Fluorescence diagnosis in keratinocytic intraepidermal neoplasias

Tim Smits; Marloes M Kleinpenning; Willeke A M Blokx; Peter C M van de Kerkhof; Piet E J van Erp; Marie-Jeanne P Gerritsen

doi:10.1016/j.jaad.2007.06.031

Fluorescence diagnosis in keratinocytic intraepidermal neoplasias

Tim Smits
, Marloes M Kleinpenning
, Willeke A M Blokx
, Peter C M van de Kerkhof
, Piet E J van Erp
, Marie-Jeanne P Gerritsen

Department of Pathology

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: As different tissue types have distinct capabilities to accumulate protoporphyrin-IX, fluorescence diagnosis with aminolevulinic acid-induced porphyrin (FDAP) could be used to discriminate between different tissue types.

OBJECTIVE: Protoporphyrin-IX accumulation and proliferation were studied in cutaneous squamous (pre)malignancies to see whether FDAP could be used to discriminate between different stages of keratinocytic intraepidermal neoplasia or proliferative status.

METHODS: FDAP was performed in 14 patients (86 lesions) and biopsy specimens were taken, on which (immuno)histochemistry was performed for histopathologic classification and assessment of Ki67-antigen expression. Stratum corneum thickness was also measured.

RESULTS: The fluorescence ratio (lesional:nonlesional skin) showed neither significant differences between the different keratinocytic intraepidermal neoplasia stages, nor between different levels of Ki67-antigen expression. Macroscopic fluorescence intensity and stratum corneum thickness were negatively correlated.

LIMITATIONS: Relatively few malignancies were biopsied.

CONCLUSIONS: With FDAP we were not able to discriminate between keratinocytic intraepidermal neoplasia lesions or proliferative activity. However, hyperkeratosis appeared to be an important determinant in variations in macroscopic fluorescence intensity.

Original language	English
Pages (from-to)	824-31
Number of pages	8
Journal	Journal of the American Academy of Dermatology
Volume	57
Issue number	5
DOIs	https://doi.org/10.1016/j.jaad.2007.06.031
Publication status	Published - Nov 2007

Keywords

Adult
Aged
Aged, 80 and over
Aminolevulinic Acid
Carcinoma, Squamous Cell/diagnosis
Cell Proliferation
Diagnosis, Computer-Assisted
Diagnosis, Differential
Epidermis/pathology
Female
Fluorescence
Humans
Immunohistochemistry
Keratinocytes/pathology
Ki-67 Antigen/analysis
Male
Middle Aged
Neoplasm Staging
Photosensitizing Agents
Protoporphyrins
Skin Neoplasms/diagnosis

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10.1016/j.jaad.2007.06.031

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title = "Fluorescence diagnosis in keratinocytic intraepidermal neoplasias",

abstract = "BACKGROUND: As different tissue types have distinct capabilities to accumulate protoporphyrin-IX, fluorescence diagnosis with aminolevulinic acid-induced porphyrin (FDAP) could be used to discriminate between different tissue types.OBJECTIVE: Protoporphyrin-IX accumulation and proliferation were studied in cutaneous squamous (pre)malignancies to see whether FDAP could be used to discriminate between different stages of keratinocytic intraepidermal neoplasia or proliferative status.METHODS: FDAP was performed in 14 patients (86 lesions) and biopsy specimens were taken, on which (immuno)histochemistry was performed for histopathologic classification and assessment of Ki67-antigen expression. Stratum corneum thickness was also measured.RESULTS: The fluorescence ratio (lesional:nonlesional skin) showed neither significant differences between the different keratinocytic intraepidermal neoplasia stages, nor between different levels of Ki67-antigen expression. Macroscopic fluorescence intensity and stratum corneum thickness were negatively correlated.LIMITATIONS: Relatively few malignancies were biopsied.CONCLUSIONS: With FDAP we were not able to discriminate between keratinocytic intraepidermal neoplasia lesions or proliferative activity. However, hyperkeratosis appeared to be an important determinant in variations in macroscopic fluorescence intensity.",

keywords = "Adult, Aged, Aged, 80 and over, Aminolevulinic Acid, Carcinoma, Squamous Cell/diagnosis, Cell Proliferation, Diagnosis, Computer-Assisted, Diagnosis, Differential, Epidermis/pathology, Female, Fluorescence, Humans, Immunohistochemistry, Keratinocytes/pathology, Ki-67 Antigen/analysis, Male, Middle Aged, Neoplasm Staging, Photosensitizing Agents, Protoporphyrins, Skin Neoplasms/diagnosis",

author = "Tim Smits and Kleinpenning, \{Marloes M\} and Blokx, \{Willeke A M\} and \{van de Kerkhof\}, \{Peter C M\} and \{van Erp\}, \{Piet E J\} and Gerritsen, \{Marie-Jeanne P\}",

year = "2007",

month = nov,

doi = "10.1016/j.jaad.2007.06.031",

language = "English",

volume = "57",

pages = "824--31",

journal = "Journal of the American Academy of Dermatology",

issn = "0190-9622",

publisher = "Elsevier",

number = "5",

}

TY - JOUR

T1 - Fluorescence diagnosis in keratinocytic intraepidermal neoplasias

AU - Smits, Tim

AU - Kleinpenning, Marloes M

AU - Blokx, Willeke A M

AU - van de Kerkhof, Peter C M

AU - van Erp, Piet E J

AU - Gerritsen, Marie-Jeanne P

PY - 2007/11

Y1 - 2007/11

N2 - BACKGROUND: As different tissue types have distinct capabilities to accumulate protoporphyrin-IX, fluorescence diagnosis with aminolevulinic acid-induced porphyrin (FDAP) could be used to discriminate between different tissue types.OBJECTIVE: Protoporphyrin-IX accumulation and proliferation were studied in cutaneous squamous (pre)malignancies to see whether FDAP could be used to discriminate between different stages of keratinocytic intraepidermal neoplasia or proliferative status.METHODS: FDAP was performed in 14 patients (86 lesions) and biopsy specimens were taken, on which (immuno)histochemistry was performed for histopathologic classification and assessment of Ki67-antigen expression. Stratum corneum thickness was also measured.RESULTS: The fluorescence ratio (lesional:nonlesional skin) showed neither significant differences between the different keratinocytic intraepidermal neoplasia stages, nor between different levels of Ki67-antigen expression. Macroscopic fluorescence intensity and stratum corneum thickness were negatively correlated.LIMITATIONS: Relatively few malignancies were biopsied.CONCLUSIONS: With FDAP we were not able to discriminate between keratinocytic intraepidermal neoplasia lesions or proliferative activity. However, hyperkeratosis appeared to be an important determinant in variations in macroscopic fluorescence intensity.

AB - BACKGROUND: As different tissue types have distinct capabilities to accumulate protoporphyrin-IX, fluorescence diagnosis with aminolevulinic acid-induced porphyrin (FDAP) could be used to discriminate between different tissue types.OBJECTIVE: Protoporphyrin-IX accumulation and proliferation were studied in cutaneous squamous (pre)malignancies to see whether FDAP could be used to discriminate between different stages of keratinocytic intraepidermal neoplasia or proliferative status.METHODS: FDAP was performed in 14 patients (86 lesions) and biopsy specimens were taken, on which (immuno)histochemistry was performed for histopathologic classification and assessment of Ki67-antigen expression. Stratum corneum thickness was also measured.RESULTS: The fluorescence ratio (lesional:nonlesional skin) showed neither significant differences between the different keratinocytic intraepidermal neoplasia stages, nor between different levels of Ki67-antigen expression. Macroscopic fluorescence intensity and stratum corneum thickness were negatively correlated.LIMITATIONS: Relatively few malignancies were biopsied.CONCLUSIONS: With FDAP we were not able to discriminate between keratinocytic intraepidermal neoplasia lesions or proliferative activity. However, hyperkeratosis appeared to be an important determinant in variations in macroscopic fluorescence intensity.

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KW - Aged

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KW - Diagnosis, Computer-Assisted

KW - Diagnosis, Differential

KW - Epidermis/pathology

KW - Female

KW - Fluorescence

KW - Humans

KW - Immunohistochemistry

KW - Keratinocytes/pathology

KW - Ki-67 Antigen/analysis

KW - Male

KW - Middle Aged

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KW - Photosensitizing Agents

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SN - 0190-9622

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SP - 824

EP - 831

JO - Journal of the American Academy of Dermatology

JF - Journal of the American Academy of Dermatology

IS - 5

ER -

Fluorescence diagnosis in keratinocytic intraepidermal neoplasias

Abstract

Keywords

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