Extraction of Chlamydia pneumoniae DNA from vascular tissue for use in PCR: An evaluation of four procedures

H. F. Berg; B. Maraha; A. M.C. Bergmans; A. van der Zee; J. A.J.W. Kluytmans; M. F. Peeters

doi:10.1046/j.1469-0691.2003.00470.x

Extraction of Chlamydia pneumoniae DNA from vascular tissue for use in PCR: An evaluation of four procedures

H. F. Berg^*
, B. Maraha
, A. M.C. Bergmans
, A. van der Zee
, J. A.J.W. Kluytmans
, M. F. Peeters

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

8 Citations (Scopus)

Abstract

The objective of this study was to compare four procedures for Chlamydia pneumoniae DNA extraction from vascular tissue. The NucliSens Kit, the QIAamp tissue DNA MiniKit, buffer-saturated phenol and the Geneclean II Kit were evaluated, based on the yield of recovered DNA, using PCR to detect C. pneumoniae in vascular tissue. The QIAamp tissue procedure had the highest detection level (0.004 inclusion-forming units/sample). All methods, except NucliSens (70 min), had a short handling time (30-40 min). Costs varied from 0.5 to 3.2 Euro.

Original language	English
Pages (from-to)	135-139
Number of pages	5
Journal	Clinical Microbiology and Infection
Volume	9
Issue number	2
DOIs	https://doi.org/10.1046/j.1469-0691.2003.00470.x
Publication status	Published - 1 Feb 2003
Externally published	Yes

Keywords

Chlamydia pneumoniae
DNA extraction

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10.1046/j.1469-0691.2003.00470.x

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title = "Extraction of Chlamydia pneumoniae DNA from vascular tissue for use in PCR: An evaluation of four procedures",

abstract = "The objective of this study was to compare four procedures for Chlamydia pneumoniae DNA extraction from vascular tissue. The NucliSens Kit, the QIAamp tissue DNA MiniKit, buffer-saturated phenol and the Geneclean II Kit were evaluated, based on the yield of recovered DNA, using PCR to detect C. pneumoniae in vascular tissue. The QIAamp tissue procedure had the highest detection level (0.004 inclusion-forming units/sample). All methods, except NucliSens (70 min), had a short handling time (30-40 min). Costs varied from 0.5 to 3.2 Euro.",

keywords = "Chlamydia pneumoniae, DNA extraction",

author = "Berg, \{H. F.\} and B. Maraha and Bergmans, \{A. M.C.\} and \{van der Zee\}, A. and Kluytmans, \{J. A.J.W.\} and Peeters, \{M. F.\}",

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doi = "10.1046/j.1469-0691.2003.00470.x",

language = "English",

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TY - JOUR

T1 - Extraction of Chlamydia pneumoniae DNA from vascular tissue for use in PCR

T2 - An evaluation of four procedures

AU - Berg, H. F.

AU - Maraha, B.

AU - Bergmans, A. M.C.

AU - van der Zee, A.

AU - Kluytmans, J. A.J.W.

AU - Peeters, M. F.

PY - 2003/2/1

Y1 - 2003/2/1

N2 - The objective of this study was to compare four procedures for Chlamydia pneumoniae DNA extraction from vascular tissue. The NucliSens Kit, the QIAamp tissue DNA MiniKit, buffer-saturated phenol and the Geneclean II Kit were evaluated, based on the yield of recovered DNA, using PCR to detect C. pneumoniae in vascular tissue. The QIAamp tissue procedure had the highest detection level (0.004 inclusion-forming units/sample). All methods, except NucliSens (70 min), had a short handling time (30-40 min). Costs varied from 0.5 to 3.2 Euro.

AB - The objective of this study was to compare four procedures for Chlamydia pneumoniae DNA extraction from vascular tissue. The NucliSens Kit, the QIAamp tissue DNA MiniKit, buffer-saturated phenol and the Geneclean II Kit were evaluated, based on the yield of recovered DNA, using PCR to detect C. pneumoniae in vascular tissue. The QIAamp tissue procedure had the highest detection level (0.004 inclusion-forming units/sample). All methods, except NucliSens (70 min), had a short handling time (30-40 min). Costs varied from 0.5 to 3.2 Euro.

KW - Chlamydia pneumoniae

KW - DNA extraction

UR - https://www.scopus.com/pages/publications/0038414797

U2 - 10.1046/j.1469-0691.2003.00470.x

DO - 10.1046/j.1469-0691.2003.00470.x

M3 - Article

C2 - 12588334

AN - SCOPUS:0038414797

SN - 1198-743X

VL - 9

SP - 135

EP - 139

JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

IS - 2

ER -

Extraction of Chlamydia pneumoniae DNA from vascular tissue for use in PCR: An evaluation of four procedures

Abstract

Keywords

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