TY - JOUR
T1 - Expression of the glucocorticoid receptor gene is regulated during early embryogenesis of Xenopus laevis
AU - Gao, Xiangming
AU - Kalkhoven, Eric
AU - Peterson-Maduro, Joselin
AU - van der Burg, Bart
AU - Destrée, Olivier H.J.
PY - 1994/6/21
Y1 - 1994/6/21
N2 - To study the possible role of the glucocorticoid receptor (GR) in early embryogenesis, we isolated a Xenopus glucocorticoid receptor cDNA from an embryonic stage 17 cDNA library. Overexpression of this Xenopus GR in COS cells confers the ability to transactivate a GRE-tk CAT promoter construct in a ligand dependent manner. Expression of the Xenopus GR gene at the RNA level was analyzed by Northern blot hybridization. Transcripts of 4 and 6 kb are present in oocytes. The 4 kb mRNA is abundant and is degraded together with the 6 kb mRNA during cleavage stages of early development. Between stages 17 and 24, GR messengers are extremely rare. From stage 32 onwards, both GR transcripts start to be expressed again at intermediate levels. These results provide the first evidence that expression of the GR gene is regulated during early embryonic development.
AB - To study the possible role of the glucocorticoid receptor (GR) in early embryogenesis, we isolated a Xenopus glucocorticoid receptor cDNA from an embryonic stage 17 cDNA library. Overexpression of this Xenopus GR in COS cells confers the ability to transactivate a GRE-tk CAT promoter construct in a ligand dependent manner. Expression of the Xenopus GR gene at the RNA level was analyzed by Northern blot hybridization. Transcripts of 4 and 6 kb are present in oocytes. The 4 kb mRNA is abundant and is degraded together with the 6 kb mRNA during cleavage stages of early development. Between stages 17 and 24, GR messengers are extremely rare. From stage 32 onwards, both GR transcripts start to be expressed again at intermediate levels. These results provide the first evidence that expression of the GR gene is regulated during early embryonic development.
KW - (Xenopus embryo)
KW - Expression
KW - Functional assay
KW - Gene cloning
KW - Glucocorticoid receptor
KW - Sequence
UR - http://www.scopus.com/inward/record.url?scp=0028291822&partnerID=8YFLogxK
U2 - 10.1016/0167-4781(94)90010-8
DO - 10.1016/0167-4781(94)90010-8
M3 - Article
C2 - 8018720
AN - SCOPUS:0028291822
SN - 0167-4781
VL - 1218
SP - 194
EP - 198
JO - BBA - Gene Structure and Expression
JF - BBA - Gene Structure and Expression
IS - 2
ER -