Abstract
Human milk triglycerides (TGs) were separated into 14 fractions by silver ion high-performance liquid chromatography (HPLC) with light-scattering detection (LSD). Subsequent fractionation by reversed-phase HPLC-LSD resulted in 75 subfractions. The major 48 were analysed by gas chromatography for their intact TG and fatty acid (FA) compositions. Using a constrained non-linear optimization computer program, the FA compositions and abundances of 170 different TG combinations were calculated. The major two, 16:0/18:1/18:1 (11.8 mol%) and 16:0/18:1/18:2 (10.0 mol%), were almost twice as abundant as expected from random FA distribution. The apolarities of 16:0 and 18:0 seem to be modulated by unsaturated FAs (18:1, 18:2) or medium-chain FAs (MCFAs; 8:0-12:0). MCFAs were present in 19 mol%. Of these, 7.2 mol% are likely to harbour stereospecific structures with an MCFA at sn-3, 16:0 or 14:0 at sn-2, and 18:1 or 18:2 at sn-1. These structures are excellent substrates for lingual and gastric lipases, producing products that assist in duodenal TG lipolysis.
| Original language | English |
|---|---|
| Pages (from-to) | 9-24 |
| Number of pages | 16 |
| Journal | Journal of chromatography |
| Volume | 616 |
| Issue number | 1 |
| Publication status | Published - 23 Jun 1993 |
Keywords
- Chromatography, Gas
- Chromatography, High Pressure Liquid
- Fatty Acids/analysis
- Female
- Humans
- Infant
- Infant Food/analysis
- Light
- Milk, Human/chemistry
- Scattering, Radiation
- Silver/chemistry
- Triglycerides/analysis