Genomic variation in two gametocyte non-producing Plasmodium falciparum clonal lines

Susana Campino; Ernest Diez Benavente; Samuel Assefa; Eloise Thompson; Laura G Drought; Catherine J Taylor; Zaria Gorvett; Celine K Carret; Christian Flueck; Al C Ivens; Dominic P Kwiatkowski; Pietro Alano; David A Baker; Taane G Clark

doi:10.1186/s12936-016-1254-1

Genomic variation in two gametocyte non-producing Plasmodium falciparum clonal lines

Susana Campino
, Ernest Diez Benavente
, Samuel Assefa
, Eloise Thompson
, Laura G Drought
, Catherine J Taylor
, Zaria Gorvett
, Celine K Carret
, Christian Flueck
, Al C Ivens
, Dominic P Kwiatkowski
, Pietro Alano
, David A Baker
, Taane G Clark

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: Transmission of the malaria parasite Plasmodium falciparum from humans to the mosquito vector requires differentiation of a sub-population of asexual forms replicating within red blood cells into non-dividing male and female gametocytes. The nature of the molecular mechanism underlying this key differentiation event required for malaria transmission is not fully understood.

METHODS: Whole genome sequencing was used to examine the genomic diversity of the gametocyte non-producing 3D7-derived lines F12 and A4. These lines were used in the recent detection of the PF3D7_1222600 locus (encoding PfAP2-G), which acts as a genetic master switch that triggers gametocyte development.

RESULTS: The evolutionary changes from the 3D7 parental strain through its derivatives F12 (culture-passage derived cloned line) and A4 (transgenic cloned line) were identified. The genetic differences including the formation of chimeric var genes are presented.

CONCLUSION: A genomics resource is provided for the further study of gametocytogenesis or other phenotypes using these parasite lines.

Original language	English
Pages (from-to)	229
Journal	Malaria Journal
Volume	15
DOIs	https://doi.org/10.1186/s12936-016-1254-1
Publication status	Published - 21 Apr 2016
Externally published	Yes

Keywords

Gametogenesis
Genome, Protozoan
Plasmodium falciparum/genetics
Polymorphism, Genetic
Sequence Analysis, DNA

Access to Document

10.1186/s12936-016-1254-1

Cite this

@article{03dc629022294e32b13710950c45d1e6,

title = "Genomic variation in two gametocyte non-producing Plasmodium falciparum clonal lines",

abstract = "BACKGROUND: Transmission of the malaria parasite Plasmodium falciparum from humans to the mosquito vector requires differentiation of a sub-population of asexual forms replicating within red blood cells into non-dividing male and female gametocytes. The nature of the molecular mechanism underlying this key differentiation event required for malaria transmission is not fully understood.METHODS: Whole genome sequencing was used to examine the genomic diversity of the gametocyte non-producing 3D7-derived lines F12 and A4. These lines were used in the recent detection of the PF3D7\_1222600 locus (encoding PfAP2-G), which acts as a genetic master switch that triggers gametocyte development.RESULTS: The evolutionary changes from the 3D7 parental strain through its derivatives F12 (culture-passage derived cloned line) and A4 (transgenic cloned line) were identified. The genetic differences including the formation of chimeric var genes are presented.CONCLUSION: A genomics resource is provided for the further study of gametocytogenesis or other phenotypes using these parasite lines.",

keywords = "Gametogenesis, Genome, Protozoan, Plasmodium falciparum/genetics, Polymorphism, Genetic, Sequence Analysis, DNA",

author = "Susana Campino and Benavente, \{Ernest Diez\} and Samuel Assefa and Eloise Thompson and Drought, \{Laura G\} and Taylor, \{Catherine J\} and Zaria Gorvett and Carret, \{Celine K\} and Christian Flueck and Ivens, \{Al C\} and Kwiatkowski, \{Dominic P\} and Pietro Alano and Baker, \{David A\} and Clark, \{Taane G\}",

year = "2016",

month = apr,

day = "21",

doi = "10.1186/s12936-016-1254-1",

language = "English",

volume = "15",

pages = "229",

journal = "Malaria Journal",

issn = "1475-2875",

publisher = "BioMed Central Ltd.",

}

TY - JOUR

T1 - Genomic variation in two gametocyte non-producing Plasmodium falciparum clonal lines

AU - Campino, Susana

AU - Benavente, Ernest Diez

AU - Assefa, Samuel

AU - Thompson, Eloise

AU - Drought, Laura G

AU - Taylor, Catherine J

AU - Gorvett, Zaria

AU - Carret, Celine K

AU - Flueck, Christian

AU - Ivens, Al C

AU - Kwiatkowski, Dominic P

AU - Alano, Pietro

AU - Baker, David A

AU - Clark, Taane G

PY - 2016/4/21

Y1 - 2016/4/21

N2 - BACKGROUND: Transmission of the malaria parasite Plasmodium falciparum from humans to the mosquito vector requires differentiation of a sub-population of asexual forms replicating within red blood cells into non-dividing male and female gametocytes. The nature of the molecular mechanism underlying this key differentiation event required for malaria transmission is not fully understood.METHODS: Whole genome sequencing was used to examine the genomic diversity of the gametocyte non-producing 3D7-derived lines F12 and A4. These lines were used in the recent detection of the PF3D7_1222600 locus (encoding PfAP2-G), which acts as a genetic master switch that triggers gametocyte development.RESULTS: The evolutionary changes from the 3D7 parental strain through its derivatives F12 (culture-passage derived cloned line) and A4 (transgenic cloned line) were identified. The genetic differences including the formation of chimeric var genes are presented.CONCLUSION: A genomics resource is provided for the further study of gametocytogenesis or other phenotypes using these parasite lines.

AB - BACKGROUND: Transmission of the malaria parasite Plasmodium falciparum from humans to the mosquito vector requires differentiation of a sub-population of asexual forms replicating within red blood cells into non-dividing male and female gametocytes. The nature of the molecular mechanism underlying this key differentiation event required for malaria transmission is not fully understood.METHODS: Whole genome sequencing was used to examine the genomic diversity of the gametocyte non-producing 3D7-derived lines F12 and A4. These lines were used in the recent detection of the PF3D7_1222600 locus (encoding PfAP2-G), which acts as a genetic master switch that triggers gametocyte development.RESULTS: The evolutionary changes from the 3D7 parental strain through its derivatives F12 (culture-passage derived cloned line) and A4 (transgenic cloned line) were identified. The genetic differences including the formation of chimeric var genes are presented.CONCLUSION: A genomics resource is provided for the further study of gametocytogenesis or other phenotypes using these parasite lines.

KW - Gametogenesis

KW - Genome, Protozoan

KW - Plasmodium falciparum/genetics

KW - Polymorphism, Genetic

KW - Sequence Analysis, DNA

U2 - 10.1186/s12936-016-1254-1

DO - 10.1186/s12936-016-1254-1

M3 - Article

C2 - 27098483

SN - 1475-2875

VL - 15

SP - 229

JO - Malaria Journal

JF - Malaria Journal

ER -

Genomic variation in two gametocyte non-producing Plasmodium falciparum clonal lines

Abstract

Keywords

Access to Document

Fingerprint

Cite this