Generation and analysis of mouse intestinal tumors and organoids harboring APC and K-Ras mutations

Johan H van Es, Hans Clevers

Research output: Contribution to journalArticleAcademicpeer-review


Genetically engineered mouse models of intestinal cancer are experimental systems in which mice are genetically manipulated to develop malignancies in the gastrointestinal tract. These models enable researchers to study the mechanisms of onset, progression, and metastasis of the disease. They also provide a valuable biological system which is suitable for testing (novel) drugs in vivo. Recently, an in vitro culture model has been established in which intestinal epithelial stem cells can grow into three-dimensional, ever-expanding epithelial organoids that retain their original organ identity and genetic stability. This culture system has been applied to diseased epithelia, such as adenoma, adenocarcinoma, and Barrett's epithelium. These organoids can be particularly useful for studying the mechanisms of intestinal tumors and to test (novel) drugs in vitro. Here, we describe our current laboratory protocols to generate and analyze intestinal tumors and organoids harboring APC and K-Ras double mutations.

Original languageEnglish
Pages (from-to)125-44
Number of pages20
JournalMethods in molecular biology
Publication statusPublished - 2015


  • Adenomatous Polyposis Coli Protein
  • Animals
  • Breeding
  • Cell Line, Tumor
  • Cryopreservation
  • DNA
  • Female
  • Genotyping Techniques
  • Humans
  • Immunohistochemistry
  • Integrases
  • Intestinal Neoplasms
  • Intestine, Small
  • Male
  • Mice
  • Mutation
  • Organoids
  • Paraffin Embedding
  • Polymerase Chain Reaction
  • Proto-Oncogene Proteins p21(ras)
  • Tamoxifen
  • Tissue Fixation


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