Expression of CD64 (Fc gamma RI) in skin of patients with acute GVHD

A. van Royen-Kerkhof; V. Walraven; E.A.M. Sanders; R.A. de Weger; D.F. van Wichen; E. de Koning; T. Thepen; J.G.J. van de Winkel; J.H.W. Leusen

doi:10.1038/bmt.2010.337

Expression of CD64 (Fc gamma RI) in skin of patients with acute GVHD

A. van Royen-Kerkhof, V. Walraven, E.A.M. Sanders, R.A. de Weger, D.F. van Wichen, E. de Koning, T. Thepen, J.G.J. van de Winkel, J.H.W. Leusen

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

GVHD remains a major problem in allo-SCT. We explored the presence of APC in skin biopsies of GVHD patients, using the IgG receptor CD64 expression as a hallmark for activated APC. By immunohistochemistry we demonstrated CD64 to be upregulated on host APC in skin biopsies of patients with acute GVHD and, less prominently, in chronic GVHD. Double staining for CD32 polymorphism revealed CD64-positive cells to be mainly of host origin. The majority of CD64-positive cells coexpressed CD68, indicating a macrophage phenotype. Given its very restricted cellular distribution, CD64 may represent an excellent target for APC-directed therapies in GVHD. Bone Marrow Transplantation (2011) 46, 1566-1569; doi:10.1038/bmt.2010.337; published online 17 January 2011

Original language	English
Pages (from-to)	1566-1569
Number of pages	4
Journal	Bone Marrow Transplantation
Volume	46
Issue number	12
DOIs	https://doi.org/10.1038/bmt.2010.337
Publication status	Published - Dec 2011

Keywords

graft-versus-host disease
IgG receptor
CD64
immunotherapy
macrophage
VERSUS-HOST-DISEASE
DENDRITIC CELLS
MACROPHAGES
RECEPTOR
TRANSPLANTATION
POLYMORPHISM

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10.1038/bmt.2010.337

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@article{14fded477ef84fa38eb3a77e3ddb43af,

title = "Expression of CD64 (Fc gamma RI) in skin of patients with acute GVHD",

abstract = "GVHD remains a major problem in allo-SCT. We explored the presence of APC in skin biopsies of GVHD patients, using the IgG receptor CD64 expression as a hallmark for activated APC. By immunohistochemistry we demonstrated CD64 to be upregulated on host APC in skin biopsies of patients with acute GVHD and, less prominently, in chronic GVHD. Double staining for CD32 polymorphism revealed CD64-positive cells to be mainly of host origin. The majority of CD64-positive cells coexpressed CD68, indicating a macrophage phenotype. Given its very restricted cellular distribution, CD64 may represent an excellent target for APC-directed therapies in GVHD. Bone Marrow Transplantation (2011) 46, 1566-1569; doi:10.1038/bmt.2010.337; published online 17 January 2011",

keywords = "graft-versus-host disease, IgG receptor, CD64, immunotherapy, macrophage, VERSUS-HOST-DISEASE, DENDRITIC CELLS, MACROPHAGES, RECEPTOR, TRANSPLANTATION, POLYMORPHISM",

author = "{van Royen-Kerkhof}, A. and V. Walraven and E.A.M. Sanders and {de Weger}, R.A. and {van Wichen}, D.F. and {de Koning}, E. and T. Thepen and {van de Winkel}, J.G.J. and J.H.W. Leusen",

year = "2011",

month = dec,

doi = "10.1038/bmt.2010.337",

language = "English",

volume = "46",

pages = "1566--1569",

journal = "Bone Marrow Transplantation",

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publisher = "Nature Publishing Group",

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TY - JOUR

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AU - van Royen-Kerkhof, A.

AU - Walraven, V.

AU - Sanders, E.A.M.

AU - de Weger, R.A.

AU - van Wichen, D.F.

AU - de Koning, E.

AU - Thepen, T.

AU - van de Winkel, J.G.J.

AU - Leusen, J.H.W.

PY - 2011/12

Y1 - 2011/12

N2 - GVHD remains a major problem in allo-SCT. We explored the presence of APC in skin biopsies of GVHD patients, using the IgG receptor CD64 expression as a hallmark for activated APC. By immunohistochemistry we demonstrated CD64 to be upregulated on host APC in skin biopsies of patients with acute GVHD and, less prominently, in chronic GVHD. Double staining for CD32 polymorphism revealed CD64-positive cells to be mainly of host origin. The majority of CD64-positive cells coexpressed CD68, indicating a macrophage phenotype. Given its very restricted cellular distribution, CD64 may represent an excellent target for APC-directed therapies in GVHD. Bone Marrow Transplantation (2011) 46, 1566-1569; doi:10.1038/bmt.2010.337; published online 17 January 2011

AB - GVHD remains a major problem in allo-SCT. We explored the presence of APC in skin biopsies of GVHD patients, using the IgG receptor CD64 expression as a hallmark for activated APC. By immunohistochemistry we demonstrated CD64 to be upregulated on host APC in skin biopsies of patients with acute GVHD and, less prominently, in chronic GVHD. Double staining for CD32 polymorphism revealed CD64-positive cells to be mainly of host origin. The majority of CD64-positive cells coexpressed CD68, indicating a macrophage phenotype. Given its very restricted cellular distribution, CD64 may represent an excellent target for APC-directed therapies in GVHD. Bone Marrow Transplantation (2011) 46, 1566-1569; doi:10.1038/bmt.2010.337; published online 17 January 2011

KW - graft-versus-host disease

KW - IgG receptor

KW - CD64

KW - immunotherapy

KW - macrophage

KW - VERSUS-HOST-DISEASE

KW - DENDRITIC CELLS

KW - MACROPHAGES

KW - RECEPTOR

KW - TRANSPLANTATION

KW - POLYMORPHISM

U2 - 10.1038/bmt.2010.337

DO - 10.1038/bmt.2010.337

M3 - Article

C2 - 21243031

SN - 0268-3369

VL - 46

SP - 1566

EP - 1569

JO - Bone Marrow Transplantation

JF - Bone Marrow Transplantation

IS - 12

ER -

Expression of CD64 (Fc gamma RI) in skin of patients with acute GVHD

Abstract

Keywords

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