TY - JOUR
T1 - Identification of the missing links in prokaryotic pentose oxidation pathways
T2 - Evidence for enzyme recruitment
AU - Brouns, Stan J.J.
AU - Walther, Jasper
AU - Snijders, Ambrosius P.L.
AU - Van De Werken, Harmen J.G.
AU - Willemen, Hanneke L.D.M.
AU - Worm, Petra
AU - De Vos, Marjon G.J.
AU - Andersson, Anders
AU - Lundgren, Magnus
AU - Mazon, Hortense F.M.
AU - Van Den Heuvel, Robert H.H.
AU - Nilsson, Peter
AU - Salmon, Laurent
AU - De Vos, Willem M.
AU - Wright, Phillip C.
AU - Bernander, Rolf
AU - Van Der Oost, John
PY - 2006/9/15
Y1 - 2006/9/15
N2 - The pentose metabolism of Archaea is largely unknown. Here, we have employed an integrated genomics approach including DNA microarray and proteomics analyses to elucidate the catabolic pathway for D-arabinose in Sulfolobus solfataricus. During growth on this sugar, a small set of genes appeared to be differentially expressed compared with growth on D-glucose. These genes were heterologously overexpressed in Escherichia coli, and the recombinant proteins were purified and biochemically studied. This showed that D-arabinose is oxidized to 2-oxoglutarate by the consecutive action of a number of previously uncharacterized enzymes, including a D-arabinose dehydrogenase, a D-arabinonate dehydratase, a novel 2-keto-3-deoxy-D-arabinonate dehydratase, and a 2,5-dioxopentanoate dehydrogenase. Promoter analysis of these genes revealed a palindromic sequence upstream of the TATA box, which is likely to be involved in their concerted transcriptional control. Integration of the obtained biochemical data with genomic context analysis strongly suggests the occurrence of pentose oxidation pathways in both Archaea and Bacteria, and predicts the involvement of additional enzyme components. Moreover, it revealed striking genetic similarities between the catabolic pathways for pentoses, hexaric acids, and hydroxyproline degradation, which support the theory of metabolic pathway genesis by enzyme recruitment.
AB - The pentose metabolism of Archaea is largely unknown. Here, we have employed an integrated genomics approach including DNA microarray and proteomics analyses to elucidate the catabolic pathway for D-arabinose in Sulfolobus solfataricus. During growth on this sugar, a small set of genes appeared to be differentially expressed compared with growth on D-glucose. These genes were heterologously overexpressed in Escherichia coli, and the recombinant proteins were purified and biochemically studied. This showed that D-arabinose is oxidized to 2-oxoglutarate by the consecutive action of a number of previously uncharacterized enzymes, including a D-arabinose dehydrogenase, a D-arabinonate dehydratase, a novel 2-keto-3-deoxy-D-arabinonate dehydratase, and a 2,5-dioxopentanoate dehydrogenase. Promoter analysis of these genes revealed a palindromic sequence upstream of the TATA box, which is likely to be involved in their concerted transcriptional control. Integration of the obtained biochemical data with genomic context analysis strongly suggests the occurrence of pentose oxidation pathways in both Archaea and Bacteria, and predicts the involvement of additional enzyme components. Moreover, it revealed striking genetic similarities between the catabolic pathways for pentoses, hexaric acids, and hydroxyproline degradation, which support the theory of metabolic pathway genesis by enzyme recruitment.
UR - http://www.scopus.com/inward/record.url?scp=33748754005&partnerID=8YFLogxK
U2 - 10.1074/jbc.M605549200
DO - 10.1074/jbc.M605549200
M3 - Article
C2 - 16849334
AN - SCOPUS:33748754005
SN - 0021-9258
VL - 281
SP - 27378
EP - 27388
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 37
ER -