Haemophilus influenzae is frequently detected with monoclonal antibody 8BD9 in sputum samples from patients with cystic fibrosis

L V Möller; G J Ruijs; H G Heijerman; J Dankert; L van Alphen

doi:10.1128/JCM.30.9.2495-2497.1992

Haemophilus influenzae is frequently detected with monoclonal antibody 8BD9 in sputum samples from patients with cystic fibrosis

L V Möller, G J Ruijs, H G Heijerman, J Dankert, L van Alphen

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

To determine the frequency of Haemophilus influenzae in sputum from patients with cystic fibrosis (CF), 477 sputum samples obtained from 86 CF patients were analyzed by standard culture and by the in situ immunoperoxidase staining technique with monoclonal antibody 8BD9. H. influenzae was isolated from 109 sputum samples (23%) from 45 patients (52%) and detected by immunoperoxidase staining in 175 sputum samples (37%) obtained from 63 patients (73%). The results of this study demonstrate the frequent presence of H. influenzae in sputum samples from CF patients.

Original language	English
Pages (from-to)	2495-7
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	30
Issue number	9
DOIs	https://doi.org/10.1128/JCM.30.9.2495-2497.1992
Publication status	Published - Sept 1992
Externally published	Yes

Keywords

Antibodies, Monoclonal
Bacteriological Techniques
Cystic Fibrosis/complications
Female
Haemophilus Infections/complications
Haemophilus influenzae/growth & development
Humans
Immunoenzyme Techniques
Male
Sputum/microbiology

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10.1128/JCM.30.9.2495-2497.1992

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@article{d7e5ab32416547b58b8e015d657d0bc2,

title = "Haemophilus influenzae is frequently detected with monoclonal antibody 8BD9 in sputum samples from patients with cystic fibrosis",

abstract = "To determine the frequency of Haemophilus influenzae in sputum from patients with cystic fibrosis (CF), 477 sputum samples obtained from 86 CF patients were analyzed by standard culture and by the in situ immunoperoxidase staining technique with monoclonal antibody 8BD9. H. influenzae was isolated from 109 sputum samples (23%) from 45 patients (52%) and detected by immunoperoxidase staining in 175 sputum samples (37%) obtained from 63 patients (73%). The results of this study demonstrate the frequent presence of H. influenzae in sputum samples from CF patients.",

keywords = "Antibodies, Monoclonal, Bacteriological Techniques, Cystic Fibrosis/complications, Female, Haemophilus Infections/complications, Haemophilus influenzae/growth & development, Humans, Immunoenzyme Techniques, Male, Sputum/microbiology",

author = "M{\"o}ller, {L V} and Ruijs, {G J} and Heijerman, {H G} and J Dankert and {van Alphen}, L",

year = "1992",

month = sep,

doi = "10.1128/JCM.30.9.2495-2497.1992",

language = "English",

volume = "30",

pages = "2495--7",

number = "9",

}

TY - JOUR

T1 - Haemophilus influenzae is frequently detected with monoclonal antibody 8BD9 in sputum samples from patients with cystic fibrosis

AU - Möller, L V

AU - Ruijs, G J

AU - Heijerman, H G

AU - Dankert, J

AU - van Alphen, L

PY - 1992/9

Y1 - 1992/9

N2 - To determine the frequency of Haemophilus influenzae in sputum from patients with cystic fibrosis (CF), 477 sputum samples obtained from 86 CF patients were analyzed by standard culture and by the in situ immunoperoxidase staining technique with monoclonal antibody 8BD9. H. influenzae was isolated from 109 sputum samples (23%) from 45 patients (52%) and detected by immunoperoxidase staining in 175 sputum samples (37%) obtained from 63 patients (73%). The results of this study demonstrate the frequent presence of H. influenzae in sputum samples from CF patients.

AB - To determine the frequency of Haemophilus influenzae in sputum from patients with cystic fibrosis (CF), 477 sputum samples obtained from 86 CF patients were analyzed by standard culture and by the in situ immunoperoxidase staining technique with monoclonal antibody 8BD9. H. influenzae was isolated from 109 sputum samples (23%) from 45 patients (52%) and detected by immunoperoxidase staining in 175 sputum samples (37%) obtained from 63 patients (73%). The results of this study demonstrate the frequent presence of H. influenzae in sputum samples from CF patients.

KW - Antibodies, Monoclonal

KW - Bacteriological Techniques

KW - Cystic Fibrosis/complications

KW - Female

KW - Haemophilus Infections/complications

KW - Haemophilus influenzae/growth & development

KW - Humans

KW - Immunoenzyme Techniques

KW - Male

KW - Sputum/microbiology

U2 - 10.1128/JCM.30.9.2495-2497.1992

DO - 10.1128/JCM.30.9.2495-2497.1992

M3 - Article

C2 - 1401024

SN - 0095-1137

VL - 30

SP - 2495

EP - 2497

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 9

ER -

Haemophilus influenzae is frequently detected with monoclonal antibody 8BD9 in sputum samples from patients with cystic fibrosis

Abstract

Keywords

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