Fibronectin is a hypoxia-independent target of the tumor suppressor VHL

J.A.R. Bluijssen; M.P.J.K. Lolkema; M. van Beest; M. Boone; C.M.J.T. Snijckers; M. Los; M.F.B.G. Gebbink; B. Braam; F.C.P. Holstege; R.H. Giles; E.E. Voest

Fibronectin is a hypoxia-independent target of the tumor suppressor VHL

J.A.R. Bluijssen, M.P.J.K. Lolkema, M. van Beest, M. Boone, C.M.J.T. Snijckers, M. Los, M.F.B.G. Gebbink, B. Braam, F.C.P. Holstege, R.H. Giles, E.E. Voest

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The von Hippel-Lindau (VHL) tumor suppressor gene regulates the extracellular matrix by controlling fibronectin deposition. To identify novel VHL target genes, we subjected mRNA from VHL-deficient RCC cells (786-0-pRC) and a transfectant re-expressing wildtype VHL (786-0-VHL) to differential expression profiling. Among the differentially expressed genes, we detected that fibronectin is upregulated in the presence of VHL, while it is not affected by hypoxia. Thus regulation of fibronectin deposition by VHL occurs at the transcriptional level, irrespective of oxygen levels.

Original language	English
Pages (from-to)	137-142
Number of pages	6
Journal	FEBS letters
Volume	556
Issue number	1-3
Publication status	Published - 2 Jan 2004

Keywords

Carcinoma, Renal Cell
Cell Hypoxia
Ceruloplasmin
Fibronectins
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Genes, Tumor Suppressor
Humans
Insulin-Like Growth Factor Binding Protein 3
RNA, Messenger
Recombinant Proteins
Transcription, Genetic
Transfection
Tumor Cells, Cultured
Tumor Suppressor Proteins
Ubiquitin-Protein Ligases
Up-Regulation
Vascular Endothelial Growth Factors
Von Hippel-Lindau Tumor Suppressor Protein

Cite this

@article{baecad0ff788432e8de6a7501e3d8534,

title = "Fibronectin is a hypoxia-independent target of the tumor suppressor VHL",

abstract = "The von Hippel-Lindau (VHL) tumor suppressor gene regulates the extracellular matrix by controlling fibronectin deposition. To identify novel VHL target genes, we subjected mRNA from VHL-deficient RCC cells (786-0-pRC) and a transfectant re-expressing wildtype VHL (786-0-VHL) to differential expression profiling. Among the differentially expressed genes, we detected that fibronectin is upregulated in the presence of VHL, while it is not affected by hypoxia. Thus regulation of fibronectin deposition by VHL occurs at the transcriptional level, irrespective of oxygen levels.",

keywords = "Carcinoma, Renal Cell, Cell Hypoxia, Ceruloplasmin, Fibronectins, Gene Expression Profiling, Gene Expression Regulation, Neoplastic, Genes, Tumor Suppressor, Humans, Insulin-Like Growth Factor Binding Protein 3, RNA, Messenger, Recombinant Proteins, Transcription, Genetic, Transfection, Tumor Cells, Cultured, Tumor Suppressor Proteins, Ubiquitin-Protein Ligases, Up-Regulation, Vascular Endothelial Growth Factors, Von Hippel-Lindau Tumor Suppressor Protein",

author = "J.A.R. Bluijssen and M.P.J.K. Lolkema and {van Beest}, M. and M. Boone and C.M.J.T. Snijckers and M. Los and M.F.B.G. Gebbink and B. Braam and F.C.P. Holstege and R.H. Giles and E.E. Voest",

year = "2004",

month = jan,

day = "2",

language = "English",

volume = "556",

pages = "137--142",

journal = "FEBS letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "1-3",

}

TY - JOUR

T1 - Fibronectin is a hypoxia-independent target of the tumor suppressor VHL

AU - Bluijssen, J.A.R.

AU - Lolkema, M.P.J.K.

AU - van Beest, M.

AU - Boone, M.

AU - Snijckers, C.M.J.T.

AU - Los, M.

AU - Gebbink, M.F.B.G.

AU - Braam, B.

AU - Holstege, F.C.P.

AU - Giles, R.H.

AU - Voest, E.E.

PY - 2004/1/2

Y1 - 2004/1/2

N2 - The von Hippel-Lindau (VHL) tumor suppressor gene regulates the extracellular matrix by controlling fibronectin deposition. To identify novel VHL target genes, we subjected mRNA from VHL-deficient RCC cells (786-0-pRC) and a transfectant re-expressing wildtype VHL (786-0-VHL) to differential expression profiling. Among the differentially expressed genes, we detected that fibronectin is upregulated in the presence of VHL, while it is not affected by hypoxia. Thus regulation of fibronectin deposition by VHL occurs at the transcriptional level, irrespective of oxygen levels.

AB - The von Hippel-Lindau (VHL) tumor suppressor gene regulates the extracellular matrix by controlling fibronectin deposition. To identify novel VHL target genes, we subjected mRNA from VHL-deficient RCC cells (786-0-pRC) and a transfectant re-expressing wildtype VHL (786-0-VHL) to differential expression profiling. Among the differentially expressed genes, we detected that fibronectin is upregulated in the presence of VHL, while it is not affected by hypoxia. Thus regulation of fibronectin deposition by VHL occurs at the transcriptional level, irrespective of oxygen levels.

KW - Carcinoma, Renal Cell

KW - Cell Hypoxia

KW - Ceruloplasmin

KW - Fibronectins

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Neoplastic

KW - Genes, Tumor Suppressor

KW - Humans

KW - Insulin-Like Growth Factor Binding Protein 3

KW - RNA, Messenger

KW - Recombinant Proteins

KW - Transcription, Genetic

KW - Transfection

KW - Tumor Cells, Cultured

KW - Tumor Suppressor Proteins

KW - Ubiquitin-Protein Ligases

KW - Up-Regulation

KW - Vascular Endothelial Growth Factors

KW - Von Hippel-Lindau Tumor Suppressor Protein

M3 - Article

C2 - 14706840

SN - 0014-5793

VL - 556

SP - 137

EP - 142

JO - FEBS letters

JF - FEBS letters

IS - 1-3

ER -

Fibronectin is a hypoxia-independent target of the tumor suppressor VHL

Abstract

Keywords

Fingerprint

Cite this