TY - JOUR
T1 - Histological and molecular characterization of bone integrity in osteogenesis imperfecta
T2 - a case series across genetic subtypes
AU - Wu, Zhiming
AU - den Haan, Suzanne
AU - King, Helen E
AU - Nijhuis, Wouter H
AU - Weinans, Harrie
AU - Spaans, Anne J
AU - Sakkers, Ralph
AU - Warmink, Kelly
N1 - Publisher Copyright:
© The Author(s) 2026. Published by Oxford University Press on behalf of the American Society for Bone and Mineral Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
PY - 2026/8
Y1 - 2026/8
N2 - Osteogenesis imperfecta (OI) is a genetically heterogeneous skeletal disorder characterized by bone fragility and variable clinical severity. However, how molecular defects translate into alterations of bone microstructure and composition across OI subtypes remains incompletely understood. In this case series, we systematically evaluated cortical bone integrity in patients with OI types 1, 3, 4, 6, 8, and 14 using histological and molecular approaches, including Raman spectroscopy, and compared findings with non-OI controls. Histological analyses revealed case-specific disruption of bone architecture across OI cases, where the severity of bone disorganization increased progressively from OI type 1 to types 3, 6, 8, and 14. In addition osteocyte lacunar area (Ot.Lc.Ar) was increased specifically in OI subtypes 1, 6, 8, and 14 bones, while osteocyte lacunar appearance was heterogeneous in size, shape, alignment, and spatial distribution in OI types 3, 6, 8, and 14, underscoring the case-specific alterations. Consistently, polarized light microscopy demonstrated increased green birefringence under polarized light microscopy in OI types 1 and 14 and reduced lamellar thickness in OI types 1, 6, and 8. At the molecular level, Raman spectroscopic analyses showed reduced mineral and organic matrix signals in OI bone, specifically OI type 3, indicating compromised mineralization and altered bone matrix composition. Together, these findings illustrate the potential that OI bone phenotype illustrates potential subtype-specific trends in bone microarchitecture, collagen disorganization, impaired lamellar bone formation, and deficits in bone mineral and matrix composition. This integrative analysis links genetic defects in collagen-related and non-collagen genes to multiscale alterations in bone tissue, providing mechanistic insight into OI pathophysiology and highlighting potential structural targets for individualized therapeutic strategies.
AB - Osteogenesis imperfecta (OI) is a genetically heterogeneous skeletal disorder characterized by bone fragility and variable clinical severity. However, how molecular defects translate into alterations of bone microstructure and composition across OI subtypes remains incompletely understood. In this case series, we systematically evaluated cortical bone integrity in patients with OI types 1, 3, 4, 6, 8, and 14 using histological and molecular approaches, including Raman spectroscopy, and compared findings with non-OI controls. Histological analyses revealed case-specific disruption of bone architecture across OI cases, where the severity of bone disorganization increased progressively from OI type 1 to types 3, 6, 8, and 14. In addition osteocyte lacunar area (Ot.Lc.Ar) was increased specifically in OI subtypes 1, 6, 8, and 14 bones, while osteocyte lacunar appearance was heterogeneous in size, shape, alignment, and spatial distribution in OI types 3, 6, 8, and 14, underscoring the case-specific alterations. Consistently, polarized light microscopy demonstrated increased green birefringence under polarized light microscopy in OI types 1 and 14 and reduced lamellar thickness in OI types 1, 6, and 8. At the molecular level, Raman spectroscopic analyses showed reduced mineral and organic matrix signals in OI bone, specifically OI type 3, indicating compromised mineralization and altered bone matrix composition. Together, these findings illustrate the potential that OI bone phenotype illustrates potential subtype-specific trends in bone microarchitecture, collagen disorganization, impaired lamellar bone formation, and deficits in bone mineral and matrix composition. This integrative analysis links genetic defects in collagen-related and non-collagen genes to multiscale alterations in bone tissue, providing mechanistic insight into OI pathophysiology and highlighting potential structural targets for individualized therapeutic strategies.
U2 - 10.1093/jbmrpl/ziag111
DO - 10.1093/jbmrpl/ziag111
M3 - Article
C2 - 42524503
SN - 2473-4039
VL - 10
JO - JBMR Plus
JF - JBMR Plus
IS - 8
M1 - ziag111
ER -