Abstract
The aim of this study was to produce three-dimensional, nanofibrous fish scale/poly(3-hydroxybutyrate-co-3-hydroxyvalerate) composite scaffolds as bone filling materials. This is the first report wherein fish scales were used within a nanofibrous matrix for bone regeneration. Composite scaffolds with a cotton wool-like structure (fiber diameter: 560 ± 64 nm; porosity: 82%) were obtained by incorporating chopped fish scales into wet-electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nanofibers and freeze-drying. The addition of the fish scales improved the mechanical properties, biomineralization tendency, cell viability, alkaline phosphatase activity, and type I collagen production. Consequently, produced composite scaffolds would be regarded to have the therapeutic capacity in bone tissue damages.
| Original language | English |
|---|---|
| Pages (from-to) | 1201-1215 |
| Number of pages | 15 |
| Journal | Journal of biomaterials applications |
| Volume | 34 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Apr 2020 |
| Externally published | Yes |
Keywords
- Alkaline Phosphatase/metabolism
- Animal Scales
- Animals
- Bone Regeneration/drug effects
- Cell Line, Tumor
- Cell Proliferation
- Cell Survival
- Collagen Type I/chemistry
- Fishes
- Freeze Drying
- Humans
- Nanofibers/chemistry
- Osteoblasts/metabolism
- Osteosarcoma/drug therapy
- Phenotype
- Polyesters/chemistry
- Spectroscopy, Fourier Transform Infrared
- Tissue Engineering
- Tissue Scaffolds/chemistry