TY - JOUR
T1 - Extraction of Chlamydia pneumoniae DNA from vascular tissue for use in PCR
T2 - An evaluation of four procedures
AU - Berg, H. F.
AU - Maraha, B.
AU - Bergmans, A. M.C.
AU - van der Zee, A.
AU - Kluytmans, J. A.J.W.
AU - Peeters, M. F.
PY - 2003/2/1
Y1 - 2003/2/1
N2 - The objective of this study was to compare four procedures for Chlamydia pneumoniae DNA extraction from vascular tissue. The NucliSens Kit, the QIAamp tissue DNA MiniKit, buffer-saturated phenol and the Geneclean II Kit were evaluated, based on the yield of recovered DNA, using PCR to detect C. pneumoniae in vascular tissue. The QIAamp tissue procedure had the highest detection level (0.004 inclusion-forming units/sample). All methods, except NucliSens (70 min), had a short handling time (30-40 min). Costs varied from 0.5 to 3.2 Euro.
AB - The objective of this study was to compare four procedures for Chlamydia pneumoniae DNA extraction from vascular tissue. The NucliSens Kit, the QIAamp tissue DNA MiniKit, buffer-saturated phenol and the Geneclean II Kit were evaluated, based on the yield of recovered DNA, using PCR to detect C. pneumoniae in vascular tissue. The QIAamp tissue procedure had the highest detection level (0.004 inclusion-forming units/sample). All methods, except NucliSens (70 min), had a short handling time (30-40 min). Costs varied from 0.5 to 3.2 Euro.
KW - Chlamydia pneumoniae
KW - DNA extraction
UR - http://www.scopus.com/inward/record.url?scp=0038414797&partnerID=8YFLogxK
U2 - 10.1046/j.1469-0691.2003.00470.x
DO - 10.1046/j.1469-0691.2003.00470.x
M3 - Article
C2 - 12588334
AN - SCOPUS:0038414797
SN - 1198-743X
VL - 9
SP - 135
EP - 139
JO - Clinical Microbiology and Infection
JF - Clinical Microbiology and Infection
IS - 2
ER -