IgE binding to peanut components by four different techniques: Ara h 2 is the most relevant in peanut allergic children and adults

R.J.B. Klemans, X Liu, A.C. Knulst, M.J. Knol, F.H.J. Gmelig Meyling, H.P.E. Borst, S.G.M.A. Pasmans, E.F. Knol

Research output: Contribution to journalArticleAcademicpeer-review


BACKGROUND: Several studies have analysed the diagnostic value of specific IgE (sIgE) for individual peanut allergens. However, little is known about the concordance between different techniques available in both children and adults.

OBJECTIVE: To evaluate the value of individual peanut allergens by different techniques, i.e. multi-plexed microarray, single-plexed IgE assay, skin prick test (SPT) and immunoblot in both peanut allergic adults and children.

METHODS: Sensitization patterns to peanut allergens Ara h 1, 2, 3, and 8 were evaluated using four different techniques: multi-plexed microarray immunoassay, single-plexed IgE assay, SPT and immunoblot. Twenty-two peanut allergic adults and 15 children scored on clinical severity according to double-blind, placebo-controlled food challenges and 27 atopic control patients were included.

RESULTS: Comparable sensitivity values were found between all four techniques in adults, with the highest sensitivity for Ara h 2 (76.2-95.5%, compared to 100% with all techniques in children). The multi-plexed assay to Ara h 1 (93.3%) demonstrated a higher sensitivity compared with the other three techniques (P = 0.04) in children, but absolute values were perfectly correlated. There were no differences between adults and children. The area under the receiver operating characteristic curve (AUC) of sIgE to Ara h 1 was higher with the multi-plexed assay compared with the single-plexed assay (0.91 vs. 0.75). In adults, sIgE to Ara h 1, 2, and 3 was correlated with clinical severity. No such correlation was found in children.

CONCLUSION AND CLINICAL RELEVANCE: In conclusion, the single- and multi-plexed assay, SPT and immunoblot perform equally in both peanut allergic adults and children, with Ara h 2 being most often recognized with all techniques. Specific IgE to Ara h 1, 2, and 3 in adults was correlated with severity.

Original languageEnglish
Pages (from-to)967-974
Number of pages8
JournalClinical and Experimental Allergy
Issue number8
Publication statusPublished - Aug 2013


  • 2S Albumins, Plant
  • Adult
  • Age Factors
  • Antigens, Plant
  • Arachis
  • Child
  • Child, Preschool
  • Female
  • Glycoproteins
  • Humans
  • Immune Tolerance
  • Immunoassay
  • Immunoglobulin E
  • Male
  • Peanut Hypersensitivity
  • Plant Proteins
  • Seed Storage Proteins
  • Sensitivity and Specificity
  • Severity of Illness Index


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