Experimental hypercalcemia induces hypocalcin release and inhibits branchial Ca+ influx in freshwater trout

F. P.J.G. Lafeber; S. F. Perry

doi:10.1016/0016-6480(88)90189-X

Experimental hypercalcemia induces hypocalcin release and inhibits branchial Ca⁺ influx in freshwater trout

F. P.J.G. Lafeber^*, S. F. Perry

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

39 Citations (Scopus)

Abstract

Intravascular CaCl₂ infusion in freshwater rainbow trout (Salmo gairdneri) causes a significant degranulation of the corpuscles of Stannius (CS). Concurrently, there is a specific and acute inhibition of whole body Ca²⁺ influx; Ca²⁺ efflux is not effected. The material released from the CS after CaCl₂ injection consists primarily of a 28-kDa product which we identified as hypocalcin. Electron microscope observations of the CS reveal that type 1 and type 2 cells are degranulated to a similar extent. We conclude that hypocalcin is directly involved in hypocalcemic control in freshwater fish via inhibition of branchial Ca²⁺ influx, thereby promoting a net loss of Ca²⁺ across the gill.

Original language	English
Pages (from-to)	136-143
Number of pages	8
Journal	General and comparative endocrinology
Volume	72
Issue number	1
DOIs	https://doi.org/10.1016/0016-6480(88)90189-X
Publication status	Published - Oct 1988
Externally published	Yes

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@article{c057c8c9419d42f3a289228b0994851e,

title = "Experimental hypercalcemia induces hypocalcin release and inhibits branchial Ca+ influx in freshwater trout",

abstract = "Intravascular CaCl2 infusion in freshwater rainbow trout (Salmo gairdneri) causes a significant degranulation of the corpuscles of Stannius (CS). Concurrently, there is a specific and acute inhibition of whole body Ca2+ influx; Ca2+ efflux is not effected. The material released from the CS after CaCl2 injection consists primarily of a 28-kDa product which we identified as hypocalcin. Electron microscope observations of the CS reveal that type 1 and type 2 cells are degranulated to a similar extent. We conclude that hypocalcin is directly involved in hypocalcemic control in freshwater fish via inhibition of branchial Ca2+ influx, thereby promoting a net loss of Ca2+ across the gill.",

author = "Lafeber, {F. P.J.G.} and Perry, {S. F.}",

note = "Funding Information: We thank Tamara Grossmann (Canada) and Coen Van der Meij (The Netherlands) for skillful assistance during the experiments. We thank Professor Dr. S . E. Wendelaar Bonga and Dr. G. Flik for critical comments during preparation of the manuscript. This study was supported by an NSERC of Canada operating grant to S. F. Perry and by the Foundation for Fundamental Biological Research (BION), which is subsidized by the Dutch Organization for the Advancement of Pure Research (ZWO).",

year = "1988",

month = oct,

doi = "10.1016/0016-6480(88)90189-X",

language = "English",

volume = "72",

pages = "136--143",

number = "1",

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TY - JOUR

T1 - Experimental hypercalcemia induces hypocalcin release and inhibits branchial Ca+ influx in freshwater trout

AU - Lafeber, F. P.J.G.

AU - Perry, S. F.

N1 - Funding Information: We thank Tamara Grossmann (Canada) and Coen Van der Meij (The Netherlands) for skillful assistance during the experiments. We thank Professor Dr. S . E. Wendelaar Bonga and Dr. G. Flik for critical comments during preparation of the manuscript. This study was supported by an NSERC of Canada operating grant to S. F. Perry and by the Foundation for Fundamental Biological Research (BION), which is subsidized by the Dutch Organization for the Advancement of Pure Research (ZWO).

PY - 1988/10

Y1 - 1988/10

N2 - Intravascular CaCl2 infusion in freshwater rainbow trout (Salmo gairdneri) causes a significant degranulation of the corpuscles of Stannius (CS). Concurrently, there is a specific and acute inhibition of whole body Ca2+ influx; Ca2+ efflux is not effected. The material released from the CS after CaCl2 injection consists primarily of a 28-kDa product which we identified as hypocalcin. Electron microscope observations of the CS reveal that type 1 and type 2 cells are degranulated to a similar extent. We conclude that hypocalcin is directly involved in hypocalcemic control in freshwater fish via inhibition of branchial Ca2+ influx, thereby promoting a net loss of Ca2+ across the gill.

AB - Intravascular CaCl2 infusion in freshwater rainbow trout (Salmo gairdneri) causes a significant degranulation of the corpuscles of Stannius (CS). Concurrently, there is a specific and acute inhibition of whole body Ca2+ influx; Ca2+ efflux is not effected. The material released from the CS after CaCl2 injection consists primarily of a 28-kDa product which we identified as hypocalcin. Electron microscope observations of the CS reveal that type 1 and type 2 cells are degranulated to a similar extent. We conclude that hypocalcin is directly involved in hypocalcemic control in freshwater fish via inhibition of branchial Ca2+ influx, thereby promoting a net loss of Ca2+ across the gill.

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U2 - 10.1016/0016-6480(88)90189-X

DO - 10.1016/0016-6480(88)90189-X

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AN - SCOPUS:0023694901

SN - 0016-6480

VL - 72

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EP - 143

JO - General and comparative endocrinology

JF - General and comparative endocrinology

IS - 1

ER -

Experimental hypercalcemia induces hypocalcin release and inhibits branchial Ca⁺ influx in freshwater trout

Abstract

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