Abstract
A fundamental hallmark of cancer is progression to metastasis and the growth of breast cancer metastases in lung, bone, liver and/or brain causes fatal complications. Unfortunately, the cellular and biochemical mechanisms of the metastatic process remain ill-defined. Recent application of intravital multiphoton microscopy (MP-IVM) to image fluorescently labeled cells in mouse models of cancer has allowed dynamic observation of this multi-step process at the cellular and subcellular levels. In this article, we discuss the use of MP-IVM in studies of breast cancer metastasis, as well as surgical techniques for exposing tumors prior to imaging. We also describe a versatile multiphoton microscope for imaging tumor-stroma interactions.
| Translated title of the contribution | If you don't look, you won't see: intravital multiphoton imaging of primary and metastatic breast cancer. |
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| Original language | Undefined/Unknown |
| Pages (from-to) | 125-9 |
| Number of pages | 5 |
| Journal | Journal of Mammary Gland Biology and Neoplasia |
| Volume | 17 |
| Issue number | 2 |
| Publication status | Published - 2012 |