Human platelet antigen‐1 (Zw) typing of fetuses by analysis of polymerase chain reaction‐amplified genomic DNA from amniocytes

S. Simsek; G. C.L.M. Christiaens; H. H.H. Kanhai; J. R. Beekhuis; P. M.M. Bleeker; A. B.J. Vlekke; R. Goldschmeding; A. E.G. Kr. von dem Borne

doi:10.1111/j.1365-3148.1994.tb00238.x

Human platelet antigen‐1 (Zw) typing of fetuses by analysis of polymerase chain reaction‐amplified genomic DNA from amniocytes

S. Simsek
, G. C.L.M. Christiaens
, H. H.H. Kanhai
, J. R. Beekhuis
, P. M.M. Bleeker
, A. B.J. Vlekke
, R. Goldschmeding
, A. E.G. Kr. von dem Borne^*

^*Corresponding author for this work

Birth Center Obstetrics - Medical

Research output: Contribution to journal › Article › Academic › peer-review

15 Citations (Scopus)

Abstract

SUMMARY. Prenatal typing for the human platelet antigens‐1 (HPA) permits identification of a fetus at risk for neonatal alloimmune thrombocytopenia (NAITP) in cases of HPA‐1 incompatibility in which the father is heterozygous for the HPA‐la antigen. Diagnostic cordocentesis and phenotyping of the fetal platelets are used for this purpose. We applied allele‐specific restriction enzyme analysis on polymerase chain reaction (PCR)‐amplified DNA purified from amniocytes. This assay allows early second trimester typing for HPA‐1 alleles. We were able to determine the genotype of three fetuses at risk. Iatrogenic fetal loss is lower with amniocentesis than with cordocentesis. Therefore, this technique is a welcome addition to the antenatal management of NAITP.

Original language	English
Pages (from-to)	15-19
Number of pages	5
Journal	Transfusion Medicine
Volume	4
Issue number	1
DOIs	https://doi.org/10.1111/j.1365-3148.1994.tb00238.x
Publication status	Published - Mar 1994

Keywords

allele‐specific restriction enzyme analysis
DNA polymorphism
HPA‐1 (Zw/P1)
neonatal alloimmune thrombocytopenia
platelets
prenatal diagnosis

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10.1111/j.1365-3148.1994.tb00238.x

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Simsek, S., Christiaens, G. C. L. M., Kanhai, H. H. H., Beekhuis, J. R., Bleeker, P. M. M., Vlekke, A. B. J., Goldschmeding, R., & Kr. von dem Borne, A. E. G. (1994). Human platelet antigen‐1 (Zw) typing of fetuses by analysis of polymerase chain reaction‐amplified genomic DNA from amniocytes. Transfusion Medicine, 4(1), 15-19. https://doi.org/10.1111/j.1365-3148.1994.tb00238.x

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title = "Human platelet antigen‐1 (Zw) typing of fetuses by analysis of polymerase chain reaction‐amplified genomic DNA from amniocytes",

abstract = "SUMMARY. Prenatal typing for the human platelet antigens‐1 (HPA) permits identification of a fetus at risk for neonatal alloimmune thrombocytopenia (NAITP) in cases of HPA‐1 incompatibility in which the father is heterozygous for the HPA‐la antigen. Diagnostic cordocentesis and phenotyping of the fetal platelets are used for this purpose. We applied allele‐specific restriction enzyme analysis on polymerase chain reaction (PCR)‐amplified DNA purified from amniocytes. This assay allows early second trimester typing for HPA‐1 alleles. We were able to determine the genotype of three fetuses at risk. Iatrogenic fetal loss is lower with amniocentesis than with cordocentesis. Therefore, this technique is a welcome addition to the antenatal management of NAITP.",

keywords = "allele‐specific restriction enzyme analysis, DNA polymorphism, HPA‐1 (Zw/P1), neonatal alloimmune thrombocytopenia, platelets, prenatal diagnosis",

author = "S. Simsek and Christiaens, \{G. C.L.M.\} and Kanhai, \{H. H.H.\} and Beekhuis, \{J. R.\} and Bleeker, \{P. M.M.\} and Vlekke, \{A. B.J.\} and R. Goldschmeding and \{Kr. von dem Borne\}, \{A. E.G.\}",

year = "1994",

month = mar,

doi = "10.1111/j.1365-3148.1994.tb00238.x",

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AU - Simsek, S.

AU - Christiaens, G. C.L.M.

AU - Kanhai, H. H.H.

AU - Beekhuis, J. R.

AU - Bleeker, P. M.M.

AU - Vlekke, A. B.J.

AU - Goldschmeding, R.

AU - Kr. von dem Borne, A. E.G.

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N2 - SUMMARY. Prenatal typing for the human platelet antigens‐1 (HPA) permits identification of a fetus at risk for neonatal alloimmune thrombocytopenia (NAITP) in cases of HPA‐1 incompatibility in which the father is heterozygous for the HPA‐la antigen. Diagnostic cordocentesis and phenotyping of the fetal platelets are used for this purpose. We applied allele‐specific restriction enzyme analysis on polymerase chain reaction (PCR)‐amplified DNA purified from amniocytes. This assay allows early second trimester typing for HPA‐1 alleles. We were able to determine the genotype of three fetuses at risk. Iatrogenic fetal loss is lower with amniocentesis than with cordocentesis. Therefore, this technique is a welcome addition to the antenatal management of NAITP.

AB - SUMMARY. Prenatal typing for the human platelet antigens‐1 (HPA) permits identification of a fetus at risk for neonatal alloimmune thrombocytopenia (NAITP) in cases of HPA‐1 incompatibility in which the father is heterozygous for the HPA‐la antigen. Diagnostic cordocentesis and phenotyping of the fetal platelets are used for this purpose. We applied allele‐specific restriction enzyme analysis on polymerase chain reaction (PCR)‐amplified DNA purified from amniocytes. This assay allows early second trimester typing for HPA‐1 alleles. We were able to determine the genotype of three fetuses at risk. Iatrogenic fetal loss is lower with amniocentesis than with cordocentesis. Therefore, this technique is a welcome addition to the antenatal management of NAITP.

KW - allele‐specific restriction enzyme analysis

KW - DNA polymorphism

KW - HPA‐1 (Zw/P1)

KW - neonatal alloimmune thrombocytopenia

KW - platelets

KW - prenatal diagnosis

UR - https://www.scopus.com/pages/publications/0028387211

U2 - 10.1111/j.1365-3148.1994.tb00238.x

DO - 10.1111/j.1365-3148.1994.tb00238.x

M3 - Article

C2 - 7516786

AN - SCOPUS:0028387211

SN - 0958-7578

VL - 4

SP - 15

EP - 19

JO - Transfusion Medicine

JF - Transfusion Medicine

IS - 1

ER -

Human platelet antigen‐1 (Zw) typing of fetuses by analysis of polymerase chain reaction‐amplified genomic DNA from amniocytes

Abstract

Keywords

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