Human Papillomavirus Antibodies and Future Risk of Anogenital Cancer: A Nested Case-Control Study in the European Prospective Investigation Into Cancer and Nutrition Study

Aimee R. Kreimer*, Paul Brennan, Krystle A. Lang Kuhs, Tim Waterboer, Gary Clifford, Silvia Franceschi, Angelika Michel, Martina Willhauck-Fleckenstein, Elio Riboli, Xavier Castellsague, Allan Hildesheim, Renee Turzanski Fortner, Rudolf Kaaks, Domenico Palli, Ingrid Ljuslinder, Salvatore Panico, Francoise Clavel-Chapelon, Marie-Christine Boutron-Ruault, Sylvie Mesrine, Antonia TrichopoulouPagona Lagiou, Dimitrios Trichopoulos, Petra H. Peeters, Amanda J. Cross, H. Bas Bueno-de-Mesquita, Paolo Vineis, Nerea Larranaga, Valeria Pala, Maria-Jose Sanchez, Carmen Navarro, Aurelio Barricarte, Rosario Tumino, Kay-Tee Khaw, Nicholas Wareham, Heiner Boeing, Annika Steffen, Ruth C. Travis, J. Ramon Quiros, Elisabete Weiderpass, Michael Pawlita, Mattias Johansson

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Purpose

Human papillomavirus (HPV) type 16 (HPV16) causes cancer at several anatomic sites. In the European Prospective Investigation Into Cancer and Nutrition study, HPV16 E6 seropositivity was present more than 10 years before oropharyngeal cancer diagnosis and was nearly absent in controls. The current study sought to evaluate the extent to which HPV16 E6 antibodies are present before diagnosis of anogenital cancers within the same cohort.

Methods

Four hundred incident anogenital cancers (273 cervical, 24 anal, 67 vulvar, 12 vaginal, and 24 penile cancers) with prediagnostic blood samples (collected on average 3 and 8 years before diagnosis for cervix and noncervix cancers, respectively) and 718 matched controls were included. Plasma was analyzed for antibodies against HPV16 E6 and multiple other HPV proteins and genotypes and evaluated in relation to risk using unconditional logistic regression.

Results

HPV16 E6 seropositivity was present in 29.2% of individuals (seven of 24 individuals) who later developed anal cancer compared with 0.6% of controls (four of 718 controls) who remained cancer free (odds ratio [OR], 75.9; 95% CI, 17.9 to 321). HPV16 E6 seropositivity was less common for cancers of the cervix (3.3%), vagina (8.3%), vulva (1.5%), and penis (8.3%). No associations were seen for non-type 16 HPV E6 antibodies, apart from anti-HPV58 E6 and anal cancer (OR, 6.8; 95% CI, 1.4 to 33.1). HPV16 E6 seropositivity tended to increase in blood samples drawn closer in time to cancer diagnosis.

Conclusion

HPV16 E6 seropositivity is relatively common before diagnosis of anal cancer but rare for other HPV-related anogenital cancers. (C) 2015 by American Society of Clinical Oncology

Original languageEnglish
Pages (from-to)877-+
Number of pages10
JournalJournal of Clinical Oncology
Volume33
Issue number8
DOIs
Publication statusPublished - 10 Mar 2015

Keywords

  • INVASIVE CERVICAL-CANCER
  • OROPHARYNGEAL CANCER
  • GENOTYPE ATTRIBUTION
  • EARLY PROTEINS
  • NECK-CANCER
  • CARCINOMA
  • MARKERS
  • PREVALENCE
  • WORLDWIDE
  • COHORT

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