Fluctuations in anti-Müllerian hormone levels throughout the menstrual cycle parallel fluctuations in the antral follicle count: A cohort study

Martine Depmann; Jeroen van Disseldorp; Simone L. Broer; Marinus J C Eijkemans; Joop S E Laven; Jenny A. Visser; Yolanda B. de Rijke; Ben W. Mol; Frank J M Broekmans

doi:10.1111/aogs.12886

Fluctuations in anti-Müllerian hormone levels throughout the menstrual cycle parallel fluctuations in the antral follicle count: A cohort study

Martine Depmann^*
, Jeroen van Disseldorp
, Simone L. Broer
, Marinus J C Eijkemans
, Joop S E Laven
, Jenny A. Visser
, Yolanda B. de Rijke
, Ben W. Mol
, Frank J M Broekmans

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Introduction

In this prospective cohort study we aimed to investigate the hypothesis that fluctuations in anti-Müllerian hormone levels stem from fluctuations in the number of antral follicles.

Material and methods

Repeated measurements of anti-Müllerian hormone and antral follicles (follicles 2–8 mm) were performed in 44 women with a regular cycle, during one menstrual cycle. If our hypothesis that anti-Müllerian hormone fluctuations stem from fluctuations in the antral follicles is correct, a fluctuation in the antral follicles would result in an equal and parallel shift in anti-Müllerian hormone. Hence, the difference between antral follicles and anti-Müllerian hormone would remain constant over time. A mixed model analysis, assessing the stability between anti-Müllerian hormone and antral follicles, was performed using the difference between logantral follicles and loganti-Müllerian hormone. Cohen's D was calculated for the largest of fixed effects in order to assess stability in relative distance between antral follicles and anti-Müllerian hormone. To assess if fluctuation in anti-Müllerian hormone or antral follicles originated from between-subject fluctuation, or from within subject fluctuation, the intra-class correlation coefficient was calculated.

Results

Mixed model analysis and Cohen's D (0.12) confirmed the stability of the difference between logantral follicles and loganti-Müllerian hormone and so confirmed our hypothesis. The good intra-class correlation coefficient (0.73) indicated a small contribution of within-subject variation to anti-Müllerian hormone fluctuations.

Conclusions

Fluctuations in anti-Müllerian hormone levels parallel fluctuations in antral follicles, suggesting that anti-Müllerian hormone levels are closely linked to variation in the antral follicles. This knowledge adds to the basic understanding of the origin of anti-Müllerian hormone and could aid in interpretation of individual anti-Müllerian hormone levels.

Original language	English
Pages (from-to)	820-828
Journal	Acta Obstetricia et Gynecologica Scandinavica
Volume	95
Issue number	7
DOIs	https://doi.org/10.1111/aogs.12886
Publication status	Published - Jul 2016

Keywords

Anti-Müllerian hormone
Antral follicle count
Fluctuation

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10.1111/aogs.12886

FluctuationsFinal published version, 596 KBLicence: Taverne

Cite this

Depmann, M., van Disseldorp, J., Broer, S. L., Eijkemans, M. J. C., Laven, J. S. E., Visser, J. A., de Rijke, Y. B., Mol, B. W., & Broekmans, F. J. M. (2016). Fluctuations in anti-Müllerian hormone levels throughout the menstrual cycle parallel fluctuations in the antral follicle count: A cohort study. Acta Obstetricia et Gynecologica Scandinavica, 95(7), 820-828. https://doi.org/10.1111/aogs.12886

@article{9b41f2bb3a744de38ee9433008282726,

title = "Fluctuations in anti-M{\"u}llerian hormone levels throughout the menstrual cycle parallel fluctuations in the antral follicle count: A cohort study",

abstract = "Introduction In this prospective cohort study we aimed to investigate the hypothesis that fluctuations in anti-M{\"u}llerian hormone levels stem from fluctuations in the number of antral follicles. Material and methods Repeated measurements of anti-M{\"u}llerian hormone and antral follicles (follicles 2–8 mm) were performed in 44 women with a regular cycle, during one menstrual cycle. If our hypothesis that anti-M{\"u}llerian hormone fluctuations stem from fluctuations in the antral follicles is correct, a fluctuation in the antral follicles would result in an equal and parallel shift in anti-M{\"u}llerian hormone. Hence, the difference between antral follicles and anti-M{\"u}llerian hormone would remain constant over time. A mixed model analysis, assessing the stability between anti-M{\"u}llerian hormone and antral follicles, was performed using the difference between logantral follicles and loganti-M{\"u}llerian hormone. Cohen's D was calculated for the largest of fixed effects in order to assess stability in relative distance between antral follicles and anti-M{\"u}llerian hormone. To assess if fluctuation in anti-M{\"u}llerian hormone or antral follicles originated from between-subject fluctuation, or from within subject fluctuation, the intra-class correlation coefficient was calculated. Results Mixed model analysis and Cohen's D (0.12) confirmed the stability of the difference between logantral follicles and loganti-M{\"u}llerian hormone and so confirmed our hypothesis. The good intra-class correlation coefficient (0.73) indicated a small contribution of within-subject variation to anti-M{\"u}llerian hormone fluctuations. Conclusions Fluctuations in anti-M{\"u}llerian hormone levels parallel fluctuations in antral follicles, suggesting that anti-M{\"u}llerian hormone levels are closely linked to variation in the antral follicles. This knowledge adds to the basic understanding of the origin of anti-M{\"u}llerian hormone and could aid in interpretation of individual anti-M{\"u}llerian hormone levels.",

keywords = "Anti-M{\"u}llerian hormone, Antral follicle count, Fluctuation",

author = "Martine Depmann and \{van Disseldorp\}, Jeroen and Broer, \{Simone L.\} and Eijkemans, \{Marinus J C\} and Laven, \{Joop S E\} and Visser, \{Jenny A.\} and \{de Rijke\}, \{Yolanda B.\} and Mol, \{Ben W.\} and Broekmans, \{Frank J M\}",

year = "2016",

month = jul,

doi = "10.1111/aogs.12886",

language = "English",

volume = "95",

pages = "820--828",

journal = "Acta Obstetricia et Gynecologica Scandinavica",

issn = "0001-6349",

publisher = "Wiley-Blackwell",

number = "7",

}

Depmann, M, van Disseldorp, J, Broer, SL, Eijkemans, MJC, Laven, JSE, Visser, JA, de Rijke, YB, Mol, BW & Broekmans, FJM 2016, 'Fluctuations in anti-Müllerian hormone levels throughout the menstrual cycle parallel fluctuations in the antral follicle count: A cohort study', Acta Obstetricia et Gynecologica Scandinavica, vol. 95, no. 7, pp. 820-828. https://doi.org/10.1111/aogs.12886

Fluctuations in anti-Müllerian hormone levels throughout the menstrual cycle parallel fluctuations in the antral follicle count: A cohort study. / Depmann, Martine; van Disseldorp, Jeroen; Broer, Simone L. et al.
In: Acta Obstetricia et Gynecologica Scandinavica, Vol. 95, No. 7, 07.2016, p. 820-828.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Fluctuations in anti-Müllerian hormone levels throughout the menstrual cycle parallel fluctuations in the antral follicle count

T2 - A cohort study

AU - Depmann, Martine

AU - van Disseldorp, Jeroen

AU - Broer, Simone L.

AU - Eijkemans, Marinus J C

AU - Laven, Joop S E

AU - Visser, Jenny A.

AU - de Rijke, Yolanda B.

AU - Mol, Ben W.

AU - Broekmans, Frank J M

PY - 2016/7

Y1 - 2016/7

N2 - Introduction In this prospective cohort study we aimed to investigate the hypothesis that fluctuations in anti-Müllerian hormone levels stem from fluctuations in the number of antral follicles. Material and methods Repeated measurements of anti-Müllerian hormone and antral follicles (follicles 2–8 mm) were performed in 44 women with a regular cycle, during one menstrual cycle. If our hypothesis that anti-Müllerian hormone fluctuations stem from fluctuations in the antral follicles is correct, a fluctuation in the antral follicles would result in an equal and parallel shift in anti-Müllerian hormone. Hence, the difference between antral follicles and anti-Müllerian hormone would remain constant over time. A mixed model analysis, assessing the stability between anti-Müllerian hormone and antral follicles, was performed using the difference between logantral follicles and loganti-Müllerian hormone. Cohen's D was calculated for the largest of fixed effects in order to assess stability in relative distance between antral follicles and anti-Müllerian hormone. To assess if fluctuation in anti-Müllerian hormone or antral follicles originated from between-subject fluctuation, or from within subject fluctuation, the intra-class correlation coefficient was calculated. Results Mixed model analysis and Cohen's D (0.12) confirmed the stability of the difference between logantral follicles and loganti-Müllerian hormone and so confirmed our hypothesis. The good intra-class correlation coefficient (0.73) indicated a small contribution of within-subject variation to anti-Müllerian hormone fluctuations. Conclusions Fluctuations in anti-Müllerian hormone levels parallel fluctuations in antral follicles, suggesting that anti-Müllerian hormone levels are closely linked to variation in the antral follicles. This knowledge adds to the basic understanding of the origin of anti-Müllerian hormone and could aid in interpretation of individual anti-Müllerian hormone levels.

AB - Introduction In this prospective cohort study we aimed to investigate the hypothesis that fluctuations in anti-Müllerian hormone levels stem from fluctuations in the number of antral follicles. Material and methods Repeated measurements of anti-Müllerian hormone and antral follicles (follicles 2–8 mm) were performed in 44 women with a regular cycle, during one menstrual cycle. If our hypothesis that anti-Müllerian hormone fluctuations stem from fluctuations in the antral follicles is correct, a fluctuation in the antral follicles would result in an equal and parallel shift in anti-Müllerian hormone. Hence, the difference between antral follicles and anti-Müllerian hormone would remain constant over time. A mixed model analysis, assessing the stability between anti-Müllerian hormone and antral follicles, was performed using the difference between logantral follicles and loganti-Müllerian hormone. Cohen's D was calculated for the largest of fixed effects in order to assess stability in relative distance between antral follicles and anti-Müllerian hormone. To assess if fluctuation in anti-Müllerian hormone or antral follicles originated from between-subject fluctuation, or from within subject fluctuation, the intra-class correlation coefficient was calculated. Results Mixed model analysis and Cohen's D (0.12) confirmed the stability of the difference between logantral follicles and loganti-Müllerian hormone and so confirmed our hypothesis. The good intra-class correlation coefficient (0.73) indicated a small contribution of within-subject variation to anti-Müllerian hormone fluctuations. Conclusions Fluctuations in anti-Müllerian hormone levels parallel fluctuations in antral follicles, suggesting that anti-Müllerian hormone levels are closely linked to variation in the antral follicles. This knowledge adds to the basic understanding of the origin of anti-Müllerian hormone and could aid in interpretation of individual anti-Müllerian hormone levels.

KW - Anti-Müllerian hormone

KW - Antral follicle count

KW - Fluctuation

UR - https://www.scopus.com/pages/publications/84962765778

U2 - 10.1111/aogs.12886

DO - 10.1111/aogs.12886

M3 - Article

C2 - 26919173

AN - SCOPUS:84962765778

SN - 0001-6349

VL - 95

SP - 820

EP - 828

JO - Acta Obstetricia et Gynecologica Scandinavica

JF - Acta Obstetricia et Gynecologica Scandinavica

IS - 7

ER -

Fluctuations in anti-Müllerian hormone levels throughout the menstrual cycle parallel fluctuations in the antral follicle count: A cohort study

Abstract

Keywords

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