Impact of cleavage-stage embryo biopsy in view of PGD on human blastocyst implantation: a prospective cohort of single embryo transfers

A De Vos; C Staessen; M De Rycke; W Verpoest; P Haentjens; P Devroey; I Liebaers; H Van de Velde

doi:10.1093/humrep/dep251

Impact of cleavage-stage embryo biopsy in view of PGD on human blastocyst implantation: a prospective cohort of single embryo transfers

A De Vos^*, C Staessen, M De Rycke, W Verpoest, P Haentjens, P Devroey, I Liebaers, H Van de Velde

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: Human embryo biopsy is performed for preimplantation genetic diagnosis (PGD). The impact of 1- or 2-cell removal at cleavage-stage on future embryo development and implantation capacity is highly debated.

METHODS: In order to explore this issue further, a cohort of Day 5 single embryo transfers was analysed prospectively for embryological and clinical outcome. All transferred embryos resulted from 8-cell embryos on Day 3, from which subsequently either one cell (group I, n = 182) or two cells (group II, n = 259) were removed, or on which no invasion by means of embryo biopsy was performed (group III, control group, n = 702). RESULTS Blastocyst formation was significantly better in group III compared with group II, and similar to group I. Group I and group II did not differ in Day 3 nor in Day 5 embryo development. The overall live birth rate was significantly higher in group I (37.4%, CI 29.0-47.4%) than in group II (22.4%, CI 17.0-28.9%), and comparable to the reference ICSI population (35.0%, CI 30.8-39.7%).

CONCLUSIONS: The clinical outcome of 1-cell biopsy was significantly better than that of 2-cell biopsy, even when adjusted for availability of genetically transferable embryos.

Original language	English
Pages (from-to)	2988-96
Number of pages	9
Journal	Human reproduction (Oxford, England)
Volume	24
Issue number	12
DOIs	https://doi.org/10.1093/humrep/dep251
Publication status	Published - Dec 2009
Externally published	Yes

Keywords

Adult
Biopsy/adverse effects
Cleavage Stage, Ovum
Embryo Implantation
Female
Humans
Pregnancy
Pregnancy Outcome
Preimplantation Diagnosis/methods
Prospective Studies
Single Embryo Transfer
Statistics as Topic
Young Adult

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title = "Impact of cleavage-stage embryo biopsy in view of PGD on human blastocyst implantation: a prospective cohort of single embryo transfers",

abstract = "BACKGROUND: Human embryo biopsy is performed for preimplantation genetic diagnosis (PGD). The impact of 1- or 2-cell removal at cleavage-stage on future embryo development and implantation capacity is highly debated.METHODS: In order to explore this issue further, a cohort of Day 5 single embryo transfers was analysed prospectively for embryological and clinical outcome. All transferred embryos resulted from 8-cell embryos on Day 3, from which subsequently either one cell (group I, n = 182) or two cells (group II, n = 259) were removed, or on which no invasion by means of embryo biopsy was performed (group III, control group, n = 702). RESULTS Blastocyst formation was significantly better in group III compared with group II, and similar to group I. Group I and group II did not differ in Day 3 nor in Day 5 embryo development. The overall live birth rate was significantly higher in group I (37.4%, CI 29.0-47.4%) than in group II (22.4%, CI 17.0-28.9%), and comparable to the reference ICSI population (35.0%, CI 30.8-39.7%).CONCLUSIONS: The clinical outcome of 1-cell biopsy was significantly better than that of 2-cell biopsy, even when adjusted for availability of genetically transferable embryos.",

keywords = "Adult, Biopsy/adverse effects, Cleavage Stage, Ovum, Embryo Implantation, Female, Humans, Pregnancy, Pregnancy Outcome, Preimplantation Diagnosis/methods, Prospective Studies, Single Embryo Transfer, Statistics as Topic, Young Adult",

author = "{De Vos}, A and C Staessen and {De Rycke}, M and W Verpoest and P Haentjens and P Devroey and I Liebaers and {Van de Velde}, H",

year = "2009",

month = dec,

doi = "10.1093/humrep/dep251",

language = "English",

volume = "24",

pages = "2988--96",

journal = "Human reproduction (Oxford, England)",

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publisher = "Oxford University Press",

number = "12",

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TY - JOUR

T1 - Impact of cleavage-stage embryo biopsy in view of PGD on human blastocyst implantation

T2 - a prospective cohort of single embryo transfers

AU - De Vos, A

AU - Staessen, C

AU - De Rycke, M

AU - Verpoest, W

AU - Haentjens, P

AU - Devroey, P

AU - Liebaers, I

AU - Van de Velde, H

PY - 2009/12

Y1 - 2009/12

N2 - BACKGROUND: Human embryo biopsy is performed for preimplantation genetic diagnosis (PGD). The impact of 1- or 2-cell removal at cleavage-stage on future embryo development and implantation capacity is highly debated.METHODS: In order to explore this issue further, a cohort of Day 5 single embryo transfers was analysed prospectively for embryological and clinical outcome. All transferred embryos resulted from 8-cell embryos on Day 3, from which subsequently either one cell (group I, n = 182) or two cells (group II, n = 259) were removed, or on which no invasion by means of embryo biopsy was performed (group III, control group, n = 702). RESULTS Blastocyst formation was significantly better in group III compared with group II, and similar to group I. Group I and group II did not differ in Day 3 nor in Day 5 embryo development. The overall live birth rate was significantly higher in group I (37.4%, CI 29.0-47.4%) than in group II (22.4%, CI 17.0-28.9%), and comparable to the reference ICSI population (35.0%, CI 30.8-39.7%).CONCLUSIONS: The clinical outcome of 1-cell biopsy was significantly better than that of 2-cell biopsy, even when adjusted for availability of genetically transferable embryos.

AB - BACKGROUND: Human embryo biopsy is performed for preimplantation genetic diagnosis (PGD). The impact of 1- or 2-cell removal at cleavage-stage on future embryo development and implantation capacity is highly debated.METHODS: In order to explore this issue further, a cohort of Day 5 single embryo transfers was analysed prospectively for embryological and clinical outcome. All transferred embryos resulted from 8-cell embryos on Day 3, from which subsequently either one cell (group I, n = 182) or two cells (group II, n = 259) were removed, or on which no invasion by means of embryo biopsy was performed (group III, control group, n = 702). RESULTS Blastocyst formation was significantly better in group III compared with group II, and similar to group I. Group I and group II did not differ in Day 3 nor in Day 5 embryo development. The overall live birth rate was significantly higher in group I (37.4%, CI 29.0-47.4%) than in group II (22.4%, CI 17.0-28.9%), and comparable to the reference ICSI population (35.0%, CI 30.8-39.7%).CONCLUSIONS: The clinical outcome of 1-cell biopsy was significantly better than that of 2-cell biopsy, even when adjusted for availability of genetically transferable embryos.

KW - Adult

KW - Biopsy/adverse effects

KW - Cleavage Stage, Ovum

KW - Embryo Implantation

KW - Female

KW - Humans

KW - Pregnancy

KW - Pregnancy Outcome

KW - Preimplantation Diagnosis/methods

KW - Prospective Studies

KW - Single Embryo Transfer

KW - Statistics as Topic

KW - Young Adult

U2 - 10.1093/humrep/dep251

DO - 10.1093/humrep/dep251

M3 - Article

C2 - 19773223

SN - 0268-1161

VL - 24

SP - 2988

EP - 2996

JO - Human reproduction (Oxford, England)

JF - Human reproduction (Oxford, England)

IS - 12

ER -

Impact of cleavage-stage embryo biopsy in view of PGD on human blastocyst implantation: a prospective cohort of single embryo transfers

Abstract

Keywords

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