In situ visualization of antigen-specific T cells in cryopreserved human tissues

Florry A. Vyth-Dreese; Yeung Hyen Kim; Trees A M Dellemijn; Ellen Schrama; John B A G Haanen; Eric Spierings; Els Goulmy

doi:10.1016/j.jim.2005.12.011

In situ visualization of antigen-specific T cells in cryopreserved human tissues

Florry A. Vyth-Dreese, Yeung Hyen Kim, Trees A M Dellemijn, Ellen Schrama, John B A G Haanen, Eric Spierings^*, Els Goulmy

^*Corresponding author for this work

Infection & Immunity

Research output: Contribution to journal › Article › Academic › peer-review

9 Citations (Scopus)

Abstract

Tetrameric MHC/peptide complexes are important tools for analyzing antigen-specific T cells. The in situ use of tetrameric MHC/peptide complexes in viable tissue sections has several shortcomings: it does not allow the execution of multiple analyses on one single biopsy, the storage of the biopsies, and the co-staining of the tetramer-positive cells for various intracellular molecules. We have developed a novel approach using overnight pre-labeling of viable human tissues with MHC/peptide tetramers, followed by cryopreservation and labeling of the cryosections. The visualization of antigen-specific T cells, combined with detection of other membrane, cytoplasmic, or nuclear markers is now feasible.

Original language	English
Pages (from-to)	78-85
Number of pages	8
Journal	Journal of Immunological Methods
Volume	310
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jim.2005.12.011
Publication status	Published - 20 Mar 2006

Keywords

CD8-positive T-lymphocytes/immunology
Ex vivo
Fluorescent Dyes
Humans
In situ
Major Histocompatibility antigens class I
Minor Histocompatibility antigens
Staining and labeling methods

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10.1016/j.jim.2005.12.011

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title = "In situ visualization of antigen-specific T cells in cryopreserved human tissues",

abstract = "Tetrameric MHC/peptide complexes are important tools for analyzing antigen-specific T cells. The in situ use of tetrameric MHC/peptide complexes in viable tissue sections has several shortcomings: it does not allow the execution of multiple analyses on one single biopsy, the storage of the biopsies, and the co-staining of the tetramer-positive cells for various intracellular molecules. We have developed a novel approach using overnight pre-labeling of viable human tissues with MHC/peptide tetramers, followed by cryopreservation and labeling of the cryosections. The visualization of antigen-specific T cells, combined with detection of other membrane, cytoplasmic, or nuclear markers is now feasible.",

keywords = "CD8-positive T-lymphocytes/immunology, Ex vivo, Fluorescent Dyes, Humans, In situ, Major Histocompatibility antigens class I, Minor Histocompatibility antigens, Staining and labeling methods",

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T1 - In situ visualization of antigen-specific T cells in cryopreserved human tissues

AU - Vyth-Dreese, Florry A.

AU - Kim, Yeung Hyen

AU - Dellemijn, Trees A M

AU - Schrama, Ellen

AU - Haanen, John B A G

AU - Spierings, Eric

AU - Goulmy, Els

PY - 2006/3/20

Y1 - 2006/3/20

N2 - Tetrameric MHC/peptide complexes are important tools for analyzing antigen-specific T cells. The in situ use of tetrameric MHC/peptide complexes in viable tissue sections has several shortcomings: it does not allow the execution of multiple analyses on one single biopsy, the storage of the biopsies, and the co-staining of the tetramer-positive cells for various intracellular molecules. We have developed a novel approach using overnight pre-labeling of viable human tissues with MHC/peptide tetramers, followed by cryopreservation and labeling of the cryosections. The visualization of antigen-specific T cells, combined with detection of other membrane, cytoplasmic, or nuclear markers is now feasible.

AB - Tetrameric MHC/peptide complexes are important tools for analyzing antigen-specific T cells. The in situ use of tetrameric MHC/peptide complexes in viable tissue sections has several shortcomings: it does not allow the execution of multiple analyses on one single biopsy, the storage of the biopsies, and the co-staining of the tetramer-positive cells for various intracellular molecules. We have developed a novel approach using overnight pre-labeling of viable human tissues with MHC/peptide tetramers, followed by cryopreservation and labeling of the cryosections. The visualization of antigen-specific T cells, combined with detection of other membrane, cytoplasmic, or nuclear markers is now feasible.

KW - CD8-positive T-lymphocytes/immunology

KW - Ex vivo

KW - Fluorescent Dyes

KW - Humans

KW - In situ

KW - Major Histocompatibility antigens class I

KW - Minor Histocompatibility antigens

KW - Staining and labeling methods

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EP - 85

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1-2

ER -

In situ visualization of antigen-specific T cells in cryopreserved human tissues

Abstract

Keywords

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