In vivo monitoring the changes of interstitial pH and FAD/NADH ratio by fluorescence spectroscopy in healing skin wounds

Michal Mokrý; Peter Gál; Boris Vidinský; Jaroslav Kušnír; Katarína Dubayová; Štefan Mozeš; Ján Sabo

doi:10.1562/2005-09-08-RA-678

In vivo monitoring the changes of interstitial pH and FAD/NADH ratio by fluorescence spectroscopy in healing skin wounds

Michal Mokrý^*, Peter Gál, Boris Vidinský, Jaroslav Kušnír, Katarína Dubayová, Štefan Mozeš, Ján Sabo

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

19 Citations (Scopus)

Abstract

The aim of our study was to evaluate the changes of interstitial pH and flavin adenine dinucleotide (FAD)/reduced nicotinamide adenine dinucleotide (NADH) ratio in healing skin wounds using fluorescence spectroscopy in Sprague Dawley rats. In the experiment, excisional and incisional models of wound healing were used. The florescein as the pH-sensitive probe using excitation spectra (λ_Em = 535 nm) was used for the measurement of pH changes, and synchronous fluorescence spectra (Δλ = 60 nm) for the monitoring of FAD/NADH ratio changes were measured from the surfaces of healing wounds. Increase of interstitial pH and FAD/NADH ratio was recorded during the time interval from the 15th to the 65th minute after surgery. The decrease of pH between the 48th and the 72nd hour after surgery as well as the increase of FAD/NADH ratio between the 72nd and the 96th hour of wound healing were recorded. The results indicate that the use of fluorescence spectroscopy may be considered as a valuable tool for non-invasive in vivo monitoring of selected redox parameters in the early phases of wound healing.

Original language	English
Pages (from-to)	793-797
Number of pages	5
Journal	Photochemistry and Photobiology
Volume	82
Issue number	3
DOIs	https://doi.org/10.1562/2005-09-08-RA-678
Publication status	Published - May 2006
Externally published	Yes

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title = "In vivo monitoring the changes of interstitial pH and FAD/NADH ratio by fluorescence spectroscopy in healing skin wounds",

abstract = "The aim of our study was to evaluate the changes of interstitial pH and flavin adenine dinucleotide (FAD)/reduced nicotinamide adenine dinucleotide (NADH) ratio in healing skin wounds using fluorescence spectroscopy in Sprague Dawley rats. In the experiment, excisional and incisional models of wound healing were used. The florescein as the pH-sensitive probe using excitation spectra (λEm = 535 nm) was used for the measurement of pH changes, and synchronous fluorescence spectra (Δλ = 60 nm) for the monitoring of FAD/NADH ratio changes were measured from the surfaces of healing wounds. Increase of interstitial pH and FAD/NADH ratio was recorded during the time interval from the 15th to the 65th minute after surgery. The decrease of pH between the 48th and the 72nd hour after surgery as well as the increase of FAD/NADH ratio between the 72nd and the 96th hour of wound healing were recorded. The results indicate that the use of fluorescence spectroscopy may be considered as a valuable tool for non-invasive in vivo monitoring of selected redox parameters in the early phases of wound healing.",

author = "Michal Mokr{\'y} and Peter G{\'a}l and Boris Vidinsk{\'y} and Jaroslav Ku{\v s}n{\'i}r and Katar{\'i}na Dubayov{\'a} and {\v S}tefan Moze{\v s} and J{\'a}n Sabo",

year = "2006",

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doi = "10.1562/2005-09-08-RA-678",

language = "English",

volume = "82",

pages = "793--797",

journal = "Photochemistry and Photobiology",

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T1 - In vivo monitoring the changes of interstitial pH and FAD/NADH ratio by fluorescence spectroscopy in healing skin wounds

AU - Mokrý, Michal

AU - Gál, Peter

AU - Vidinský, Boris

AU - Kušnír, Jaroslav

AU - Dubayová, Katarína

AU - Mozeš, Štefan

AU - Sabo, Ján

PY - 2006/5

Y1 - 2006/5

N2 - The aim of our study was to evaluate the changes of interstitial pH and flavin adenine dinucleotide (FAD)/reduced nicotinamide adenine dinucleotide (NADH) ratio in healing skin wounds using fluorescence spectroscopy in Sprague Dawley rats. In the experiment, excisional and incisional models of wound healing were used. The florescein as the pH-sensitive probe using excitation spectra (λEm = 535 nm) was used for the measurement of pH changes, and synchronous fluorescence spectra (Δλ = 60 nm) for the monitoring of FAD/NADH ratio changes were measured from the surfaces of healing wounds. Increase of interstitial pH and FAD/NADH ratio was recorded during the time interval from the 15th to the 65th minute after surgery. The decrease of pH between the 48th and the 72nd hour after surgery as well as the increase of FAD/NADH ratio between the 72nd and the 96th hour of wound healing were recorded. The results indicate that the use of fluorescence spectroscopy may be considered as a valuable tool for non-invasive in vivo monitoring of selected redox parameters in the early phases of wound healing.

AB - The aim of our study was to evaluate the changes of interstitial pH and flavin adenine dinucleotide (FAD)/reduced nicotinamide adenine dinucleotide (NADH) ratio in healing skin wounds using fluorescence spectroscopy in Sprague Dawley rats. In the experiment, excisional and incisional models of wound healing were used. The florescein as the pH-sensitive probe using excitation spectra (λEm = 535 nm) was used for the measurement of pH changes, and synchronous fluorescence spectra (Δλ = 60 nm) for the monitoring of FAD/NADH ratio changes were measured from the surfaces of healing wounds. Increase of interstitial pH and FAD/NADH ratio was recorded during the time interval from the 15th to the 65th minute after surgery. The decrease of pH between the 48th and the 72nd hour after surgery as well as the increase of FAD/NADH ratio between the 72nd and the 96th hour of wound healing were recorded. The results indicate that the use of fluorescence spectroscopy may be considered as a valuable tool for non-invasive in vivo monitoring of selected redox parameters in the early phases of wound healing.

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DO - 10.1562/2005-09-08-RA-678

M3 - Article

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AN - SCOPUS:33745856966

SN - 0031-8655

VL - 82

SP - 793

EP - 797

JO - Photochemistry and Photobiology

JF - Photochemistry and Photobiology

IS - 3

ER -

In vivo monitoring the changes of interstitial pH and FAD/NADH ratio by fluorescence spectroscopy in healing skin wounds

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