Generation and characterization of human iPSC lines SANi001-A and SANi002-A from mobilized peripheral blood derived megakaryoblasts

Marten Hansen; Eszter Varga; Tatjana Wüst; Clemens Mellink; Anne-Marie van der Kevie-Kersemaekers; Marieke von Lindern; Emile van den Akker

doi:10.1016/j.scr.2017.10.002

Generation and characterization of human iPSC lines SANi001-A and SANi002-A from mobilized peripheral blood derived megakaryoblasts

Marten Hansen
, Eszter Varga
, Tatjana Wüst
, Clemens Mellink
, Anne-Marie van der Kevie-Kersemaekers
, Marieke von Lindern
, Emile van den Akker

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Mobilized peripheral blood (MPB) CD34+ cells were differentiated to CD34+/CD41+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population which was reprogrammed with a hOKSM self-silencing polycistronic lentiviral vector. Resulting iPSC showed normal karyotype and expression of pluripotency associated markers and in vitro spontaneous differentiation towards the 3 germ layers confirmed pluripotency of iPSC lines. Besides normal iPSC applications, these lines can be used as a control line for other megakaryoid origin iPSC and could be applied for epigenetic based research.

Original language	English
Pages (from-to)	42-45
Number of pages	4
Journal	Stem Cell Research
Volume	25
DOIs	https://doi.org/10.1016/j.scr.2017.10.002
Publication status	Published - Dec 2017
Externally published	Yes

Keywords

Cell Differentiation/physiology
Cells, Cultured
Humans
Immunohistochemistry
Induced Pluripotent Stem Cells/cytology
Karyotyping
Megakaryocyte Progenitor Cells/cytology

Access to Document

10.1016/j.scr.2017.10.002Licence: CC BY-NC-ND

Cite this

@article{464f4263aa8a49fda21e7d9871f9c92f,

title = "Generation and characterization of human iPSC lines SANi001-A and SANi002-A from mobilized peripheral blood derived megakaryoblasts",

abstract = "Mobilized peripheral blood (MPB) CD34+ cells were differentiated to CD34+/CD41+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population which was reprogrammed with a hOKSM self-silencing polycistronic lentiviral vector. Resulting iPSC showed normal karyotype and expression of pluripotency associated markers and in vitro spontaneous differentiation towards the 3 germ layers confirmed pluripotency of iPSC lines. Besides normal iPSC applications, these lines can be used as a control line for other megakaryoid origin iPSC and could be applied for epigenetic based research.",

keywords = "Cell Differentiation/physiology, Cells, Cultured, Humans, Immunohistochemistry, Induced Pluripotent Stem Cells/cytology, Karyotyping, Megakaryocyte Progenitor Cells/cytology",

author = "Marten Hansen and Eszter Varga and Tatjana W{\"u}st and Clemens Mellink and \{van der Kevie-Kersemaekers\}, Anne-Marie and \{von Lindern\}, Marieke and \{van den Akker\}, Emile",

note = "Publisher Copyright: {\textcopyright} 2017",

year = "2017",

month = dec,

doi = "10.1016/j.scr.2017.10.002",

language = "English",

volume = "25",

pages = "42--45",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

}

TY - JOUR

T1 - Generation and characterization of human iPSC lines SANi001-A and SANi002-A from mobilized peripheral blood derived megakaryoblasts

AU - Hansen, Marten

AU - Varga, Eszter

AU - Wüst, Tatjana

AU - Mellink, Clemens

AU - van der Kevie-Kersemaekers, Anne-Marie

AU - von Lindern, Marieke

AU - van den Akker, Emile

PY - 2017/12

Y1 - 2017/12

N2 - Mobilized peripheral blood (MPB) CD34+ cells were differentiated to CD34+/CD41+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population which was reprogrammed with a hOKSM self-silencing polycistronic lentiviral vector. Resulting iPSC showed normal karyotype and expression of pluripotency associated markers and in vitro spontaneous differentiation towards the 3 germ layers confirmed pluripotency of iPSC lines. Besides normal iPSC applications, these lines can be used as a control line for other megakaryoid origin iPSC and could be applied for epigenetic based research.

AB - Mobilized peripheral blood (MPB) CD34+ cells were differentiated to CD34+/CD41+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population which was reprogrammed with a hOKSM self-silencing polycistronic lentiviral vector. Resulting iPSC showed normal karyotype and expression of pluripotency associated markers and in vitro spontaneous differentiation towards the 3 germ layers confirmed pluripotency of iPSC lines. Besides normal iPSC applications, these lines can be used as a control line for other megakaryoid origin iPSC and could be applied for epigenetic based research.

KW - Cell Differentiation/physiology

KW - Cells, Cultured

KW - Humans

KW - Immunohistochemistry

KW - Induced Pluripotent Stem Cells/cytology

KW - Karyotyping

KW - Megakaryocyte Progenitor Cells/cytology

UR - https://www.scopus.com/pages/publications/85031497742

U2 - 10.1016/j.scr.2017.10.002

DO - 10.1016/j.scr.2017.10.002

M3 - Article

C2 - 29055227

SN - 1873-5061

VL - 25

SP - 42

EP - 45

JO - Stem Cell Research

JF - Stem Cell Research

ER -

Generation and characterization of human iPSC lines SANi001-A and SANi002-A from mobilized peripheral blood derived megakaryoblasts

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this