Identifying bacterial immune evasion proteins using phage display

Cindy Fevre, Lisette Scheepmaker, Pieter Jan Haas*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

1 Citation (Scopus)


Methods aimed at identification of immune evasion proteins are mainly rely on in silico prediction of sequence, structural homology to known evasion proteins or use a proteomics driven approach. Although proven successful these methods are limited by a low efficiency and or lack of functional identification. Here we describe a high- throughput genomic strategy to functionally identify bacterial immune evasion proteins using phage display technology. Genomic bacterial DNA is randomly fragmented and ligated into a phage display vector that is used to create a phage display library expressing bacterial secreted and membrane bound proteins. This library is used to select displayed bacterial secretome proteins that interact with host immune components.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages19
Publication statusPublished - 1 Jan 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Functional identification
  • High-throughput
  • Immune evasion
  • Phage display
  • Secretome


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