GlioM&M: Web-based tool for studying circulating and infiltrating monocytes and macrophages in glioma

Erik R. Abels; Sybren L.N. Maas; Eric Tai; David T. Ting; Marike L.D. Broekman; Xandra O. Breakefield; Joseph El Khoury

doi:10.1038/s41598-020-66728-w

GlioM&M: Web-based tool for studying circulating and infiltrating monocytes and macrophages in glioma

Erik R. Abels
, Sybren L.N. Maas
, Eric Tai
, David T. Ting
, Marike L.D. Broekman
, Xandra O. Breakefield
, Joseph El Khoury^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Monocytes, macrophages and microglia make up a large part of the glioma environment and have an important role in maintaining and propagating glioma progression. Targeting these cells to inhibit their tumor-promoting effect and reprogramming them into an anti-tumor phenotype is a potential therapeutic approach for glioma. In this study we analyzed the transcriptomes of eight different monocyte subgroups derived from the brain and the blood of glioma-bearing mice. We compared the expression profile of blood-derived monocytes versus tumor-infiltrating monocytes and found increased expression of both pro- and anti-inflammatory pathways in tumor infiltrating monocytes. To help disseminate these datasets, we created a user-friendly web-based tool accessible at www.glioma-monocytes.com. This tool can be used for validation purposes and to elucidate gene expression profiles of tumor-interacting monocytes and macrophages as well as blood-derived circulating monocytes. This tool can also be used to identify new markers and targets for therapy in these different cell populations.

Original language	English
Article number	9898
Pages (from-to)	1-11
Journal	Scientific Reports
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41598-020-66728-w
Publication status	Published - 18 Jun 2020

Keywords

Animals
Biomarkers/metabolism
Brain Neoplasms/immunology
Cell Line, Tumor
Cytokines/metabolism
Databases, Factual
Disease Models, Animal
Glioma/immunology
Lymphocytes, Tumor-Infiltrating/cytology
Macrophages/cytology
Mice
Mice, Inbred C57BL
Monocytes/cytology
Transplantation, Homologous
Up-Regulation
User-Computer Interface

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@article{c827d90669194328879d55357103610e,

title = "GlioM\&M: Web-based tool for studying circulating and infiltrating monocytes and macrophages in glioma",

abstract = "Monocytes, macrophages and microglia make up a large part of the glioma environment and have an important role in maintaining and propagating glioma progression. Targeting these cells to inhibit their tumor-promoting effect and reprogramming them into an anti-tumor phenotype is a potential therapeutic approach for glioma. In this study we analyzed the transcriptomes of eight different monocyte subgroups derived from the brain and the blood of glioma-bearing mice. We compared the expression profile of blood-derived monocytes versus tumor-infiltrating monocytes and found increased expression of both pro- and anti-inflammatory pathways in tumor infiltrating monocytes. To help disseminate these datasets, we created a user-friendly web-based tool accessible at www.glioma-monocytes.com. This tool can be used for validation purposes and to elucidate gene expression profiles of tumor-interacting monocytes and macrophages as well as blood-derived circulating monocytes. This tool can also be used to identify new markers and targets for therapy in these different cell populations.",

keywords = "Animals, Biomarkers/metabolism, Brain Neoplasms/immunology, Cell Line, Tumor, Cytokines/metabolism, Databases, Factual, Disease Models, Animal, Glioma/immunology, Lymphocytes, Tumor-Infiltrating/cytology, Macrophages/cytology, Mice, Mice, Inbred C57BL, Monocytes/cytology, Transplantation, Homologous, Up-Regulation, User-Computer Interface",

author = "Abels, \{Erik R.\} and Maas, \{Sybren L.N.\} and Eric Tai and Ting, \{David T.\} and Broekman, \{Marike L.D.\} and Breakefield, \{Xandra O.\} and \{El Khoury\}, Joseph",

note = "Funding Information: We thank the Massachusetts General Hospital, Department of Pathology Flow and Image Cytometry Research Core. All members of the Breakefield, Tannous, Maguire, Bragg and El Khoury labs that suggested ideas during lab meetings are very much appreciated. We thank Ms. Suzanne McDavitt for skilled editorial assistance. Sybren Maas acknowledges support from the Dutch Nijbakker-Morra travel stipend and the Dutch Cancer Society (KWF) travel grant. Xandra Breakefield acknowledges NIH NCI CA179563, CA069246 and CA232103 grants for funding used to perform this research. U19 CA179563 is supported by the NIH Common Fund, through the Office of Strategic Coordination/Office of the NIH Director. Joseph El Khoury is funded by NIH grants 1RF1 AG051506 and R01 AI119065. Generation of vectors used in this study was supported by the NIH NS045776 grant. The MGH Department of Pathology Flow and Image Cytometry Research Core obtained support from the NIH Shared Instrumentation program with grants 1S10OD012027-01A1, 1S10OD016372-01, 1S10RR020936-01, and 1S10RR023440-01A1. Publisher Copyright: {\textcopyright} 2020, The Author(s). Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2020",

month = jun,

day = "18",

doi = "10.1038/s41598-020-66728-w",

language = "English",

volume = "10",

pages = "1--11",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Research",

number = "1",

}

TY - JOUR

T1 - GlioM&M

T2 - Web-based tool for studying circulating and infiltrating monocytes and macrophages in glioma

AU - Abels, Erik R.

AU - Maas, Sybren L.N.

AU - Tai, Eric

AU - Ting, David T.

AU - Broekman, Marike L.D.

AU - Breakefield, Xandra O.

AU - El Khoury, Joseph

N1 - Funding Information: We thank the Massachusetts General Hospital, Department of Pathology Flow and Image Cytometry Research Core. All members of the Breakefield, Tannous, Maguire, Bragg and El Khoury labs that suggested ideas during lab meetings are very much appreciated. We thank Ms. Suzanne McDavitt for skilled editorial assistance. Sybren Maas acknowledges support from the Dutch Nijbakker-Morra travel stipend and the Dutch Cancer Society (KWF) travel grant. Xandra Breakefield acknowledges NIH NCI CA179563, CA069246 and CA232103 grants for funding used to perform this research. U19 CA179563 is supported by the NIH Common Fund, through the Office of Strategic Coordination/Office of the NIH Director. Joseph El Khoury is funded by NIH grants 1RF1 AG051506 and R01 AI119065. Generation of vectors used in this study was supported by the NIH NS045776 grant. The MGH Department of Pathology Flow and Image Cytometry Research Core obtained support from the NIH Shared Instrumentation program with grants 1S10OD012027-01A1, 1S10OD016372-01, 1S10RR020936-01, and 1S10RR023440-01A1. Publisher Copyright: © 2020, The Author(s). Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/6/18

Y1 - 2020/6/18

N2 - Monocytes, macrophages and microglia make up a large part of the glioma environment and have an important role in maintaining and propagating glioma progression. Targeting these cells to inhibit their tumor-promoting effect and reprogramming them into an anti-tumor phenotype is a potential therapeutic approach for glioma. In this study we analyzed the transcriptomes of eight different monocyte subgroups derived from the brain and the blood of glioma-bearing mice. We compared the expression profile of blood-derived monocytes versus tumor-infiltrating monocytes and found increased expression of both pro- and anti-inflammatory pathways in tumor infiltrating monocytes. To help disseminate these datasets, we created a user-friendly web-based tool accessible at www.glioma-monocytes.com. This tool can be used for validation purposes and to elucidate gene expression profiles of tumor-interacting monocytes and macrophages as well as blood-derived circulating monocytes. This tool can also be used to identify new markers and targets for therapy in these different cell populations.

AB - Monocytes, macrophages and microglia make up a large part of the glioma environment and have an important role in maintaining and propagating glioma progression. Targeting these cells to inhibit their tumor-promoting effect and reprogramming them into an anti-tumor phenotype is a potential therapeutic approach for glioma. In this study we analyzed the transcriptomes of eight different monocyte subgroups derived from the brain and the blood of glioma-bearing mice. We compared the expression profile of blood-derived monocytes versus tumor-infiltrating monocytes and found increased expression of both pro- and anti-inflammatory pathways in tumor infiltrating monocytes. To help disseminate these datasets, we created a user-friendly web-based tool accessible at www.glioma-monocytes.com. This tool can be used for validation purposes and to elucidate gene expression profiles of tumor-interacting monocytes and macrophages as well as blood-derived circulating monocytes. This tool can also be used to identify new markers and targets for therapy in these different cell populations.

KW - Animals

KW - Biomarkers/metabolism

KW - Brain Neoplasms/immunology

KW - Cell Line, Tumor

KW - Cytokines/metabolism

KW - Databases, Factual

KW - Disease Models, Animal

KW - Glioma/immunology

KW - Lymphocytes, Tumor-Infiltrating/cytology

KW - Macrophages/cytology

KW - Mice

KW - Mice, Inbred C57BL

KW - Monocytes/cytology

KW - Transplantation, Homologous

KW - Up-Regulation

KW - User-Computer Interface

UR - https://www.scopus.com/pages/publications/85086711919

U2 - 10.1038/s41598-020-66728-w

DO - 10.1038/s41598-020-66728-w

M3 - Article

C2 - 32555306

AN - SCOPUS:85086711919

SN - 2045-2322

VL - 10

SP - 1

EP - 11

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 9898

ER -

GlioM&M: Web-based tool for studying circulating and infiltrating monocytes and macrophages in glioma

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Keywords

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