of respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses with real-time PCR in samples from patients with respiratory symptoms', Journal of Clinical Microbiology, vol. 45, no. 7, pp. 2260-2262. https://doi.org/10.1128/JCM.00848-07

Increased detection of respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses with real-time PCR in samples from patients with respiratory symptoms. / van de Pol, A.C.; van Loon, A.M.; Wolfs, T.F.W. et al.
In: Journal of Clinical Microbiology, Vol. 45, No. 7, 07.2007, p. 2260-2262.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Increased detection of respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses with real-time PCR in samples from patients with respiratory symptoms

AU - van de Pol, A.C.

AU - van Loon, A.M.

AU - Wolfs, T.F.W.

AU - Jansen, N.J.G.

AU - Nijhuis, M.

AU - Klein Breteler, E.G.

AU - Schuurman, R.

AU - Rossen, J.W.A.

PY - 2007/7

Y1 - 2007/7

N2 - Respiratory samples (n = 267) from hospitalized patients with respiratory symptoms were tested by real-time PCR, viral culture, and direct immunofluorescence for respiratory syncytial virus, influenza virus, parainfluenza viruses, and adenoviruses. Compared with conventional diagnostic tests, real-time PCR increased the diagnostic yields for these viruses from 24% to 43% and from 3.5% to 36% for children and adults, respectively.

AB - Respiratory samples (n = 267) from hospitalized patients with respiratory symptoms were tested by real-time PCR, viral culture, and direct immunofluorescence for respiratory syncytial virus, influenza virus, parainfluenza viruses, and adenoviruses. Compared with conventional diagnostic tests, real-time PCR increased the diagnostic yields for these viruses from 24% to 43% and from 3.5% to 36% for children and adults, respectively.

KW - Adenoviridae

KW - Adenoviridae Infections

KW - Adult

KW - Child

KW - Humans

KW - Orthomyxoviridae

KW - Orthomyxoviridae Infections

KW - Paramyxoviridae

KW - Paramyxoviridae Infections

KW - Polymerase Chain Reaction

KW - Respiratory Syncytial Virus Infections

KW - Respiratory Syncytial Viruses

U2 - 10.1128/JCM.00848-07

DO - 10.1128/JCM.00848-07

M3 - Article

C2 - 17507513

SN - 0095-1137

VL - 45

SP - 2260

EP - 2262

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 7

ER -

Increased detection of respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses with real-time PCR in samples from patients with respiratory symptoms

Abstract

Keywords

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