@inbook{aba3a330b35e44cd80cbceefee5051dc,
title = "Gene transfer in cardiomyocytes derived from ES and iPS cells",
abstract = "The advent of human induced pluripotent stem cell (hiPSC) technology has produced patient-specific hiPSC derived cardiomyocytes (hiPSC-CMs) that can be used as a platform to study cardiac diseases and to explore new therapies. The ability to genetically manipulate hiPSC-CMs not only is essential for identifying the structural and/or functional role of a protein but can also provide valuable information regarding therapeutic applications. In this chapter, we describe protocols for culture, maintenance, and cardiac differentiation of hiPSCs. Then, we provide a basic procedure to transduce hiPSC-CMs.",
keywords = "Cardiac differentiation, Gene therapy, HPSC-derived cardiomyocytes, Human pluripotent stem cells (hPSCs), Transduction",
author = "Francesca Stillitano and Ioannis Karakikes and Hajjar, {Roger J.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2017.",
year = "2017",
doi = "10.1007/978-1-4939-6588-5_12",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "183--193",
booktitle = "Methods in Molecular Biology",
}