Generation of antibody responses to pneumococcal capsular polysaccharides is independent of CD1 expression in mice

Leen Moens; Axel Jeurissen; Stefan Nierkens; Louis Boon; Luc Van Kaer; Ahmad Kasran; Greet Wuyts; Jan L Ceuppens; Xavier Bossuyt

doi:10.1128/IAI.01091-08

Generation of antibody responses to pneumococcal capsular polysaccharides is independent of CD1 expression in mice

Leen Moens, Axel Jeurissen, Stefan Nierkens, Louis Boon, Luc Van Kaer, Ahmad Kasran, Greet Wuyts, Jan L Ceuppens, Xavier Bossuyt

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Streptococcus pneumoniae is a bacterial microorganism that frequently causes serious infection, particularly in children and the elderly. Protection against infection with S. pneumoniae is based mainly on the generation of antibodies to the pneumococcal capsular polysaccharides (caps-PS), but the mechanisms responsible for the generation of anticapsular antibodies remain incompletely understood. The aim of the present study was to evaluate the role of CD1-restricted T cells in the antibody response to caps-PS. When immunized with Pneumo23, wild-type mice and CD1 knockout mice on BALB/c and C57BL/6 backgrounds generated immunoglobulin M (IgM) and IgG antibody responses to soluble caps-PS that were comparable. Similar results were obtained after immunization with heat-inactivated S. pneumoniae. The IgM and IgG antibody response of wild-type mice to Pneumo23 was not affected by an antagonizing monoclonal anti-CD1 antibody treatment. In summary, our data provide evidence that the antibody response to caps-PS is generated independently of CD1 expression.

Original language	English
Pages (from-to)	1976-80
Number of pages	5
Journal	Infection and Immunity
Volume	77
Issue number	5
DOIs	https://doi.org/10.1128/IAI.01091-08
Publication status	Published - 2009

Keywords

Animals
Antibodies, Bacterial
Antigens, CD1
Humans
Immunoglobulin G
Immunoglobulin M
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
Pneumococcal Vaccines
Streptococcus pneumoniae

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title = "Generation of antibody responses to pneumococcal capsular polysaccharides is independent of CD1 expression in mice",

abstract = "Streptococcus pneumoniae is a bacterial microorganism that frequently causes serious infection, particularly in children and the elderly. Protection against infection with S. pneumoniae is based mainly on the generation of antibodies to the pneumococcal capsular polysaccharides (caps-PS), but the mechanisms responsible for the generation of anticapsular antibodies remain incompletely understood. The aim of the present study was to evaluate the role of CD1-restricted T cells in the antibody response to caps-PS. When immunized with Pneumo23, wild-type mice and CD1 knockout mice on BALB/c and C57BL/6 backgrounds generated immunoglobulin M (IgM) and IgG antibody responses to soluble caps-PS that were comparable. Similar results were obtained after immunization with heat-inactivated S. pneumoniae. The IgM and IgG antibody response of wild-type mice to Pneumo23 was not affected by an antagonizing monoclonal anti-CD1 antibody treatment. In summary, our data provide evidence that the antibody response to caps-PS is generated independently of CD1 expression.",

keywords = "Animals, Antibodies, Bacterial, Antigens, CD1, Humans, Immunoglobulin G, Immunoglobulin M, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Knockout, Pneumococcal Vaccines, Streptococcus pneumoniae",

author = "Leen Moens and Axel Jeurissen and Stefan Nierkens and Louis Boon and {Van Kaer}, Luc and Ahmad Kasran and Greet Wuyts and Ceuppens, {Jan L} and Xavier Bossuyt",

year = "2009",

doi = "10.1128/IAI.01091-08",

language = "English",

volume = "77",

pages = "1976--80",

journal = "Infection and Immunity",

issn = "0019-9567",

publisher = "American Society for Microbiology",

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TY - JOUR

T1 - Generation of antibody responses to pneumococcal capsular polysaccharides is independent of CD1 expression in mice

AU - Moens, Leen

AU - Jeurissen, Axel

AU - Nierkens, Stefan

AU - Boon, Louis

AU - Van Kaer, Luc

AU - Kasran, Ahmad

AU - Wuyts, Greet

AU - Ceuppens, Jan L

AU - Bossuyt, Xavier

PY - 2009

Y1 - 2009

N2 - Streptococcus pneumoniae is a bacterial microorganism that frequently causes serious infection, particularly in children and the elderly. Protection against infection with S. pneumoniae is based mainly on the generation of antibodies to the pneumococcal capsular polysaccharides (caps-PS), but the mechanisms responsible for the generation of anticapsular antibodies remain incompletely understood. The aim of the present study was to evaluate the role of CD1-restricted T cells in the antibody response to caps-PS. When immunized with Pneumo23, wild-type mice and CD1 knockout mice on BALB/c and C57BL/6 backgrounds generated immunoglobulin M (IgM) and IgG antibody responses to soluble caps-PS that were comparable. Similar results were obtained after immunization with heat-inactivated S. pneumoniae. The IgM and IgG antibody response of wild-type mice to Pneumo23 was not affected by an antagonizing monoclonal anti-CD1 antibody treatment. In summary, our data provide evidence that the antibody response to caps-PS is generated independently of CD1 expression.

AB - Streptococcus pneumoniae is a bacterial microorganism that frequently causes serious infection, particularly in children and the elderly. Protection against infection with S. pneumoniae is based mainly on the generation of antibodies to the pneumococcal capsular polysaccharides (caps-PS), but the mechanisms responsible for the generation of anticapsular antibodies remain incompletely understood. The aim of the present study was to evaluate the role of CD1-restricted T cells in the antibody response to caps-PS. When immunized with Pneumo23, wild-type mice and CD1 knockout mice on BALB/c and C57BL/6 backgrounds generated immunoglobulin M (IgM) and IgG antibody responses to soluble caps-PS that were comparable. Similar results were obtained after immunization with heat-inactivated S. pneumoniae. The IgM and IgG antibody response of wild-type mice to Pneumo23 was not affected by an antagonizing monoclonal anti-CD1 antibody treatment. In summary, our data provide evidence that the antibody response to caps-PS is generated independently of CD1 expression.

KW - Animals

KW - Antibodies, Bacterial

KW - Antigens, CD1

KW - Humans

KW - Immunoglobulin G

KW - Immunoglobulin M

KW - Mice

KW - Mice, Inbred BALB C

KW - Mice, Inbred C57BL

KW - Mice, Knockout

KW - Pneumococcal Vaccines

KW - Streptococcus pneumoniae

U2 - 10.1128/IAI.01091-08

DO - 10.1128/IAI.01091-08

M3 - Article

C2 - 19188354

SN - 0019-9567

VL - 77

SP - 1976

EP - 1980

JO - Infection and Immunity

JF - Infection and Immunity

IS - 5

ER -

Generation of antibody responses to pneumococcal capsular polysaccharides is independent of CD1 expression in mice

Abstract

Keywords

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