Heterologous biosynthesis and processing of preprovasopressin in Neuro2A neuroblastoma cells

F. M. de Bree; J. P.H. Burbach

doi:10.1016/0300-9084(94)90165-1

Heterologous biosynthesis and processing of preprovasopressin in Neuro2A neuroblastoma cells

F. M. de Bree
, J. P.H. Burbach^*

^*Corresponding author for this work

Translational Neuroscience

Research output: Contribution to journal › Article › Academic › peer-review

17 Citations (Scopus)

Abstract

To obtain a model for the sorting and processing of preprovasopressin (preproVP), rat VP cDNA was transfected in murine Neuro2A neuroblastoma cells, which do not express VP. The precursor of VP was expressed and processed into the authentic VP gene products VP, neurophysin (NP) and glycopeptide (GP) as determined with reversed phase HPLC and radioimmunoassay. In addition, Neuro2A-specific forms of NP and GP were observed, which may be produced in the constitutive secretory pathway in these cells.

Original language	English
Pages (from-to)	315-319
Number of pages	5
Journal	Biochimie
Volume	76
Issue number	3-4
DOIs	https://doi.org/10.1016/0300-9084(94)90165-1
Publication status	Published - 1994

Keywords

biosynthesis
Neuro2A
neuroblastoma
processing
vasopressin

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10.1016/0300-9084(94)90165-1

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title = "Heterologous biosynthesis and processing of preprovasopressin in Neuro2A neuroblastoma cells",

abstract = "To obtain a model for the sorting and processing of preprovasopressin (preproVP), rat VP cDNA was transfected in murine Neuro2A neuroblastoma cells, which do not express VP. The precursor of VP was expressed and processed into the authentic VP gene products VP, neurophysin (NP) and glycopeptide (GP) as determined with reversed phase HPLC and radioimmunoassay. In addition, Neuro2A-specific forms of NP and GP were observed, which may be produced in the constitutive secretory pathway in these cells.",

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year = "1994",

doi = "10.1016/0300-9084(94)90165-1",

language = "English",

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T1 - Heterologous biosynthesis and processing of preprovasopressin in Neuro2A neuroblastoma cells

AU - de Bree, F. M.

AU - Burbach, J. P.H.

PY - 1994

Y1 - 1994

N2 - To obtain a model for the sorting and processing of preprovasopressin (preproVP), rat VP cDNA was transfected in murine Neuro2A neuroblastoma cells, which do not express VP. The precursor of VP was expressed and processed into the authentic VP gene products VP, neurophysin (NP) and glycopeptide (GP) as determined with reversed phase HPLC and radioimmunoassay. In addition, Neuro2A-specific forms of NP and GP were observed, which may be produced in the constitutive secretory pathway in these cells.

AB - To obtain a model for the sorting and processing of preprovasopressin (preproVP), rat VP cDNA was transfected in murine Neuro2A neuroblastoma cells, which do not express VP. The precursor of VP was expressed and processed into the authentic VP gene products VP, neurophysin (NP) and glycopeptide (GP) as determined with reversed phase HPLC and radioimmunoassay. In addition, Neuro2A-specific forms of NP and GP were observed, which may be produced in the constitutive secretory pathway in these cells.

KW - biosynthesis

KW - Neuro2A

KW - neuroblastoma

KW - processing

KW - vasopressin

UR - https://www.scopus.com/pages/publications/0028022103

U2 - 10.1016/0300-9084(94)90165-1

DO - 10.1016/0300-9084(94)90165-1

M3 - Article

C2 - 7819342

AN - SCOPUS:0028022103

SN - 0300-9084

VL - 76

SP - 315

EP - 319

JO - Biochimie

JF - Biochimie

IS - 3-4

ER -

Heterologous biosynthesis and processing of preprovasopressin in Neuro2A neuroblastoma cells

Abstract

Keywords

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