In vitro antimicrobial susceptibility testing of human Brucella melitensis isolates from Qatar between 2014 - 2015

Anand Deshmukh; Ferry Hagen; Ola Al Sharabasi; Mariamma Abraham; Godwin Wilson; Sanjay Doiphode; Muna Al Maslamani; Jacques F Meis

doi:10.1186/s12866-015-0458-9

In vitro antimicrobial susceptibility testing of human Brucella melitensis isolates from Qatar between 2014 - 2015

Anand Deshmukh^*
, Ferry Hagen
, Ola Al Sharabasi
, Mariamma Abraham
, Godwin Wilson
, Sanjay Doiphode
, Muna Al Maslamani
, Jacques F Meis

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: Brucellosis is one of the most common zoonotic disease affecting humans and animals and is endemic in many parts of the world including the Gulf Cooperation Council region (GCC). The aim of this study was to identify the species and determine the antimicrobial susceptibility pattern of Brucella strains isolated from clinical specimens, from Qatar.

RESULTS: We evaluated 231 Brucella isolates. All isolates were identified as B. melitensis. All the isolates were susceptible to doxycycline, tetracycline, streptomycin, gentamicin, trimethoprim / sulfamethoxazole and ciprofloxacin except rifampicin, where 48 % of the strains showed elevated MICs (>1 mg/L). The rifampicin-resistance related hotspots within the rpoB gene were amplified and sequenced using PCR and no rpoB mutations were found in strains with rifampicin MICs of >2 mg/L.

CONCLUSION: This study identified B. melitensis as the etiological agent of brucellosis in Qatar. No resistant isolates were detected among conventionally used antimicrobial agents.

Original language	English
Pages (from-to)	121
Journal	BMC Microbiology
Volume	15
DOIs	https://doi.org/10.1186/s12866-015-0458-9
Publication status	Published - 16 Jun 2015
Externally published	Yes

Keywords

Anti-Bacterial Agents/pharmacology
Bacterial Proteins/genetics
Brucella melitensis/drug effects
Brucellosis/epidemiology
Doxycycline/pharmacology
Drug Resistance, Bacterial
Gentamicins/pharmacology
Humans
In Vitro Techniques
Microbial Sensitivity Tests
Qatar/epidemiology
Rifampin/pharmacology
Tetracycline/pharmacology

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title = "In vitro antimicrobial susceptibility testing of human Brucella melitensis isolates from Qatar between 2014 - 2015",

abstract = "BACKGROUND: Brucellosis is one of the most common zoonotic disease affecting humans and animals and is endemic in many parts of the world including the Gulf Cooperation Council region (GCC). The aim of this study was to identify the species and determine the antimicrobial susceptibility pattern of Brucella strains isolated from clinical specimens, from Qatar.RESULTS: We evaluated 231 Brucella isolates. All isolates were identified as B. melitensis. All the isolates were susceptible to doxycycline, tetracycline, streptomycin, gentamicin, trimethoprim / sulfamethoxazole and ciprofloxacin except rifampicin, where 48 \% of the strains showed elevated MICs (>1 mg/L). The rifampicin-resistance related hotspots within the rpoB gene were amplified and sequenced using PCR and no rpoB mutations were found in strains with rifampicin MICs of >2 mg/L.CONCLUSION: This study identified B. melitensis as the etiological agent of brucellosis in Qatar. No resistant isolates were detected among conventionally used antimicrobial agents.",

keywords = "Anti-Bacterial Agents/pharmacology, Bacterial Proteins/genetics, Brucella melitensis/drug effects, Brucellosis/epidemiology, Doxycycline/pharmacology, Drug Resistance, Bacterial, Gentamicins/pharmacology, Humans, In Vitro Techniques, Microbial Sensitivity Tests, Qatar/epidemiology, Rifampin/pharmacology, Tetracycline/pharmacology",

author = "Anand Deshmukh and Ferry Hagen and Sharabasi, \{Ola Al\} and Mariamma Abraham and Godwin Wilson and Sanjay Doiphode and Maslamani, \{Muna Al\} and Meis, \{Jacques F\}",

year = "2015",

month = jun,

day = "16",

doi = "10.1186/s12866-015-0458-9",

language = "English",

volume = "15",

pages = "121",

journal = "BMC Microbiology",

issn = "1471-2180",

publisher = "BioMed Central",

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TY - JOUR

T1 - In vitro antimicrobial susceptibility testing of human Brucella melitensis isolates from Qatar between 2014 - 2015

AU - Deshmukh, Anand

AU - Hagen, Ferry

AU - Sharabasi, Ola Al

AU - Abraham, Mariamma

AU - Wilson, Godwin

AU - Doiphode, Sanjay

AU - Maslamani, Muna Al

AU - Meis, Jacques F

PY - 2015/6/16

Y1 - 2015/6/16

N2 - BACKGROUND: Brucellosis is one of the most common zoonotic disease affecting humans and animals and is endemic in many parts of the world including the Gulf Cooperation Council region (GCC). The aim of this study was to identify the species and determine the antimicrobial susceptibility pattern of Brucella strains isolated from clinical specimens, from Qatar.RESULTS: We evaluated 231 Brucella isolates. All isolates were identified as B. melitensis. All the isolates were susceptible to doxycycline, tetracycline, streptomycin, gentamicin, trimethoprim / sulfamethoxazole and ciprofloxacin except rifampicin, where 48 % of the strains showed elevated MICs (>1 mg/L). The rifampicin-resistance related hotspots within the rpoB gene were amplified and sequenced using PCR and no rpoB mutations were found in strains with rifampicin MICs of >2 mg/L.CONCLUSION: This study identified B. melitensis as the etiological agent of brucellosis in Qatar. No resistant isolates were detected among conventionally used antimicrobial agents.

AB - BACKGROUND: Brucellosis is one of the most common zoonotic disease affecting humans and animals and is endemic in many parts of the world including the Gulf Cooperation Council region (GCC). The aim of this study was to identify the species and determine the antimicrobial susceptibility pattern of Brucella strains isolated from clinical specimens, from Qatar.RESULTS: We evaluated 231 Brucella isolates. All isolates were identified as B. melitensis. All the isolates were susceptible to doxycycline, tetracycline, streptomycin, gentamicin, trimethoprim / sulfamethoxazole and ciprofloxacin except rifampicin, where 48 % of the strains showed elevated MICs (>1 mg/L). The rifampicin-resistance related hotspots within the rpoB gene were amplified and sequenced using PCR and no rpoB mutations were found in strains with rifampicin MICs of >2 mg/L.CONCLUSION: This study identified B. melitensis as the etiological agent of brucellosis in Qatar. No resistant isolates were detected among conventionally used antimicrobial agents.

KW - Anti-Bacterial Agents/pharmacology

KW - Bacterial Proteins/genetics

KW - Brucella melitensis/drug effects

KW - Brucellosis/epidemiology

KW - Doxycycline/pharmacology

KW - Drug Resistance, Bacterial

KW - Gentamicins/pharmacology

KW - Humans

KW - In Vitro Techniques

KW - Microbial Sensitivity Tests

KW - Qatar/epidemiology

KW - Rifampin/pharmacology

KW - Tetracycline/pharmacology

U2 - 10.1186/s12866-015-0458-9

DO - 10.1186/s12866-015-0458-9

M3 - Article

C2 - 26073177

SN - 1471-2180

VL - 15

SP - 121

JO - BMC Microbiology

JF - BMC Microbiology

ER -

In vitro antimicrobial susceptibility testing of human Brucella melitensis isolates from Qatar between 2014 - 2015

Abstract

Keywords

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