Highly Efficient ENU Mutagenesis in Zebrafish.

Ewart de Bruijn; Edwin Cuppen; Harma Feitsma

doi:10.1007/978-1-60327-977-2_1

Highly Efficient ENU Mutagenesis in Zebrafish.

Ewart de Bruijn^*
, Edwin Cuppen
, Harma Feitsma

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

20 Citations (Scopus)

Abstract

ENU (N-ethyl-N-nitrosourea) mutagenesis is a widely accepted and proven method to introduce random point mutations in the genome. Because there are no targeted knockout strategies available for zebrafish so far, random mutagenesis is currently the preferred method in both forward and reverse genetic approaches. To obtain high-density mutagenized zebrafish, six consecutive ENU treatments are applied at weekly intervals to adult male zebrafish by bathing them in ENU solution. With this procedure an average germ line mutation load of one mutation every 1.0 x 10(5)-1.5 x 10(5) basepairs is reached routinely in our lab.

Original language	English
Pages (from-to)	3-12
Number of pages	10
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	546
DOIs	https://doi.org/10.1007/978-1-60327-977-2_1
Publication status	Published - 2 Jul 2009

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title = "Highly Efficient ENU Mutagenesis in Zebrafish.",

abstract = "ENU (N-ethyl-N-nitrosourea) mutagenesis is a widely accepted and proven method to introduce random point mutations in the genome. Because there are no targeted knockout strategies available for zebrafish so far, random mutagenesis is currently the preferred method in both forward and reverse genetic approaches. To obtain high-density mutagenized zebrafish, six consecutive ENU treatments are applied at weekly intervals to adult male zebrafish by bathing them in ENU solution. With this procedure an average germ line mutation load of one mutation every 1.0 x 10(5)-1.5 x 10(5) basepairs is reached routinely in our lab.",

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Highly Efficient ENU Mutagenesis in Zebrafish.

Abstract

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