TY - JOUR
T1 - In vitro modeling of the neurobiological effects of glucocorticoids
T2 - A review
AU - Bassil, Katherine
AU - Krontira, Anthi C.
AU - Leroy, Thomas
AU - Escoto, Alana I.H.
AU - Snijders, Clara
AU - Pernia, Cameron D.
AU - Pasterkamp, R. Jeroen
AU - de Nijs, Laurence
AU - van den Hove, Daniel
AU - Kenis, Gunter
AU - Boks, Marco P.
AU - Vadodaria, Krishna
AU - Daskalakis, Nikolaos P.
AU - Binder, Elisabeth B.
AU - Rutten, Bart P.F.
N1 - Funding Information:
This work was supported though the awarded VIDI grant of Bart P.F. Rutten, number 91718336, from the Netherlands Scientific Organization (NWO) .
Publisher Copyright:
© 2023 The Authors
PY - 2023/3
Y1 - 2023/3
N2 - Hypothalamic-pituitary adrenal (HPA)axis dysregulation has long been implicated in stress-related disorders such as major depression and post-traumatic stress disorder. Glucocorticoids (GCs) are released from the adrenal glands as a result of HPA-axis activation. The release of GCs is implicated with several neurobiological changes that are associated with negative consequences of chronic stress and the onset and course of psychiatric disorders. Investigating the underlying neurobiological effects of GCs may help to better understand the pathophysiology of stress-related psychiatric disorders. GCs impact a plethora of neuronal processes at the genetic, epigenetic, cellular, and molecular levels. Given the scarcity and difficulty in accessing human brain samples, 2D and 3D in vitro neuronal cultures are becoming increasingly useful in studying GC effects. In this review, we provide an overview of in vitro studies investigating the effects of GCs on key neuronal processes such as proliferation and survival of progenitor cells, neurogenesis, synaptic plasticity, neuronal activity, inflammation, genetic vulnerability, and epigenetic alterations. Finally, we discuss the challenges in the field and offer suggestions for improving the use of in vitro models to investigate GC effects.
AB - Hypothalamic-pituitary adrenal (HPA)axis dysregulation has long been implicated in stress-related disorders such as major depression and post-traumatic stress disorder. Glucocorticoids (GCs) are released from the adrenal glands as a result of HPA-axis activation. The release of GCs is implicated with several neurobiological changes that are associated with negative consequences of chronic stress and the onset and course of psychiatric disorders. Investigating the underlying neurobiological effects of GCs may help to better understand the pathophysiology of stress-related psychiatric disorders. GCs impact a plethora of neuronal processes at the genetic, epigenetic, cellular, and molecular levels. Given the scarcity and difficulty in accessing human brain samples, 2D and 3D in vitro neuronal cultures are becoming increasingly useful in studying GC effects. In this review, we provide an overview of in vitro studies investigating the effects of GCs on key neuronal processes such as proliferation and survival of progenitor cells, neurogenesis, synaptic plasticity, neuronal activity, inflammation, genetic vulnerability, and epigenetic alterations. Finally, we discuss the challenges in the field and offer suggestions for improving the use of in vitro models to investigate GC effects.
KW - Glucocorticoids
KW - In vitro models
KW - Neurobiology
KW - Psychiatry
KW - Stress
KW - Stress disorders
UR - http://www.scopus.com/inward/record.url?scp=85149065696&partnerID=8YFLogxK
U2 - 10.1016/j.ynstr.2023.100530
DO - 10.1016/j.ynstr.2023.100530
M3 - Review article
C2 - 36891528
AN - SCOPUS:85149065696
SN - 2352-2895
VL - 23
JO - Neurobiology of stress
JF - Neurobiology of stress
M1 - 100530
ER -