Functional and phenotypic differences of monocyte-derived dendritic cells from allergic and nonallergic patients

M M van den Heuvel; D D Vanhee; P E Postmus; E C Hoefsmit; R H Beelen

doi:10.1016/S0091-6749(98)70198-8

Functional and phenotypic differences of monocyte-derived dendritic cells from allergic and nonallergic patients

M M van den Heuvel
, D D Vanhee
, P E Postmus
, E C Hoefsmit
, R H Beelen

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: Several studies suggest a role for dendritic cell in the pathogenesis of allergic disease.

OBJECTIVE: The purpose of this study was to compare function and phenotype of monocyte-derived dendritic cells (MoDC) from allergic asthmatic patients and healthy control subjects.

METHODS: MoDCs were developed by incubating adherent monocytes for 5 days with IL-4 and granulocyte-macrophage colony-stimulating factor. Phenotype was assessed with flow cytometry, and the antigen-presenting function was assessed with the allogeneic mixed leukocyte reaction and an autologous specific antigen presentation.

RESULTS: The morphology of the MoDCs was characteristic for immature dendritic cells. MoDCs from allergic asthmatic patients showed phenotypic differences in the expression of HLA-DR, CD11b, and the high-affinity receptor for IgE. A clearly enhanced accessory potential of MoDCs from atopic asthmatic patients in the mixed leukocyte reaction was also shown. Moreover, house dust mite-specific T-cell proliferation was increased.

CONCLUSION: This study suggests the involvement of dendritic cells in the pathogenesis of atopic asthma by an increased immunostimulatory capacity of MoDCs.

Original language	English
Pages (from-to)	90-5
Number of pages	6
Journal	The Journal of Allergy and Clinical Immunology
Volume	101
Issue number	1 Pt 1
DOIs	https://doi.org/10.1016/S0091-6749(98)70198-8
Publication status	Published - Jan 1998
Externally published	Yes

Keywords

Antigen Presentation
Antigens, CD/metabolism
Case-Control Studies
Cell Differentiation
Dendritic Cells/immunology
Humans
Hypersensitivity/etiology
In Vitro Techniques
Lymphocyte Activation
Lymphocyte Culture Test, Mixed
Macrophage-1 Antigen/metabolism
Monocytes/immunology
Phenotype
Receptors, IgE/metabolism
T-Lymphocytes/immunology

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title = "Functional and phenotypic differences of monocyte-derived dendritic cells from allergic and nonallergic patients",

abstract = "BACKGROUND: Several studies suggest a role for dendritic cell in the pathogenesis of allergic disease.OBJECTIVE: The purpose of this study was to compare function and phenotype of monocyte-derived dendritic cells (MoDC) from allergic asthmatic patients and healthy control subjects.METHODS: MoDCs were developed by incubating adherent monocytes for 5 days with IL-4 and granulocyte-macrophage colony-stimulating factor. Phenotype was assessed with flow cytometry, and the antigen-presenting function was assessed with the allogeneic mixed leukocyte reaction and an autologous specific antigen presentation.RESULTS: The morphology of the MoDCs was characteristic for immature dendritic cells. MoDCs from allergic asthmatic patients showed phenotypic differences in the expression of HLA-DR, CD11b, and the high-affinity receptor for IgE. A clearly enhanced accessory potential of MoDCs from atopic asthmatic patients in the mixed leukocyte reaction was also shown. Moreover, house dust mite-specific T-cell proliferation was increased.CONCLUSION: This study suggests the involvement of dendritic cells in the pathogenesis of atopic asthma by an increased immunostimulatory capacity of MoDCs.",

keywords = "Antigen Presentation, Antigens, CD/metabolism, Case-Control Studies, Cell Differentiation, Dendritic Cells/immunology, Humans, Hypersensitivity/etiology, In Vitro Techniques, Lymphocyte Activation, Lymphocyte Culture Test, Mixed, Macrophage-1 Antigen/metabolism, Monocytes/immunology, Phenotype, Receptors, IgE/metabolism, T-Lymphocytes/immunology",

author = "\{van den Heuvel\}, \{M M\} and Vanhee, \{D D\} and Postmus, \{P E\} and Hoefsmit, \{E C\} and Beelen, \{R H\}",

year = "1998",

month = jan,

doi = "10.1016/S0091-6749(98)70198-8",

language = "English",

volume = "101",

pages = "90--5",

journal = "The Journal of Allergy and Clinical Immunology",

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TY - JOUR

T1 - Functional and phenotypic differences of monocyte-derived dendritic cells from allergic and nonallergic patients

AU - van den Heuvel, M M

AU - Vanhee, D D

AU - Postmus, P E

AU - Hoefsmit, E C

AU - Beelen, R H

PY - 1998/1

Y1 - 1998/1

N2 - BACKGROUND: Several studies suggest a role for dendritic cell in the pathogenesis of allergic disease.OBJECTIVE: The purpose of this study was to compare function and phenotype of monocyte-derived dendritic cells (MoDC) from allergic asthmatic patients and healthy control subjects.METHODS: MoDCs were developed by incubating adherent monocytes for 5 days with IL-4 and granulocyte-macrophage colony-stimulating factor. Phenotype was assessed with flow cytometry, and the antigen-presenting function was assessed with the allogeneic mixed leukocyte reaction and an autologous specific antigen presentation.RESULTS: The morphology of the MoDCs was characteristic for immature dendritic cells. MoDCs from allergic asthmatic patients showed phenotypic differences in the expression of HLA-DR, CD11b, and the high-affinity receptor for IgE. A clearly enhanced accessory potential of MoDCs from atopic asthmatic patients in the mixed leukocyte reaction was also shown. Moreover, house dust mite-specific T-cell proliferation was increased.CONCLUSION: This study suggests the involvement of dendritic cells in the pathogenesis of atopic asthma by an increased immunostimulatory capacity of MoDCs.

AB - BACKGROUND: Several studies suggest a role for dendritic cell in the pathogenesis of allergic disease.OBJECTIVE: The purpose of this study was to compare function and phenotype of monocyte-derived dendritic cells (MoDC) from allergic asthmatic patients and healthy control subjects.METHODS: MoDCs were developed by incubating adherent monocytes for 5 days with IL-4 and granulocyte-macrophage colony-stimulating factor. Phenotype was assessed with flow cytometry, and the antigen-presenting function was assessed with the allogeneic mixed leukocyte reaction and an autologous specific antigen presentation.RESULTS: The morphology of the MoDCs was characteristic for immature dendritic cells. MoDCs from allergic asthmatic patients showed phenotypic differences in the expression of HLA-DR, CD11b, and the high-affinity receptor for IgE. A clearly enhanced accessory potential of MoDCs from atopic asthmatic patients in the mixed leukocyte reaction was also shown. Moreover, house dust mite-specific T-cell proliferation was increased.CONCLUSION: This study suggests the involvement of dendritic cells in the pathogenesis of atopic asthma by an increased immunostimulatory capacity of MoDCs.

KW - Antigen Presentation

KW - Antigens, CD/metabolism

KW - Case-Control Studies

KW - Cell Differentiation

KW - Dendritic Cells/immunology

KW - Humans

KW - Hypersensitivity/etiology

KW - In Vitro Techniques

KW - Lymphocyte Activation

KW - Lymphocyte Culture Test, Mixed

KW - Macrophage-1 Antigen/metabolism

KW - Monocytes/immunology

KW - Phenotype

KW - Receptors, IgE/metabolism

KW - T-Lymphocytes/immunology

U2 - 10.1016/S0091-6749(98)70198-8

DO - 10.1016/S0091-6749(98)70198-8

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C2 - 9449506

SN - 0091-6749

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EP - 95

JO - The Journal of Allergy and Clinical Immunology

JF - The Journal of Allergy and Clinical Immunology

IS - 1 Pt 1

ER -

Functional and phenotypic differences of monocyte-derived dendritic cells from allergic and nonallergic patients

Abstract

Keywords

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