Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts

Marten Hansen; Eszter Varga; Tatjana Wüst; Nelleke Brouwer; Hugues Beauchemin; Clemens Mellink; Anne-Marie van der Kevie-Kersemaekers; Tarik Möröy; Bert van der Reijden; Marieke von Lindern; Emile van den Akker

doi:10.1016/j.scr.2016.12.004

Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts

Marten Hansen, Eszter Varga, Tatjana Wüst, Nelleke Brouwer, Hugues Beauchemin, Clemens Mellink, Anne-Marie van der Kevie-Kersemaekers, Tarik Möröy, Bert van der Reijden, Marieke von Lindern, Emile van den Akker

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Mobilized peripheral blood (MPB) CD34+ cells were cultured to CD41+/CD34+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population that was reprogramed by a hOKSM self-silencing polycistronic vector using lentiviral delivery. The generated induced pluripotent stem cell (iPSC) lines were tested for silencing of the reprogramming construct by flow cytometry. Pluripotency of MML-6838-Cl2 iPSC line was confirmed by expression of associated markers and by in vivo spontaneous differentiation towards the 3 germ layers. The genomic integrity of iPSC line was shown by karyotyping. The MML-6838-Cl2 iPSC is, to our knowledge, the first to be generated from megakaryoblasts.

Original language	English
Pages (from-to)	26-28
Number of pages	3
Journal	Stem Cell Research
Volume	18
DOIs	https://doi.org/10.1016/j.scr.2016.12.004
Publication status	Published - Jan 2017
Externally published	Yes

Keywords

Animals
Antigens, CD34/metabolism
Cell Line
Cellular Reprogramming
Genetic Vectors/genetics
Hematopoietic Stem Cells/cytology
Humans
Induced Pluripotent Stem Cells/cytology
Karyotype
Lentivirus/genetics
Male
Megakaryocyte Progenitor Cells/cytology
Mice
Mice, Inbred NOD
Mice, SCID
Platelet Membrane Glycoprotein IIb/metabolism
Teratoma/metabolism
Transcription Factors/genetics

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Hansen, M., Varga, E., Wüst, T., Brouwer, N., Beauchemin, H., Mellink, C., van der Kevie-Kersemaekers, A.-M., Möröy, T., van der Reijden, B., von Lindern, M., & van den Akker, E. (2017). Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts. Stem Cell Research, 18, 26-28. https://doi.org/10.1016/j.scr.2016.12.004

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title = "Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts",

abstract = "Mobilized peripheral blood (MPB) CD34+ cells were cultured to CD41+/CD34+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population that was reprogramed by a hOKSM self-silencing polycistronic vector using lentiviral delivery. The generated induced pluripotent stem cell (iPSC) lines were tested for silencing of the reprogramming construct by flow cytometry. Pluripotency of MML-6838-Cl2 iPSC line was confirmed by expression of associated markers and by in vivo spontaneous differentiation towards the 3 germ layers. The genomic integrity of iPSC line was shown by karyotyping. The MML-6838-Cl2 iPSC is, to our knowledge, the first to be generated from megakaryoblasts.",

keywords = "Animals, Antigens, CD34/metabolism, Cell Line, Cellular Reprogramming, Genetic Vectors/genetics, Hematopoietic Stem Cells/cytology, Humans, Induced Pluripotent Stem Cells/cytology, Karyotype, Lentivirus/genetics, Male, Megakaryocyte Progenitor Cells/cytology, Mice, Mice, Inbred NOD, Mice, SCID, Platelet Membrane Glycoprotein IIb/metabolism, Teratoma/metabolism, Transcription Factors/genetics",

author = "Marten Hansen and Eszter Varga and Tatjana W{\"u}st and Nelleke Brouwer and Hugues Beauchemin and Clemens Mellink and {van der Kevie-Kersemaekers}, Anne-Marie and Tarik M{\"o}r{\"o}y and {van der Reijden}, Bert and {von Lindern}, Marieke and {van den Akker}, Emile",

note = "Publisher Copyright: {\textcopyright} 2016",

year = "2017",

month = jan,

doi = "10.1016/j.scr.2016.12.004",

language = "English",

volume = "18",

pages = "26--28",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

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Hansen, M, Varga, E, Wüst, T, Brouwer, N, Beauchemin, H, Mellink, C, van der Kevie-Kersemaekers, A-M, Möröy, T, van der Reijden, B, von Lindern, M & van den Akker, E 2017, 'Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts', Stem Cell Research, vol. 18, pp. 26-28. https://doi.org/10.1016/j.scr.2016.12.004

TY - JOUR

T1 - Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts

AU - Hansen, Marten

AU - Varga, Eszter

AU - Wüst, Tatjana

AU - Brouwer, Nelleke

AU - Beauchemin, Hugues

AU - Mellink, Clemens

AU - van der Kevie-Kersemaekers, Anne-Marie

AU - Möröy, Tarik

AU - van der Reijden, Bert

AU - von Lindern, Marieke

AU - van den Akker, Emile

PY - 2017/1

Y1 - 2017/1

N2 - Mobilized peripheral blood (MPB) CD34+ cells were cultured to CD41+/CD34+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population that was reprogramed by a hOKSM self-silencing polycistronic vector using lentiviral delivery. The generated induced pluripotent stem cell (iPSC) lines were tested for silencing of the reprogramming construct by flow cytometry. Pluripotency of MML-6838-Cl2 iPSC line was confirmed by expression of associated markers and by in vivo spontaneous differentiation towards the 3 germ layers. The genomic integrity of iPSC line was shown by karyotyping. The MML-6838-Cl2 iPSC is, to our knowledge, the first to be generated from megakaryoblasts.

AB - Mobilized peripheral blood (MPB) CD34+ cells were cultured to CD41+/CD34+ megakaryoblasts. Cells were sorted to obtain a pure megakaryoblast population that was reprogramed by a hOKSM self-silencing polycistronic vector using lentiviral delivery. The generated induced pluripotent stem cell (iPSC) lines were tested for silencing of the reprogramming construct by flow cytometry. Pluripotency of MML-6838-Cl2 iPSC line was confirmed by expression of associated markers and by in vivo spontaneous differentiation towards the 3 germ layers. The genomic integrity of iPSC line was shown by karyotyping. The MML-6838-Cl2 iPSC is, to our knowledge, the first to be generated from megakaryoblasts.

KW - Animals

KW - Antigens, CD34/metabolism

KW - Cell Line

KW - Cellular Reprogramming

KW - Genetic Vectors/genetics

KW - Hematopoietic Stem Cells/cytology

KW - Humans

KW - Induced Pluripotent Stem Cells/cytology

KW - Karyotype

KW - Lentivirus/genetics

KW - Male

KW - Megakaryocyte Progenitor Cells/cytology

KW - Mice

KW - Mice, Inbred NOD

KW - Mice, SCID

KW - Platelet Membrane Glycoprotein IIb/metabolism

KW - Teratoma/metabolism

KW - Transcription Factors/genetics

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U2 - 10.1016/j.scr.2016.12.004

DO - 10.1016/j.scr.2016.12.004

M3 - Article

C2 - 28395797

SN - 1873-5061

VL - 18

SP - 26

EP - 28

JO - Stem Cell Research

JF - Stem Cell Research

ER -

Generation and characterization of human iPSC line MML-6838-Cl2 from mobilized peripheral blood derived megakaryoblasts

Abstract

Keywords

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